Evaluation of Anti-inflammatory, Antinociceptive, and Antipyretic Activities of Prunus persica var. nucipersica (Nectarine) Kernel

Abstract

Nectarine (Prunus persica var. nucipersica) is a worldwide appreciated edible subspecies, with a high nutritional value and benefits on human health due to its phenolic content. Despite the large consumption of the fruit, the potential use of its kernel is poorly studied. Herein, the potential pharmacological activities and the phenolic constituents of an alcoholic extract of kernel nectarine fruits were investigated. Administering nectarine kernel extract (50 and 100 mg/kg, respectively) in rats reduced paw edema after carrageenan injection by 11 and 47% in 1 h, 24 and 33% in 2 h, and 23 and 32% in 4 h, when compared to the controls. At the higher dose (100 mg/kg), nectarine kernel extract increased the reaction time in the hot-plate model and produced a significant decrease in the rectal temperature of the pyretic rats, while both doses produced 52 and 59% of writhing inhibition compared to the control group. Total polyphenolic (55.91 ± 5.78 mg/g) and flavonoid (29.89 ± 0.55 mg/g) content indicated that the extract is a promising source of these constituents. HPLC-ESI-MS/MS analysis demonstrated the presence of flavonoids, such as naringenin and apigenin glycosides. The cyanogenic glycosides amigdalin and prunasin were also detected. These results highlight the anti-inflammatory, analgesic, and antipyretic activities of nectarine kernel alcoholic extract, together with significant phenolic content, promoting its exploitation as a source of bioactive molecules.

Protective Mechanism of Acacia saligna Butanol Extract and Its Nano-Formulations against Ulcerative Colitis in Rats as Revealed via Biochemical and Metabolomic Assays

“…Total polyphenols content, including total polyphenols (TPC) and total flavonoid contents (TFC), were determined according the previous described protocol [ 32 , 33 ]. The remain n -BuOH extract (15 g) was subjected to further fractionation over polyamide 6 L CC, eluted with a mixture of H 2 O/MeOH step gradient and afforded four main fractions (AS-1: AS-4) after final collection according to paper chromatography and TLC profile.…”

Protective Effects of Naringenin from Citrus sinensis (var. Valencia) Peels against CCl4-Induced Hepatic and Renal Injuries in Rats Assessed by Metabolomics, Histological and Biochemical Analyses

“…Recently, scientists have focused on the biological and pharmaceutical applications of metabolites derived from edible plants and foods [ 16 , 17 ]. Some reports have shown that naringenin and its glycosides exert significant hepatoprotective effects [ 18 , 19 , 20 ].…”

Extraction with Acidified Methanol—An Easy and Effective Method of Methyl Chlorogenate Formation, as Studied by ESI-MS

“…nucipersica (nectarine) are shown in the supplementary material (Figures S6–S8) . It is worth adding that the extract of nectarine kernels may have interesting biochemical properties as discussed in detail elsewhere [ 42 , 43 ].…”