Evaluation of an isotope dilution liquid chromatography tandem mass spectrometry method for pharmaceuticals in fish

Du, Bowen; Perez‐Hurtado, Pilar; Brooks, Bryan W.; Chambliss, C. Kevin

doi:10.1016/j.chroma.2012.07.026

Cited by 86 publications

(78 citation statements)

References 28 publications

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“…Instrumentation parameters, separation strategy, detection of target analytes, calibration method and method detection limits (MDLs) generally followed previously reported methods [20]. In this study, MDLs represented the lowest concentrations of an analyte that were reported with 99% confidence that the concentration is different from zero in a given matrix.…”

Section: Materials and Methods (A) Study Site And Field Samplingmentioning

confidence: 99%

“…Plasma sample extraction followed a slightly modified methodology [24]. Similarly, this tissue sample extraction protocol generally followed previously developed methodologies [8,20]. Detailed information regarding mass, length and composite groupings is provided in the electronic supplementary material, table S2.…”

Section: Materials and Methods (A) Study Site And Field Samplingmentioning

confidence: 99%

“…(b) Chemicals, regents and sample analysis Selection of 23 target analytes was based on previously reported occurrences of these pharmaceuticals in aquatic ecosystems [20,21]. All chemicals and their corresponding isotopically labelled analogues were obtained with various vendors [20,21] and used as received (see the electronic supplementary material, table S1).…”

Section: Materials and Methods (A) Study Site And Field Samplingmentioning

confidence: 99%

“…All chemicals and their corresponding isotopically labelled analogues were obtained with various vendors [20,21] and used as received (see the electronic supplementary material, table S1). Sample extraction and analysis followed previous methods [21 -23] in which isotope dilution was used to alleviate matrix interference with isotopically labelled internal standards for each target analyte.…”

Section: Materials and Methods (A) Study Site And Field Samplingmentioning

confidence: 99%

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Bioaccumulation and trophic dilution of human pharmaceuticals across trophic positions of an effluent-dependent wadeable stream

Haddad

Luek

et al. 2014

Phil. Trans. R. Soc. B

133

118

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Though pharmaceuticals are increasingly observed in a variety of organisms from coastal and inland aquatic systems, trophic transfer of pharmaceuticals in aquatic food webs have not been reported. In this study, bioaccumulation of select pharmaceuticals was investigated in a lower order effluent-dependent stream in central Texas, USA, using isotope dilution liquid chromatography–tandem mass spectrometry (MS). A fish plasma model, initially developed from laboratory studies, was tested to examine observed versus predicted internal dose of select pharmaceuticals. Pharmaceuticals accumulated to higher concentrations in invertebrates relative to fish; elevated concentrations of the antidepressant sertraline and its primary metabolite desmethylsertraline were observed in the Asian clam, Corbicula fluminea , and two unionid mussel species. Trophic positions were determined from stable isotopes (δ 15 N and δ 13 C) collected by isotope ratio-MS; a Bayesian mixing model was then used to estimate diet contributions towards top fish predators. Because diphenhydramine and carbamazepine were the only target compounds detected in all species examined, trophic magnification factors (TMFs) were derived to evaluate potential trophic transfer of both compounds. TMFs for diphenhydramine (0.38) and carbamazepine (1.17) indicated neither compound experienced trophic magnification, which suggests that inhalational and not dietary exposure represented the primary route of uptake by fish in this effluent-dependent stream.

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Section: Materials and Methods (A) Study Site And Field Samplingmentioning

confidence: 99%

Section: Materials and Methods (A) Study Site And Field Samplingmentioning

confidence: 99%

Section: Materials and Methods (A) Study Site And Field Samplingmentioning

confidence: 99%

Section: Materials and Methods (A) Study Site And Field Samplingmentioning

confidence: 99%

See 2 more Smart Citations

Bioaccumulation and trophic dilution of human pharmaceuticals across trophic positions of an effluent-dependent wadeable stream

Haddad

Luek

et al. 2014

Phil. Trans. R. Soc. B

133

118

View full text Add to dashboard Cite

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“…A 500-μL aliquot of undiluted or diluted exposure water samples was combined with 450 μL of 0.1% formic acid (w/w) and spiked with 50 μL of an internal standard mixture (DPH-d 3 and DZN-d 10 ) in an autosampler vial before analyses. Instrumentation and strategies for separation and detection of target analytes were primarily described elsewhere (25,26 …”

Section: Chemicals and Analytical Verification Of Treatment Levelsmentioning

confidence: 99%

Comparative Pharmacology and Toxicology of Pharmaceuticals in the Environment: Diphenhydramine Protection of Diazinon Toxicity in Danio rerio but Not Daphnia magna

Kristofco

Chambliss

et al. 2014

AAPS J

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Abstract. Pharmaceuticals and other contaminants of emerging concern present unique challenges to environmental risk assessment and management. Fortunately, mammalian pharmacology and toxicology safety data are more readily available for pharmaceuticals than other environmental contaminants. Identifying approaches to read-across such pharmaceutical safety information to non-target species represents a major research need to assess environmental hazards. Here, we tested a biological readacross hypothesis from emergency medicine with common aquatic invertebrate and vertebrate models. In mammals, the antihistamine diphenhydramine (DPH) confers protection from poisoning by acetylcholinesterase inhibition because DPH blocks the acetylcholine receptor. We employed standardized toxicity methods to examine individual and mixture toxicity of DPH and the acetylcholinesterase inhibitor diazinon (DZN) in Daphnia magna (an invertebrate) and Danio rerio (zebrafish, a vertebrate). Though the standardized Fish Embryo Toxicity method evaluates early life stage toxicity of zebrafish (0-3 days post fertilization, dpf), we further evaluated DPH, DZN, and their equipotent mixture during three development stages (0-3, 3-6, 7-10 dpf) in zebrafish embryos. Independent action and concentration addition mixture models and fish plasma modeling were used to assist interpretation of mixture toxicity experiments. Though our primary hypothesis was not confirmed in acute studies with Daphnia magna, DPH conferred a protective effect for acute DZN toxicity to zebrafish when DPH plasma levels were expected to be greater than mammalian therapeutic, but lower than acutely lethal, internal doses. We further observed that timing of developmental exposure influenced the magnitude of DZN and DPH toxicity to zebrafish, which suggests that future zebrafish toxicity studies with pharmaceuticals and pesticides should examine exposure during developmental stages.KEY WORDS: alternative toxicity test methods; biological read-across; comparative pharmacology and toxicology; mixture toxicology; pharmaceuticals in the environment.

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A fast and accurate isotope dilution GC‐IT‐MS/MS method for determination of eugenol in different tissues of fish: Application to a depletion study in mandarin fish

Liu

et al. 2018

Biomedical Chromatography

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An accurate, rapid and effective method was established for determination of eugenol in plasma, muscle, skin, liver, kidney and gill of fish using gas chromatography-ion trap tandem mass spectrometry. Samples of muscle, skin, liver, kidney and gill were prepared using the modified QuEChERS (quick, easy, cheap, effective, rugged and safe) procedure, and a plasma sample was prepared by a liquid-liquid extraction procedure. Eugenol was monitored in <7 min using an electron-ionization source in MS/MS mode and quantified by an internal standard of eugenol-d . The limit of detection was 5.0 μg/kg, and the limit of quantification was 10.0 μg/kg. The calibration curve was linear in the range of 5-1000 μg/L (R = 0.9996). Intra- and inter-day precisions of eugenol expressed as relative standard deviation were within 9.74%, and the accuracy exhibited a relative error ranging from -2.20 to 8.89%. The developed method was successfully used to study the elimination regularity of eugenol in mandarin fish.

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Evaluation of an isotope dilution liquid chromatography tandem mass spectrometry method for pharmaceuticals in fish

Cited by 86 publications

References 28 publications

Bioaccumulation and trophic dilution of human pharmaceuticals across trophic positions of an effluent-dependent wadeable stream

Bioaccumulation and trophic dilution of human pharmaceuticals across trophic positions of an effluent-dependent wadeable stream

Comparative Pharmacology and Toxicology of Pharmaceuticals in the Environment: Diphenhydramine Protection of Diazinon Toxicity in Danio rerio but Not Daphnia magna

A fast and accurate isotope dilution GC‐IT‐MS/MS method for determination of eugenol in different tissues of fish: Application to a depletion study in mandarin fish

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