Evaluation of antiasthmatic activity of ethanolic extract of Elephantopus scaber L. leaves

Abstract

Aims:The aim of study was to evaluate the scientific basis for the traditional use of Elephantopus scaber leaves.Materials and Methods:In the present study, ethanol extract of Elephantopus scaber leaves was evaluated for preliminary phytochemical screening and antiasthmatic activity using histamine and acetylcholine-induced bronchospasm, mast cell degranulation and histamine induced constriction on isolated guinea pig tracheal chain at different dose levels. Student's t-Test and Dunett's test were used for statistical analysis.Results:The result of present investigation showed that the ethanolic extract of E. scaber significantly (P<0.001) decreased the bronchospasm induced by histamine, acetylcholine and protected mast cell degranulation as compared to control groups. It also decreased the histamine induce constriction on isolated guinea pig trachea in dose-dependent manner. Phytochemical studies revealed the presence of steroids, saponin, flavonoids, and phenolic compounds in the extract.Conclusions:The present study concludes that the antiasthmatic activity of ethanolic extract of E. scaber leaves may be due to the presence of flavonoids or steroids. Antiasthmatic action of the E. scaber could be due to its antihistaminic, anticholinergic and mast-cell-stabilizing property.

Untitled

“…Further, there was more inhibition of histamine-induced ileum contractions supporting H1 receptor antagonistic activity than the antimuscarinic property of plant extracts. [43] GC-MS analysis reported the presence of major constituents, namely, methoxy-bis (cyclopentadiene), 5,10-dihexyl-5,10-dihydro-indolo [3,2-b]indole-2,7-dicarbaldehyde, and 1,2-bis[3,4-dimethoxy benzyl]-1,2-bis (methoxymethyl) ethane [ Table 5] and other constituents [ Table 6] which may be responsible for the therapeutic activity.…”

Pharmacological evaluation of calendula officinalis L. on bronchial asthma in various experimental animals

“…Once binding of allergen to cell-bound IgE occurs, the mast cell releases the inflammatory mediators such as histamine, eosinophils, and neutrophils chemotactic factors, leukotrienes, prostaglandins, platelet-activating factor, and so on; which are responsible development of airway inflammation and bronchoconstriction. [36] Here, an attempt was made to find out whether MECO has any effect on the rate of disruption of mast cells following exposure to compound 48/80, an agent which causes histamine release. In this study, MECO offered significant protection against compound 48/80-induced mast cell degranulation by stabilizing it, which is responsible for the decreasing airway inflammation by preventing release of various inflammatory mediators.…”

“…The percent response of each groups were calculated from the height of the peaks obtained. [36] Ovalbimin-sensitization from bronchoalveolar lavage fluid in mice…”

A new weapon for memory power: Elephantopus scaber (Linn.)

“…[17] The dose selection for memory activity was 150, 300, and 600 mg/kg of E. scaber leafy extract. Healthy male aged mice were divided into five groups with six mice in each.…”

“…Preliminary qualitative phytochemical screening of ethanolic extract of E. scaber showed the presence of flavonoids, phenols, steroids, tannins, terpenes, xanthoproteins, and sugar. [17] The biological actions of flavonoids within the nervous system are to enhance neuronal function, stimulate neuronal regeneration, control neuronal resistance to neurotoxins, including oxidants and inflammatory mediators and also responsible for neuronal differentiation, long-term potentiation, and memory. [18] It is postulated that anti-inflammatory and antioxidant drugs are useful in controlling the progression and inflammatory damage to brain tissue in Alzheimer's disease.…”