Evaluation of Anti Proliferative Potential of Solanum virginianum Human Hepatocellular Carcinoma Cell Line

Abstract

Introduction: Solanum virginianum commonly known as wild eggplant or nightshade plant, is a prickly herb found in most of the parts of Asia and Australia of the world. It is used by the local people as folk medicines in treating throat infections and other inflammatory problems. Various phytoconstituents have been found, the major constituent is alkaloid. It has a vital role in various traditional as well as medicinal uses for curing internal and external physiological disorders. To determine the evaluation of anti proliferation potential of Solanum virginianum human hepatocellular carcinoma cell line.
 Materials and Methods: The effect of solanum virginianum hepG2 cell viability was measured by MTT assay. Briefly, the cells (1 × 104 cells per mL) were seeded in a 96 well microtiter plate (100 per well) with replications. Treatment was conducted for 24 hr with different concentrations (50, 100, 150, 200, 250, 300 μM) of solanum virginianum. Cell morphological changes were observed in phase contrast microscopy.
 Results: Cells were treated with solanum virginianum at higher concentration (50, 75, 100, 200, 300 and 400 μM) for 24 h, and cell viability was evaluated by MTT assay. Data are shown as means ± SD (n = 3). * compared with the control-blank group, p < 0.001.
 Conclusion: From the results, the extracts were cytotoxic to the hepG2 cells at (60μg/mL) concentration and incubation period. However more research is needed to understand the mechanisms of cytotoxicity of the plants.