Evaluation of Anti-Anxiety Effect of Brassica oleracea L. Extracts in Experimental Animals

Abstract

Introduction:Oxidative stress in the brain causes nervous system impairment. Hence the use of antioxidants could be an effective approach to prevent disorders such as anxiety. Plants containing phenolic constituents are reported to be potent antioxidants. Brassica oleracea L. commonly known as broccoli, is rich in phenolics mainly flavonoids and possesses excellent antioxidant property, however its anxiolytic potential has not been investigated. Thus in the present study antianxiety activity of extracts of broccoli was evaluated in experimental animals. Materials and Methods: Various pharmacognostic parameters of the plant material were studied following standard procedures. Groups of six mice each were taken as different treatment groups. Control group received vehicle (1% Carboxy Methyl Cellulose), positive control received Diazepam (2mg/kg) as standard drug while test groups received petroleum ether and hydroalcoholic extracts (50, 100 and 200 mg/kg body weight, p.o.). The effects were evaluated on Elevated Plus Maze (EPM), hole board and mirror chamber tests. Statistical analysis was performed using ANOVA, followed by Turkey's post-hoc test. Results: Hydroalcoholic extract shows dose dependent increase in the average time spent and frequency of entries in the open arms of the EPM; decreased latency, increased time spent and frequency of entries in the mirror chamber; increased number of head dips in hole board test as compared to control was observed. These effects were comparable to the effect produced by diazepam. Phytochemical screening of the bioactive extract revealed the presence of alkaloids, phenols, flavonoids and tannins. Conclusion: From our study, it can be concluded that hydroalcoholic extract of Brassica oleracea at the dose of 200 mg/kg possesses marked anxiolytic activity.

Evaluation of the effectiveness of macaíba palm seed kernel (Acrocomia intumescens drude) on anxiolytic activity, memory preservation and oxidative stress in the brain of dyslipidemic rats

“…An increase in oxidative stress could induce abnormalities and changes in membrane lipids and proteins [ 49 ]. Anxiety, as well as other neurobehavioral changes, can be mediated by oxidative stress damages in the brain [ 50 ]. Oxidative stress is an important factor in development of neurodegenerative and neuropsychiatric diseases, including stress and anxiety [ 51 ].…”

Protective effect of Grewia asiatica leaves extract in animal models of epilepsy and anxiety

“…In the mirror chamber model, mice were placed individually in the chamber of mirrors at a fixed corner. During the five minute test period latency to enter the chamber, the number of entries and the time spent with each entry in the mirrored chamber was recorded [ 16 , 17 ].…”

Bioactivity‐directed isolation, characterization, and quantification of an anxiolytic flavonoid from Brassica oleracea L.

“…Hydroalcoholic (HA) extract was dried using rotary evaporator at 40°C and freeze dried in a lyophilizer and then, kept in a vacuum desiccator. The yield was calculated on dry weight basis (Kaur et al., 2017) which was 18.75 g. It was 2.5% w/w on dry weight basis.…”

“…In our earlier investigation, hydroalcoholic extract of Brassica oleracea at the dose of 200 mg/kg exhibited marked anxiolytic activity assessed using elevated plus maze model (Kaur et al., 2017). In the present study, this extract was subjected to bioactivity‐directed fractionation using column chromatography and preparative TLC to separate the bioactive constituent responsible for the anxiolytic effect of this plant.…”