Evaluation of Anti-hypertrophic Potential of Enicostemma littorale Blume on Isoproterenol Induced Cardiac Hypertrophy

Doss, Victor Arokia; Kuberapandian, Dharaniyambigai

doi:10.1007/s12291-019-0814-x

Cited by 10 publications

(6 citation statements)

References 29 publications

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“…ISO elevates glucose, protein and cholesterol levels by impairing insulin activity and AMPK activities. Decreased albumin (hypoalbuminemia), considered as risk factors for cardiac hypertrophy , is observed in ISO, which could be attributed to the inhibitory effects of ISO upon albumin (Doss and Kuberapandian, 2019). Similarly, in this study, raised glucose, protein and cholesterol levels and decreased albumin (hypoalbuminemia), as shown in Table 2, were observed in the hypertrophic groups were reduced after treatment with the plant extract and losartan.…”

Section: Effect Of P Betle On Glucose Total Protein and Total Cholesupporting

confidence: 76%

“…Similar results were observed in most studies, but LDH in a recent study was reported with decreased levels in ISO treated groups due to metabolic alterations of AMPK. So, the relationship between AMPK-LDH warrants further investigation in terms of CH (Doss and Kuberapandian, 2019;Long, 2006;Doss et al, 2018). Cardiac markers enzymes, namely SGOT, SGPT, LDH, were increased in serum of isoproterenol induced rats and were reduced by the administration of aqueous (juice) extract of P. betle as shown in Table 3.…”

Section: Effect Of P Betle On Serum Cardiac Marker Enzymesmentioning

confidence: 99%

“…Free radicals trigger the cellular stress underlying the metabolic and cellular alterations involved in the pathophysiology of CH (Widowati et al, 2013;Maulik and Kumar, 2012). ISO being cardiotoxic induces oxidative stress marked by the decreased levels of enzymic antioxidants, which upon treatment using P.betle is involved in the restoration of SOD, CAT and GPx when compared to ISO administered rats (Doss and Kuberapandian, 2019;Wexler, 1979;Wu et al, 2009). Decreased enzymic antioxidants (SOD, catalase, GPx) and increased level of lipid peroxidation were seen in the isoproterenol induced rat models.…”

Section: Effect Of P Betle On Enzymic Antioxidantsmentioning

confidence: 99%

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Cardioprotective activity of Piper betle juice in isoproterenol induced hypertrophic rat models

Doss¹,

Arumugam²,

Kuberapandian³

2020

ijrps

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Impaired electrolytes (preferably Na+, K+, Cl-, HCO3-) is one of the key fundamental complications associated with cardiac hypertrophy (enlarged heart) mediated heart failure. Piper betle (P. betle - family Piperaceae) due to its potent antioxidant activity is used in various pharmacological applications. The present study evaluates the cardioprotective (anti-hypertrophic) potential of aqueous (juice) extract of P. betle in restoring electrolyte homeostasis, thereby its ability to curb the fundamental progression of hypertrophy. Isoproterenol (ISO - 10 mg/kg b.w., i.p., 7 days) induced cardiac hypertrophic rats were developed that were simultaneously treated with the standard drug losartan (50mg/kg b.w., orally., 7days) and aqueous juice extract of P. betle (30mg/kg b.w., orally., 7 days). The biochemical estimation of glucose, protein, cholesterol, cardiac marker enzymes like SGOT, SGPT, LDH, enzymatic antioxidants namely SOD, catalase, GPx and electrolytes (Na+, K+, Cl- and bicarbonate) in serum were statistically performed along with the histopathological analysis of left ventricles (H&E stain). The present study showed increased levels of glucose, protein, cholesterol, cardiac marker enzymes, reduced enzymatic antioxidants with reduced electrolytes during cardiac hypertrophy (ISO) that were reflected in a histopathological analysis by thickened abnormal myocardial architecture which was reversed similar to normal in plant juice administered rats. Hence, this study demonstrated the beneficial effect of P. betle (aqueous juice) on electrolytes (Na+, K+, Cl-, HCO3-), thereby imposing an interventional effect against cardiac hypertrophy.

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Section: Effect Of P Betle On Glucose Total Protein and Total Cholesupporting

confidence: 76%

Section: Effect Of P Betle On Serum Cardiac Marker Enzymesmentioning

confidence: 99%

Section: Effect Of P Betle On Enzymic Antioxidantsmentioning

confidence: 99%

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Cardioprotective activity of Piper betle juice in isoproterenol induced hypertrophic rat models

Doss¹,

Arumugam²,

Kuberapandian³

2020

ijrps

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“…After oral administration of the combination extract of P. betle, there was a significant decrease in the levels of glucose, total protein, and total cholesterol when compared with Group II and these levels restored to the normal range as shown in Table 4. These results indicate the potential cardioprotective effect of the plant extract [26].…”

Section: Effect Of Iso and Plant Extract On Glucose Total Protein Asupporting

confidence: 51%

Evaluation of Anti-Hypertrophic Potential of Piper Betle in Isoproterenol-Induced Cardiac Hypertrophic Rat Models

Doss¹,

Sakthikumar²,

Kuberapandian³

2019

Asian J Pharm Clin Res

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Objective: The objective of this study was to evaluate the anti-hypertrophic potential of the combination extract (chloroform, methanol, and ethyl acetate) of Piper betle in cardiac hypertrophic rat models. Methods: Isoproterenol (ISO) (10 mg/kg b. w., i.p., 7 days) was used to induce cardiac hypertrophy in male albino Wistar rats and simultaneously treated with the combination extract of P. betle (50 mg/kg b. w., oral, 7 days) and the standard drug losartan (50 mg/kg b. w., oral, 7 days) as reference. At the end of seventh day the biochemical estimations of glucose, protein, cholesterol, triglycerides, cardiac marker enzymes (SGOT, SGPT and LDH) and enzymic antioxidants (superoxide dismutase, catalase and glutathione peroxidase) were performed in the serum along with the targets of the study namely calcium and phosphorus. Heart tissues were subjected to histopathological analysis. All biochemical assays were statistically verified. Results: The status of cardiac hypertrophy was indicated by the increased heart weight (HW)/body weight (BW) ratio. The biochemical assays showed significant (p<0.05) increase in the levels of glucose, protein, and cholesterol and in the cardiac marker enzymes such as SGOT, SGPT, and LDH, whereas significant decrease in the serum calcium and phosphorus along with antioxidants were seen in the ISO-induced rats and these levels were restored to normal values by the treatment with P. betle. Conclusion: The combination extract of P. betle showed effect upon calcium and phosphorus levels besides reversing the other impacts of isoproterenol (β-adrenergic hypersignaling events) probably due to phytoconstituents of the plant which are hypothesized to be efficiently extracted in combination of organic solvents, and thus, these results indicate that the combination extract of P. betle possesses cardioprotective (anti-hypertrophic) effect.

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“…In each group, Cardiac hypertrophy was induced to albino wistar rats using isoproterenol and simultaneous treatment using the hydroethanolic plant extract and the reference drug, and losartan was done, as shown in Table 1,Table 2 shows, After the end of the experimental treatment period (7 days), the grouped animals were sacri iced under mild anaesthesia. Blood was collected by cardiac puncture method, and the serum sample was separated by centrifugation at 5000 rpm for 20 min (Doss and Kuberapandian, 2019) . Then, immediately, the heart tissues were excised for histopathological analysis.…”

Section: Experimental Groupsmentioning

confidence: 99%

Estimation rate of S. aureus and MRSA carriage in diabetic type-2 and effect of Aspergillus Gliotoxin on bacterial carriage in type-2 diabetes

Al-aajem¹

2018

Int. J. Res. Ph. Sci.

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Diabetes mellitus is a serious public health problem, S. aureus and MRSA are the most common bacteria isolated from ulceration of diabetic patients. The aim of the study was to estimation rate of S. aureus and MRSA carrier in diabetes type-2 and determine the antimicrobial effect of Gliotoxin on the previous bacterial carriage in type-2 diabetes. The study was conducted on 450 diabetics' patients, attended the outpatients clinic in Baquba Teaching Hospital, their ages ranged from 15-65years, with mean age of 36.15, and 150 healthy group, who were randomly selected, during the period from May 2016 to April 2017, patients were classified into two groups according to the type of diabetes, group1 included: 184 with type1-diabetes, and group2: included 266 with type2-diabetes, 97 patients with foot ulcers. Swabs were taken from anterior nares, toe and axillae for each diabetic patient type-2, identified based on standard bacteriological methods. Using the Kirby -Bauer method for detection the antibacterial effect of Gliotoxin. The results showed rates of the bacterial carriage in anterior nares of type-2diabetic patients without complications were (11.4%), (4.4%), respectively for S. aureus and MRSA, in type-2 diabetes with complications were (8.6%), (2.1%) respectively for S. aureus and MRSA. In the toe of type-2diabetic patients without complications were (6.7%), (2.5%) respectively for S. aureus and MRSA. In with complications were (9.2%), (5%) respectively. In the axillae of diabetic patients, type-2 without complications was (5.5%), (4.5%) respectively. Aspergillus fumigatusGliotoxin was effective against bacterial carriage in diabetes type-2 with the foot ulcer, for S. aureus inhibition diameter was (20.50, 16.40, 12.20) mm for different concentrations of Gliotoxin, to MRSA was (8.25, 6.1, 4.20) mm. Increasing rate of S. aureus and MRSA carrier in diabetic patient's type-2 which lead to a significantly increased risk of bacterial infections. Gliotoxin was effective as an antibacterial agent against S. aureus and MRSA in type-2diabetis with the foot ulcer.

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Evaluation of Anti-hypertrophic Potential of Enicostemma littorale Blume on Isoproterenol Induced Cardiac Hypertrophy

Cited by 10 publications

References 29 publications

Cardioprotective activity of Piper betle juice in isoproterenol induced hypertrophic rat models

Cardioprotective activity of Piper betle juice in isoproterenol induced hypertrophic rat models

Evaluation of Anti-Hypertrophic Potential of Piper Betle in Isoproterenol-Induced Cardiac Hypertrophic Rat Models

Estimation rate of S. aureus and MRSA carriage in diabetic type-2 and effect of Aspergillus Gliotoxin on bacterial carriage in type-2 diabetes

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