Evaluation of an optimal temperature for brain storage in delayed 2,3,5-triphenyltetrazolium chloride staining

Infarct volume quantification in mouse focal cerebral ischemia: a comparison of triphenyltetrazolium chloride and cresyl violet staining techniques

“…Previously, TTC staining was confirmed by comparison to other histological staining methods to determine the infarct area (Park et al, 1988;Hatfield et al, 1991;Okuno et al, 2001). The limitations of the TTC technique seem to be the incubation time, incubation temperature, the effect of macrophage accumulation in the infarction and differences in tissue preparation techniques such as perfusion or immersion (Liszczak et al, 1984;Park et al, 1988;Hatfield et al, 1991;Dettmers et al, 1994;Li et al, 1997;Khan et al, 2000). In fact, TTC staining does not necessarily require heating to be performed, but it does significantly speed up the reaction.…”

PAR-1 Deficiency Protects against Neuronal Damage and Neurologic Deficits after Unilateral Cerebral Hypoxia/Ischemia

“…After a survival period of 2 days, all mice were euthanized and infarct volume was assessed via staining with 2% TTC (Sigma-Aldrich, St. Louis, MO) staining in 2-mm-thick coronal brain sections (Khan et al, 2000). TTC is reduced by succinate dehydrogenase to form a red formazan product in viable tissue; healthy tissue is stained red and damaged tissue is unstained and remains white.…”

Characterization of cellular and neurological damage following unilateral hypoxia/ischemia

“…For TTC staining, brains were immediately removed, cut into 1-mm sections, and stained with 2% 2,3,5-triphenyltetrazolium chloride (TTC) (Sigma-Aldrich, St. Louis, MO) and postfixed with 4% paraformaldehyde [9]. For all of the other histological protocols, mice were transcardially perfused with 4% paraformaldehyde.…”