Evaluation of anthelmintic potential of Parkia biglobosa leaves and seeds extracts against infective larvae and adult of Haemonchus contortus of goats

Abstract

Among the parasitic diseases that thrive in warm and humid areas, Haemonchus contortus is considered to be the most prevalent and devastating species of small ruminant. The present study was carried out to evaluate the activities of leaves and seeds of Parkia biglobosa against adult and larvae (L3) of H. contortus. In vitro activities were screened by preparing aqueous and methanol extracts of both leaves and seeds of P. biglobosa in comparable to controls. Ten worms were exposed to treatment of each plant extract and Albendazole in separate Petri-dishes with 2, 4, 8, 16 and 32 mg/ml of the same volume. The negative control received Phosphate Buffered Saline (PBS). For infective larvae, 20 L3 were pipetted into 96-flat-bottomed microtitre plate and mixed with the same volume of different concentrations similar to that of adult. The result revealed that, all the adult worms exposed to 32 mg/ml of Albendazole and plant extracts were found dead at 12 hours; whereas none of the worms was found dead in PBS up to 12 hours post exposure. The result of larvae showed that the leaves and seeds extracts of P. biglobosa exposed to L3 of H. contortus exhibited less anthelmintic effects in comparable to Albendazole. Albendazole exhibited anthelmintic effects in a dose dependent manner and about 88.5% of L3 were found dead at 12 hours post-exposure at concentration of 32 mg /ml. But for plant extracts, at 32 mg/ml, less than 40% of L3 were found dead at 12 hours post exposure. All L3 larvae survived in PBS up till 12 hours post exposure. It is therefore, concluded that, 32 mg/ml of aqueous and methanol extracts of leaves and seed of P. biglobosa have higher adulticidal activity at 12 hours post exposure but lower larvicidal activity against H. contortus. However, it is recommended to carry out the in vivo study to assess the toxicological effect and recommended doses in goats.

Acute toxicity profile of crude methanolic stem bark extract of Parkia biglobosa in West African Dwarf (WAD) goats

“…The results in this study were similar to that of Ezekwe et al (2013) and Josiah et al (2022) in methanol extracts of stem bark of P. biglobosa. Millogo-Kone et al (2006) also reported the presence of saponins, glycosides, tannins and other phenolics with trace quantity of alkaloids while Banwo et al (2004) confirmed the same.…”

Evaluation of the Inhibitive Properties of Silver Nanoparticles in Senna occidentalis Root Extract as Corrosion Inhibitor of Mild Steel

“…The higher values of E a in the presence of an inhibitor were due to the increased energy barrier. This result suggests that the corrosion inhibition by silver nanoparticles obtained from Senna occidentalis is feasible because of the increase in energy barrier for the metal dissolution (Josiah et al 2023).…”

In vitro anthelmintic activities of stem and root barks extracts of Parkia biglobosa on infective larvae and adult of Haemonchus contortus

“…Several researchers (Ademola et al, 2005;Bizimenyera et al, 2006;Soetan et al, 2011) have reported the activities of in vitro anthelmintic study of plant extracts for the treatment of gastro-intestinal helminths of animals. Therefore, perturbation induced by anthelmintic plants on infective larvae and adult worm survival or their prolificacy that constitute the pathogenic stage could be an important element in parasites struggle (Josiah et al, 2018).…”