Evaluation of Anti-diarrheal and Anti-nociceptive Activity of Methanolic Unripe Fruit Peels Extract of Masua ferrea Linn. on Mice Models

Abstract

and Thailand. Mature leaves (7-15 cm long) of Mesua ferra are blue grey to dark green in color, whereas, young leaves appear as reddish yellow. In flowering season, it produces large fragranted flowers with four petals with numerous yellowish stamens at the centre, whereas, fruits are sometimes beaked with slight woody appearance and contains 1-4 seeds. Bark appears as reddish brown and flower, fruit, seeds and leaves of this plant are edible. 9 Traditionally, different parts of this plant is used as antipyretic, antimicrobial, anticancer, cardiotonic, diuretic, expectorant, poultice, aroma, wound healer, bleeding piles, excessive thirst, itching, sweating, ABSTRACT Background: Though traditionally the plant Masua ferrea has been used by south east Asian people in inflammation and septic conditions, the present study was designed to investigate the anti-diarrheal and anti-nociceptive activities of unripe fruit peel of Mesua ferrea on animal models. Methods: Various methods were employed for investigating these activities such as castor-oil induced diarrhea, castor-oil induced enteropooling and gastrointestinal motility test, acetic acid induced writhing test, tail immersion and hot plate methods. Results: From the experimental data it was found that the diarrheal episode was inhibited by 39.68% and 49.21% for methanol extract at the doses of 100 and 200 mg/kg respectively. The extract significantly lessened the intestinal volume for methanolic extract at 200 mg/Kg dose 0.54 ± 0.01 ml (p < 0.05) and at 200 mg/Kg dose 0.47 ± 0.02 ml (p < 0.01) compared to control 0.65 ± 0.03 ml in castor-oil induced enteropooling and also decreased intestinal transit 29.07-48.54% for methanolic extract comparable with standard (loperamide 5 mg/kg). Mesua ferrea peels significantly (P < 0.05, P < 0.01 and P < 0.001) reduced the number of writhing, increased latency to flick tail in tail immersion method and elevated the mean basal reaction time in hot plate method respectively. Besides, no delayed toxicity was observed in given doses. Conclusion: The methanolic extract exhibited highly significant anti-diarrheal and antinociceptive activity in a dose-dependent manner, which supports its use in traditional herbal medicine.

Evaluation of Various Solvent Extracts of Tetrastigma leucostaphylum (Dennst.) Alston Leaves, a Bangladeshi Traditional Medicine Used for the Treatment of Diarrhea

“…The ADI value is an indication of the efficiency of an extract in the management of diarrhea [26]. In our study, the ADI value increased with increasing dose of the extracts, suggesting a dose response relationship with this parameter.…”

Evaluation of Antidiarrhoeal Activity of the Leaves and Stem of Bauhinia vahlii

“…Animals of Group I (control group) was given normal saline (2ml/kg) orally while Group II (standard group) received standard drug loperamide (5 mg/kg). 18 The rest of the groups III and IV were given EEBVL in dose of 200 and 400 mg/kg bw whereas group V and VI were given EEBVS 200 and 400 mg/kg bw respectively. After 1 h, all groups received castor oil, 1ml orally per animal.…”