Evaluation of antibacterial activity of different mangrove plant extracts

Abstract

Antibacterial activity of mature leaves, tender leaves and bark extracts of Avicennia marina, Avicennia officinalis and Bruguiera sexangula were evaluated using Soxhelt extraction method. Petroleum ether, chloroform, ethyl acetate and ethanol were used as solvents in order to get the plant extracts. The antibacterial activity was screened by using agar diffusion technique against pathogenic bacteria species of Staphylococcus sp. (from urine), Proteus sp. (from a wound), Escherichia coli (from infected blood), Shigella sp. (from a wound) and Pseudomonas sp. (from a wound). The length of inhibition zone was measured in millimeters from the edge of the well to the edge of the inhibition zone. Twelve different plant extracts in A. marina, A. officinalis and B. sexangula exhibited different degree of growth inhibition against tested bacterial strains. Mature leaf extracts of A. marina and tender leaf extracts of A. officinalis in ethyl acetate exhibited promising antibacterial activity than other plant extracts. All plant extracts in ethyl acetate showed strong inhibition compared to other extracts on all tested bacterial strains. Among all bacterial strains, Pseudomonas sp. and E. coli showed considerable growth inhibition against almost all plant extracts. Mature leaf extract of only A. marina was used for further investigations since a large amount of tender leaves of A. officinalis was not readily available. Charcoal treated mature leaf extracts of A. marina showed more inhibition for all tested bacterial strains than untreated plant extracts. Combinations of mature leaf extracts of A. marina in different solvents failed to exhibit synergistic activity against tested bacterial strains. Most combinations showed antagonistic effect against Proteus sp., E. coli and Shigella sp. Antibacterial activity of plant extracts of A. marina gradually declined during 4 months after extraction, for all tested bacterial strains. Components of mature leaf extracts of A. marina in chloroform, ethyl acetate and ethanol were separated by Analytical Thin Layer Chromatography (ATLC) when the eluents were hexane and diethyl ether. Isolated components in Preparative Thin Layer Chromatography (PTLC) exhibited moderate antibacterial activity against Pseudomonas sp., Shigella sp. and E. coli. Separated components did not show any antibacterial activity against Proteus sp. and Staphylococcus sp. Phytochemical screening revealed that mature leaf of A. marina contained alkaloids, steroids, triterpenoids and flavonoids.

Screening of some plant materials used in South-West Algerian traditional medicine for their antibacterial activity

“…These bacterial strains may have some kind of resistance mechanisms e.g. enzymatic inactivation, target sites modification and decrease intracellular drug accumulation or the concentration of the compound used may not be sufficient [25]. Although, the low values recorded for some plant extracts may be attributed to the fact that the extracts being in crude form, contain very small amounts of bioactive compounds.…”

The Comparison Among Antibacterial Activity of Mespilus germanica Extracts and Number of Common Therapeutic Antibiotics “In Vitro”

“…These bacterial strains may have some kind of resistance mechanisms e.g. enzymatic inactivation, target sites modification and decrease intracellular drug accumulation [20] or the concentration of the compound used may not be sufficient. No inhibition was observed with controls, which proves that solvents could not act as antibacterial agents.…”

“…In the disk agar diffusion method 1.5×10 8 CFU/mL (equivalent to 0.5 McFarland standards) of standard culture of each strain was cultured on agar surface at the first step, and then it was spread on the surface of agar by sterile glass spreader. After the inoculated plates had dried sufficiently the discs were kept over the agar plates using sterile forceps at various concentrations (10,20,30, and 40 mg/mL) [14].…”

Induction of cytotoxicity by Bruguiera gymnorrhiza in human breast carcinoma (MCF-7) cell line via activation of the intrinsic pathway

“…[ 6 ] Various Mangrove species produce secondary metabolites with various bioactive properties such as insecticidal, antibacterial, antidiarrheal, cytotoxic, and antiviral. [ 7 8 9 10 11 12 13 14 15 16 ]…”