Evaluation of an IgG-ELISA strategy using Taenia solium metacestode somatic and excretory–secretory antigens for diagnosis of neurocysticercosis revealing biological stage of the larvae

Sahu, Priyadarshi Soumyaranjan; Parija, Subhash Chandra; Narayan, Shyam; Kumar, D.

doi:10.1016/j.actatropica.2009.01.002

Cited by 34 publications

(36 citation statements)

References 39 publications

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“…In a study of Sahu et al, serum performed better than CSF when using E/S antigens but there were no differences when using somatic antigens. 108 In general, the evidence suggests that with properly specific assays, serum performs better than CSF for antibody detection. 10,46,48,50,109,110 Some authors note that the simultaneous use of both samples can provide important information on the infection status of the patient.…”

Section: Appropriate Samples For Antibody Detectionmentioning

confidence: 99%

Immunological and molecular diagnosis of cysticercosis

Rodríguez¹,

Wilkins

Dorny

2012

Pathogens and Global Health

137

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Cysticercosis, the infection with the larval stage of Taenia solium, is a cause of neurological symptoms including seizures, affecting the quality of life of patients and their families. Diagnosis focuses on brain imaging and serological tests are mostly used as confirmatory tools. Most cases, however, occur in poor endemic areas, where both kinds of diagnostic tools are poorly available. Development of point of care diagnostic tests is one of the most important priorities for cysticercosis researches today. The ideal point of care test would require detection of viable cysticercosis and hopefully identify cases with severe or progressive forms of neurocysticercosis, leading to referral of the patient for specialized medical attention. This manuscript describes the evolution of the serological diagnosis of cysticercosis over time, and the characteristics of the most common currently available tools, their advantages and disadvantages, and their potential use in future diagnostic tests.

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Section: Appropriate Samples For Antibody Detectionmentioning

confidence: 99%

Immunological and molecular diagnosis of cysticercosis

Rodríguez¹,

Wilkins

Dorny

2012

Pathogens and Global Health

137

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“…The diagnosis of human NC is determined after the analysis of clinical, epidemiological, neuroimaging and immunological data (Del Brutto et al 2001). The combination of enzyme linked immunosorbent assay (ELISA) and western blotting (WB) to detect specific antibodies against metacestodes extracts of T. solium in the serum samples or in the cerebrospinal fluid (CSF) of patients with NC represents a viable alternative for the diagnosis of the disease in developing countries (Shiguekawa et al 2000, Barcelos et al 2001, Sahu et al 2009, Nunes et al 2010.…”

mentioning

confidence: 99%

Genetic polymorphism in Taenia solium metacestodes from different Brazilian geographic areas

Barcelos

Souza

Pena

et al. 2012

Mem. Inst. Oswaldo Cruz

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“…Also, it is important to note the technique is very expensive, hampering its use in endemic areas (Villota et al 2003;Suzuki and Rossi 2011).The EITB was recognized as the gold standard for NCC immunodiagnosis by Pan American Health Organization (PAHO) (Greene et al 2000). Also, purified T. solium E/S antigens have been employed in ELISA and FAST-ELISA with very promising results in antibody detection of NCC (Ng and Ko 1994;Sahu et al 2009;Atluri et al 2009). From the metacestode secretion proteins, those that have low molecular weight, and especially glycoproteins, have showed the best performances in NCC diagnosis assays.…”

Section: Immunodiagnosismentioning

confidence: 98%

Taeniosis and Cysticercosis

Ferrer

Gárate

2014

Helminth Infections and Their Impact on Global Public Health

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Taeniosis and cysticercosis are zoonotic diseases produced by Taenia saginata and Taenia solium. The adult tapeworms are parasites of human intestine and show a wide geographical distribution. Taenia asiatica, another tapeworm species, was described in Southeast Asia. The larval stages of these cestodes (metacestodes or cysticerci) cause cysticercosis; T. saginata causes bovine cysticercosis, T. asiatica larvae develop in the pig viscera, and T. solium is able to produce cysticercosis in both pig and man. When the parasite larva invades, the central nervous system (CNS) can provoke neurocysticercosis (NCC), one of the most frequent parasitic infections of human CNS. These diseases continue to cause health problems and livestock industry losses in areas where the parasites are endemic and also in non-endemic regions as a consequence of travel and migrations. There are few symptoms associated with taeniosis; in contrast, NCC (pleomorphic pathology) could be a life-threatening disease, depending on the location, number, stage of cysticerci, and the host immune response. Diagnosis of taeniosis is generally achieved by stool microscopic examinations, and the detection of cysticercosis is generally performed by neuroimaging and immunoassays. Both conventional coprological techniques and immunological assays show limitations, and new diagnostic tools have been developed, more specific and sensitive, such as specific monoclonal antibodies, recombinant antigens, synthetic peptides, and PCR. Considering the clinical impact, veterinary problems, and economic losses derived from taeniosis/cysticercosis, control programs have been implemented. In addition, several vaccine candidates have been characterized that could complement the control measures.

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Evaluation of an IgG-ELISA strategy using Taenia solium metacestode somatic and excretory–secretory antigens for diagnosis of neurocysticercosis revealing biological stage of the larvae

Cited by 34 publications

References 39 publications

Immunological and molecular diagnosis of cysticercosis

Immunological and molecular diagnosis of cysticercosis

Genetic polymorphism in Taenia solium metacestodes from different Brazilian geographic areas

Taeniosis and Cysticercosis

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