Evaluation of anaerobic germinability in various rice subpopulations: identifying genotypes suitable for direct-seeded rice cultivation

Rauf, Muhammad; Choi, Yu-Mi; Lee, Sukyeung; Lee, Myung Chul; Oh, Sejong; Hyun, Do Yoon

doi:10.1007/s10681-019-2341-5

Cited by 15 publications

(10 citation statements)

References 46 publications

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“…The relationship between other differentially expressed miRNAs during the seed aging process remains to be elucidated (Table ). Multiple factors including enzymes and solutes have been identified as key determinants of seed vigor in various plant species, such as isopropylmalate synthase1 (IPMS1) in rice (He et al ), OVP3 and RAmy3D under anaerobic conditions in rice (Rauf et al ), raffinose family oligo saccharides in maize (Li et al ), and DNA LIGASE VI in Arabidopsis (Waterworth et al ). Therefore, whether miR164c and miR168a act synergistically on these enzymes, and whether these miRNAs interact with other miRNAs or genes to regulate seed vigor should be investigated.…”

Section: Discussionmentioning

confidence: 99%

“…Levels of free amino acids and soluble sugars are significantly higher in IIYou998 seeds with low life span during storage (Gao et al ; Yan et al ). The proton pyrophosphatase locus OVP3 and rice alpha amylase locus RAmy3D show distinct expression patterns among diverse rice accessions with different germination capacity under anaerobic conditions, which is used as an index for the evaluation of rice germplasm suitable for direct seeded cultivation (Rauf et al ).…”

Section: Introductionmentioning

confidence: 99%

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miR164c and miR168a regulate seed vigor in rice

Zhou

Wang

et al. 2019

JIPB

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MicroRNAs (miRNAs) are key regulators of gene expression in many important biological processes of plants. However, few miRNAs have been shown to regulate seed vigor. Here, we conducted microarray assays to analyze miRNA expression levels in seeds of the rice (Oryza sativa L.) cultivar ZR02. Results showed significant differences in the expression of 11 miRNAs between artificially aged and untreated control seeds. Among these, osa‐miR164c was transcriptionally upregulated, while osa‐miR168a was downregulated in artificially aged seeds; this was verified by quantitative real‐time PCR analysis. Under the same aging condition, osa‐miR164c overexpression in OE164c transgenic seeds and osa‐miR168a silencing in MIM168a transgenic seeds of the rice cultivar Kasalath led to lower germination rates, whereas osa‐miR164c silencing in MIM164c and osa‐miR168a overexpression in OE168a resulted in higher seed germination rates compared with wild‐type seeds. Meanwhile, changes in cytomembrane permeability of seeds and in the expression level of osa‐miR164c target genes (OsPM27 and OsPSK5) and osa‐miR168a target genes (OsAGO1 and OsPTR2) under aging conditions coincided with changes in seed vigor induced by osa‐miR164c and osa‐miR168a. Thus, genetic manipulation of miRNAs has important implications in the development of crop cultivars with high vigor and extended life span of seeds.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

miR164c and miR168a regulate seed vigor in rice

Zhou

Wang

et al. 2019

JIPB

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“…Ubiquitin was used as the endogenous reference gene to normalize the data. The relative expression of the scavenging genes (primer details are given in Supplementary Table 1 ) was estimated using 2 −Δ ΔCT method (Livak and Schmittgen, 2001 ; Yoo et al, 2017 ; Çelik et al, 2019 ; Rauf et al, 2019 ).…”

Section: Experiments 1: Stress Imposition At Seedling Stage In Contrasmentioning

confidence: 99%

Induction of Acquired Tolerance Through Gradual Progression of Drought Is the Key for Maintenance of Spikelet Fertility and Yield in Rice Under Semi-irrigated Aerobic Conditions

et al. 2021

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Plants have evolved several adaptive mechanisms to cope with water-limited conditions. While most of them are through constitutive traits, certain “acquired tolerance” traits also provide significant improvement in drought adaptation. Most abiotic stresses, especially drought, show a gradual progression of stress and hence provide an opportunity to upregulate specific protective mechanisms collectively referred to as “acquired tolerance” traits. Here, we demonstrate a significant genetic variability in acquired tolerance traits among rice germplasm accessions after standardizing a novel gradual stress progress protocol. Two contrasting genotypes, BPT 5204 (drought susceptible) and AC 39000 (tolerant), were used to standardize methodology for capturing acquired tolerance traits at seedling phase. Seedlings exposed to gradual progression of stress showed higher recovery with low free radical accumulation in both the genotypes compared to rapid stress. Further, the gradual stress progression protocol was used to examine the role of acquired tolerance at flowering phase using a set of 17 diverse rice genotypes. Significant diversity in free radical production and scavenging was observed among these genotypes. Association of these parameters with yield attributes showed that genotypes that managed free radical levels in cells were able to maintain high spikelet fertility and hence yield under stress. This study, besides emphasizing the importance of acquired tolerance, explains a high throughput phenotyping approach that significantly overcomes methodological constraints in assessing genetic variability in this important drought adaptive mechanism.

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“…Indeed, no correlation between amylase activity in endosperm and coleoptiles under anoxia was found by Magneschi and Perata 2009, while high sugar content in embryo was found to be positively correlated to total amylolytic activities under anaerobic condition (Pompeiano et al 2013). Rauf et al 2019, has also reported that high Ramy3D mRNA accumulation in tolerant accessions suggests that major genes involved in anaerobic germination may vary depending on germplasm.…”

Section: Discussionmentioning

confidence: 97%

Identification of novel hypoxia-responsive factors in deep-water rice conferring tolerance to flood during germination

Kumar¹,

Das²,

Sarmah³

2020

Biol. plant.

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Flood in rice fields at the time of seed sowing and early seedling establishment causes extensive crop loss due to the inability of the seeds to tolerate and overcome submergence. In the present study, rice genotypes from Assam, India tolerant to hypoxia during germination were identified through systematic screening of deep-water rice genotype collection from flood-prone ecosystem of Brahmaputra river valley. The difference in tolerance to hypoxia during germination within the species provides scope for identification of novel hypoxia-responsive factors involved in tolerance as mechanism of tolerance may not be conserved across tolerant germplasms. Tolerant genotypes were further subjected to physiological and molecular characterization. Growth rate kinetics in tolerant genotypes new Rangdhakekua bao (RKB) and cultivar Khao Hlan On (KHO) from International Rice Research Institute (IRRI) used as positive control exhibited stronger escape strategy under hypoxic condition compared to sensitive genotype IR-64 (negative control). Activities of α-amylase and pyruvate decarboxylase were significantly higher in RKB and KHO than in IR-64, while no significant difference was observed in the alcohol dehydrogenase activity. Reverse transcription quantitative PCR confirmed increased amounts of transcripts of sucrose nonfermenting 1 related protein kinase, myeloblastosis-related protein S1, rice amylase 3D, and trehalose phosphate phosphatase 7 genes, which are known to be involved in hypoxia signaling cascade. Besides, transcription factors (TFs) like ethylene responce factors 71 and 63, ethylene insensitive 3-like 1a and proteins like expansins A7 and A2, which are involved in cell elongation had also significantly higher amounts in RKB compared to IR-64. Additional factors that include TFs like ERF71 and ERF63 that shows perturbation at transcription even within tolerant genotypes might constitute the genotype-specific regulation, evolved as a part of its adaptive mechanism to survive under submerged conditions.

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Evaluation of anaerobic germinability in various rice subpopulations: identifying genotypes suitable for direct-seeded rice cultivation

Cited by 15 publications

References 46 publications

miR164c and miR168a regulate seed vigor in rice

miR164c and miR168a regulate seed vigor in rice

Induction of Acquired Tolerance Through Gradual Progression of Drought Is the Key for Maintenance of Spikelet Fertility and Yield in Rice Under Semi-irrigated Aerobic Conditions

Identification of novel hypoxia-responsive factors in deep-water rice conferring tolerance to flood during germination

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