Evaluation of an inactivated infectious bursal disease virus vaccine prepared using a local isolate from Egypt

The currentInfectious bursal disease virus(IBDV) vaccine strain of LC-75 in Ethiopia is being produced through chicken embryo fibro blast cell which has short lifespan and limited need of specific pathogen free eggs. Ideally, vaccine should have better longevity and being effective in cost and time. Experimental research was conducted to adopt DF-1 cell and to validate the immune responses, immunosuppressive effect and immunogenicity tests, of LC-75 vaccinal strain ofInfections bursal diseases virusA total of 76 chickens were used for these experiments. The seroconversion rate of the vaccine were measured using haemaglutination inhibition (HI) test in the vaccinated two experiment groups, adapted IBDV and Newcastle vaccines at two weeks interval for the first group and only Newcastle vaccine for the second experiment groups. To perform the immunogenicity test, two groups having 20 chickens per group were used and samples of serum were taken on 0-, 7-, 14- and 28-days post vaccinations and subjected to indirect ELISA test. The group that received both vaccine types produced haemagglutination inhibition titer (HIT) of 97.33±22.49 whereas the other group that received only Newcastle vaccine produced 124±24.92. The control group always showed no detectable antibody while the vaccinated group was able to produce average antibody S/P values of 0.00 ± 0.01 at day 0, 0.02± 0.01 at day 7, 1.05±0.10 at day 14 and 0.83±0.05 at day 28. RT-PCR using a 400 bp IBDV viral protein 2(VP2) specific primer resulted from positive bands in all samples. In conclusion, the vaccinal strain was able to replicate and adapt on the DF-1 cells and it was found to be immunogenic as well as less immunosuppressive.

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Stinging nettle and neem enhance antibody response to local killed and imported live infectious bursal disease vaccines in indigenous chicken in Kenya

Bwana

Njagi

Nyaga

et al. 2018

Poultry Science

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Immune responses are critical for protection of chickens from infectious bursal disease (IBD). In this study, the antibody response-enhancing effect of drinking water supplementation of 1% stinging nettle and neem on different IBD vaccines and vaccination regimes was evaluated, using 36 (n = 36) specific antibody negative indigenous chicks. The birds were allocated into 3 groups as follows: 1A-C, 2A-C, and 3A-B, while group 3C acted as the unvaccinated non-supplemented control. A local inactivated K1 and imported live attenuated D78 IBD vaccines were given to groups 1A-C and 3A-B at 14 and 28 d of age, respectively. A combination of K1 and D78 vaccines was given 30 d apart to groups 2A and 2B (D78 at 14 and 21 d and K1 at 44 d of age) and on the same d to group 2C at 14 and 28 d of age. Stinging nettle was given in water to groups 1B, 2B, and 2C, and neem to groups 1C, 2A, and 3B. Birds were bled weekly and immune responses monitored using indirect ELISA. Both neem and stinging nettle had antibody response-enhancing effects in groups 1B and 1C, receiving the local inactivated K1 vaccine. There were significant differences (P < 0.05) in antibody titers between groups 1A and 2C. Stinging nettle induced earlier onset of high antibody responses in group 2C and persistent titers (>3.8 log10) from the third week in group 2B. Imported live D78 vaccine induced higher antibody titers compared to the local inactivated K1 vaccine. Groups 2B and 2C receiving a combination of the local K1 and imported live attenuated D78 vaccines had the highest antibody titers. Adoption of stinging nettle supplementation and a prime-boost program involving use of a local virus isolates-derived vaccine is recommended.

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Evaluation of an inactivated infectious bursal disease virus vaccine prepared using a local isolate from Egypt

Cited by 3 publications

References 14 publications

Evaluation of DF-1 cell culture based vaccine development for infectious bursal disease virus in Ethiopia

Evaluation of DF-1 cell culture based vaccine development for infectious bursal disease virus in Ethiopia

Df-1 Cell Adaptation and Immune Responses of Lc-75 Vaccinal Strain ofInfectious Bursal Disease Virusin Chicken

Stinging nettle and neem enhance antibody response to local killed and imported live infectious bursal disease vaccines in indigenous chicken in Kenya

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