Evaluation of an immunochromatographic assay for the detection of anti-hepatitis A virus IgM

Abstract

BackgroundHepatitis A virus (HAV) is a causative agent of acute hepatitis, which is transmitted by person-to-person contact and via the faecal-oral route. Acute HAV infection is usually confirmed by anti-HAV IgM detection. In order to detect anti-HAV IgM in the serum of patients infected with HAV, we developed a rapid assay based on immunochromatography (ICA) and evaluated the sensitivity of this assay by comparing it with a commercial microparticle enzyme immunoassay (MEIA) that is widely used for serological diagnosis.ResultsThe newly developed ICA showed 100% sensitivity and specificity when used to test 150 anti-HAV IgM-positive sera collected from infected patients and 75 negative sera from healthy subjects. Also, the sensitivity of ICA is about 10 times higher than MEIA used in this study by determining end point to detect independent on infected genotype of HAV. In addition, the ICA was able to detect 1 positive sample from among 50 sera from acute hepatitis patients that had tested negative for anti-HAV IgM using the MEIA.ConclusionConclusively, ICA for the detection of anti-HAV IgM will be very effective for rapid assay to apply clinical diagnosis and epidemiological investigation on epidemics due to the simplicity, rapidity and specificity.

Detection of Antibodies against Hepatitis A Virus (HAV) by a Surface Plasmon Resonance (SPR) Biosensor: A New Diagnosis Tool Based on the Major HAV Capsid Protein VP1 (SPR-HAVP1)

“…Currently, this diagnosis is mainly based on ELISA and chemiluminescence immunoassays. Although these assays show good sensitivities and can be automated, they are not high-throughput assays and do not allow large-scale testing [ 8 ]. Most of these immunodiagnostic tests for anti-HAV detection rely on the use of inactivated HAV particles as a tool for antibody detection [ 21 ].…”

“…The current epidemiological scenario of hepatitis A in many countries calls for introduction of new rapid and large-scale methodologies aiming to improve vaccination coverage, specifically within this risk group [ 7 ]. The standard diagnosis of acute hepatitis A is based on serum anti-HAV IgM detection [ 8 ], which is typically detectable at the onset of symptoms and can persist for up to 2–9 months post-infection. IgG antibody follows IgM response after 1 week and provides life-long protection against [ 9 ].…”

Batman Devlet Hastanesine Başvuran Kişilerde Hepatit ve HIV Serolojisinin Sonuçları

“…Çocuklardaki HAV enfeksiyonu genelde asemptomatik iken, erişkinlerdeki enfeksiyon sıklıkla ciddi seyreder (28)(29)(30).…”

Comparison of PCR with Serology for Detecting Acute Hepatitis A Virus Infection

“…Detection of elevated anti-HAV IgM antibodies is the gold standard for diagnosing acute HAV infection. About two weeks following the disease onset, anti-HAV IgG antibodies become detectable in the patient's blood [21]. These antibodies remain for the rest of the life and protect the recovered person against reinfections [21].…”

“…About two weeks following the disease onset, anti-HAV IgG antibodies become detectable in the patient's blood [21]. These antibodies remain for the rest of the life and protect the recovered person against reinfections [21]. In seroepidemiological studies, anti-HAV IgG antibodies indicate previous and inactive infections [21].…”