Evaluation of anti-tumor effect of the exopolysaccharide from new cold-adapted yeast, Rhodotorula mucilaginosa sp. GUMS16 on chronic myeloid leukemia K562 cell line

Kheyrandish, Setare; Rastgar, Amirhossein; Hamidi, Masoud; Sajjadi, Seyed Mehdi; Sarab, Gholamreza Anani

doi:10.1016/j.ijbiomac.2022.02.113

Cited by 11 publications

(3 citation statements)

References 33 publications

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“…Furthermore, the DPPH scavenging activity exhibited by the EPS in the treated cells was found to be considerably higher than that in the control group. Te EPS derived from the GUMS16 strain has antioxidant capabilities and demonstrates the capacity to impede the growth of K562 cells [49].…”

Section: Probiotics Against Bloodmentioning

confidence: 99%

Role of Probiotics against Human Cancers, Inflammatory Diseases, and Other Complex Malignancies

Naeem,

Hassan,

Shahbaz

et al. 2024

Journal of Food Biochemistry

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Probiotics have growing medical importance as a result of their potential in the prevention and therapeutic support of several complex diseases, including different types of cancers. The anticarcinogenic properties of probiotics are attributed to various mechanisms, including alterations in the composition of the intestinal microbiota, suppression of cell proliferation, stimulation of apoptosis, inhibition of NF-kB, reduction in levels of H2AX, 8-hydroxy-deoxyguanosine, RIG-I, downregulation of IL-17, and TNF signaling pathway. Furthermore, probiotics have demonstrated significant advantages in the prevention and management of other complex diseases, including diabetes, obesity, and cardiovascular diseases. Probiotics had a considerable effect in reducing inflammatory infiltration and the occurrence of precancerous lesions. Additionally, the administration of probiotics led to a decrease in the appearance level of genes related to proinflammatory pathways, including NF-κB, IL-17, and TNF signaling pathways. However, further research studies are required to comprehend the processes via which probiotics exert their effects and to authenticate their potential as alternative therapeutic interventions.

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Section: Probiotics Against Bloodmentioning

confidence: 99%

Role of Probiotics against Human Cancers, Inflammatory Diseases, and Other Complex Malignancies

Naeem,

Hassan,

Shahbaz

et al. 2024

Journal of Food Biochemistry

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“…Primers were designed by Gene Runner version 3.05 (Hastings Software, Inc.) and sequences were analyzed using BLAST in GenBank data. The relative changes in the targeted genes were analyzed using the Livak method (2 −ΔΔCT ) method and normalized compared to the GAPDH expression level [33].…”

Section: Cell Cycle Analysis By Ow Cytometrymentioning

confidence: 99%

The Impact of Exosomes Derived from B-Cell Acute Lymphoblastic Leukemia as a Growth Factor on Bone Marrow Mesenchymal Stromal Cells

Amirpour,

Kuhestani,

Kheyrandish

et al. 2024

Preprint

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Background The incidence of various types of cancers, including leukemia, is on the rise and many challenges in both drug resistance and complications related to chemotherapy appeared. Recently, the development and application of extracellular vesicles (EV) such as exosomes in the management of cancers, especially leukemia, holds great significance. Aim In this article, we extracted exosomes from NALM6 cells and assessed their regulatory effects on proliferation and apoptosis in mesenchymal stem cells (MSCs). Method We first verified the exosomes using various techniques, including flow cytometry, transient electron microscopy, dynamic light scattering (DLS), and BCA protein assay. Then MTT analysis and flowcytometry (apoptosis and cell cycle assay) besides gene expressions were employed to determine the state of MSC proliferations. Result The results indicated that exosome-specific pan markers like CD9, CD63, and CD81 were present. Through DLS, we found out that the mean size of the exosomes was 89.68 nm. The protein content was determined to be 956.292 µg/ml. Analysis of MTT, flow cytometry (cell cycle and apoptosis assay), and RT-qPCR showed that in the dose of 50 µg/ml the proliferation of MSCs was increased significantly (p-value < 0.05). Conclusion All these data showed that exosomes use several signaling pathways to increase the MSCs’ proliferation and drug resistance, ultimately leading to high mortalities and morbidities of acute lymphoblastic leukemia.

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“…GUMS16 was reported to have an anti-tumor effect on chronic myeloid leukemia K562 cell line. The results suggested that apoptosis is induced in EPS-treated cells by an antiproliferative and growth-inhibitory effect ( Kheyrandish et al., 2022 ). EPS from Rhodotorula sp.…”

Section: Other Bio-properties Of R Muci...mentioning

confidence: 99%

Rhodotorula mucilaginosa—alternative sources of natural carotenoids, lipids, and enzymes for industrial use

Cheng

et al. 2022

Heliyon

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Evaluation of anti-tumor effect of the exopolysaccharide from new cold-adapted yeast, Rhodotorula mucilaginosa sp. GUMS16 on chronic myeloid leukemia K562 cell line

Cited by 11 publications

References 33 publications

Role of Probiotics against Human Cancers, Inflammatory Diseases, and Other Complex Malignancies

Role of Probiotics against Human Cancers, Inflammatory Diseases, and Other Complex Malignancies

The Impact of Exosomes Derived from B-Cell Acute Lymphoblastic Leukemia as a Growth Factor on Bone Marrow Mesenchymal Stromal Cells

Rhodotorula mucilaginosa—alternative sources of natural carotenoids, lipids, and enzymes for industrial use

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