Evaluation of an Isolate ofMyrothecium verrucariafrom Sicklepod (Senna obtusifolia) as a Potential Mycoherbicide Agent

Walker, H. L.; Tilley, Anthony M.

doi:10.1006/bcon.1997.0559

Cited by 63 publications

(64 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus far, the species M. roridum Tode ex Fries, a common soil fungus, has been reported to be pathogenic to a number of plant species. Walker and Tilley (1997) suggested that some Myrothecium species might be used as broad-spectrum bioherbicides against several weeds. However, there is little information about bioherbicidal activity against various weeds in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…Of these Myrothecium species, mainly M. verrucaria has been studied as a potential biocontrol agent for control of, for example, invasive climbing fern (Lygodium microphyllum), dandelion (Taraxacum officinale) (Clarke et al, 2007), kudzu (Boyette et al, 2002), and amaranth weeds including A. hybridus, A. spinosus, and A. lividus (Yang and Brenner, 1997) on the basis of their pathogenicity to host. Recent studies suggest that some Myrothecium species may serve as broad-spectrum bioherbicides (Walker and Tilley, 1997). It has been known that M. roridum may be very opportunistic and may cause severe damage to leaves and stems.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of a Fungal Strain, Myrothecium roridum F0252, as a Bioherbicide Agent

Lee¹,

Kim²,

Hong³

et al. 2008

The Plant Pathology Journal

View full text Add to dashboard Cite

In the course of in vitro and in vivo screening for bioherbicidal agents, a hyphomycete fungus, Myrothecium sp. F0252 was selected as a candidate for the biocontrol of weeds. The isolate was identified as Myrothecium roridum Tode ex. Fries based on the morphological characteristics and 18S ribosomal DNA sequence analysis and registered as Myrothecium roridum F0252. In order to evaluate the in vitro effect of M. roridum F0252 on germination of ladino clover and white clover (Trifolium repens L.) seeds, spore solution of the fungus was employed in two concentrations, 6.5×10 6 and 2.5× 10 7 spores per mL and then inoculated to the seeds. The fungal spores inhibited the seed germination, infected the seedlings, and caused an abnormal withering and inhibition of seedling growth. In addition, when the herbicidal activity of crude ethyl acetate extract from the liquid culture was assessed on a mini-plant, duckweed (Lemna paucicostata (L.) Hegelm.), the extract showed high inhibitory effect at the level of 12.5 µg per mL. On the other hand, in vivo herbicidal activity of M. roridum F0252 was evaluated by a whole plant spray method. M. roridum F0252 exhibited strong and broadspectrum herbicidal activity. The herbicidal values ranged from 95-100% against 7 weeds, including Abutilon avicennae and Xanthium strumarium, and 70-80% against Digitaria sanguinalis and Sagittaria pygmaea. When the nutritional utilization (95 carbon sources) pattern of M. roridum F0252 was investigated, it varied with water activity (a w ) and temperature conditions, supplying good, basic information in regard to nutritional utilization for proper cultivation and formulation. Our results showed that M. roridum F0252 might be used as a potential biocontrol agent against weedy plants.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Evaluation of a Fungal Strain, Myrothecium roridum F0252, as a Bioherbicide Agent

Lee¹,

Kim²,

Hong³

et al. 2008

The Plant Pathology Journal

View full text Add to dashboard Cite

show abstract

“…This strain was originally isolated from diseased sicklepod (Senna obtusifolia) [40]. The fungus was sub-cultured in Petri dishes containing potato dextrose agar (PDA, Difco Laboratories, Detroit, MI, USA).…”

Section: Isolation and Culture Of Myrothecium Verrucariamentioning

confidence: 99%

“…Ditmar:Fr. (strain IMI368023) (MV) has been shown to have bioherbicidal activity on several weeds [40] [41] [42]. Other studies in our laboratory showed MV had bioherbicidal activity against several weeds including: Kudzu (Pueraria lobata var.…”

Section: Introductionmentioning

confidence: 99%

Extending the Shelf-Life of <i>Myrothecium verrucaria</i>, a Bioherbicide

Hoagland¹,

Boyette²,

Stetina³

2017

AJPS

View full text Add to dashboard Cite

The shelf-life of a bioherbicide product is an important factor with regard to its commercial potential. The bioherbicidal efficacy of freshly fermented Myrothecium verrucaria (strain IMI 368023) (MV) mycelia formulations and MV mycelia preparations that had been freeze-dried and then stored at −20˚C for 8 years was compared. Two concentrations of each formulation (1.0x and 0.5x) were tested, utilizing bioassays on seedlings of the weed, hemp sesbania (Sesbania exaltata) under greenhouse conditions or in darkness utilizing hydroponically grown seedlings. Freeze drying of freshly prepared MV mycelium produced a light, brownish-colored powder. Efficacy tests of this reconstituted 8-year-old dried material showed that some bioherbicidal activity was lost during long-term storage, i.e., ~20% and ~60% seedling dry weight reduction at the 1.0x and 0.5x rate, respectively. Although plant mortality was greater in the fresh mycelial preparations treatments versus the freeze-dried and stored samples at all time points in the time-course, the stored material still caused >80% mortality, 15 days after treatment. Comparative disease progression ratings also showed a similar trend. Overall results show that freeze-drying MV is a useful method to reduce the bulk and cumbersomeness of storing heavy liquid fermentation product, while retaining bioherbicidal activity. These findings increase the utility of this bioherbicide and offer the potential to use the dried material in soil treatments or in a more concentrated form than attainable via the fermented product.

show abstract

“…Simple trichothecenes, such as neosoliniol monoacetate and diacetoxy-scripenol, were phytotoxic to pea seedlings, but wheat was tolerant (Brain et al, 1961). A Myrothecium verrucaria isolate that produces verrucarins also has efficacious bioherbicidal activity (Anderson and Hallett, 2004;Boyette et al, 2002;Hoagland et al, 2004;Walker and Tilley, 1997). More data and future research protocols on the development of this fungus as a bioherbicide have recently been published (Hoagland et al, 2007).…”

Section: Moniliformin Moniliformin Occurs In Isolates Of Saprophyticmentioning

confidence: 99%

Bioherbicides: Research and Risks

Hoagland¹,

Boyette²,

Weaver³

et al. 2007

Toxin Reviews

View full text Add to dashboard Cite

Evaluation of an Isolate ofMyrothecium verrucariafrom Sicklepod (Senna obtusifolia) as a Potential Mycoherbicide Agent

Cited by 63 publications

References 19 publications

Evaluation of a Fungal Strain, Myrothecium roridum F0252, as a Bioherbicide Agent

Evaluation of a Fungal Strain, Myrothecium roridum F0252, as a Bioherbicide Agent

Extending the Shelf-Life of <i>Myrothecium verrucaria</i>, a Bioherbicide

Bioherbicides: Research and Risks

Contact Info

Product

Resources

About

Evaluation of an Isolate ofMyrothecium verrucariafrom Sicklepod (Senna obtusifolia) as a Potential Mycoherbicide Agent

Cited by 63 publications

References 19 publications

Evaluation of a Fungal Strain, Myrothecium roridum F0252, as a Bioherbicide Agent

Evaluation of a Fungal Strain, Myrothecium roridum F0252, as a Bioherbicide Agent

Extending the Shelf-Life of &lt;i&gt;Myrothecium verrucaria&lt;/i&gt;, a Bioherbicide

Bioherbicides: Research and Risks

Contact Info

Product

Resources

About

Extending the Shelf-Life of <i>Myrothecium verrucaria</i>, a Bioherbicide