Evaluation of Antibacterial Activity of Selected Ethnomedicinal Plants for Poultry in Masaka District, Uganda

Nalubega, Rebecca; Kabasa, John David; Olila, D.; Kateregga, John

doi:10.3923/rjpharm.2011.18.21

Cited by 23 publications

(16 citation statements)

References 6 publications

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“…This is in line with earlier studies which attribute the observed differences to the variation in chemical composition and structure of cell wall of both types of microorganisms [23][24][25][26] .…”

Section: Antimicrobial Activitysupporting

confidence: 81%

Antioxidant and antimicrobial activities of selected medicinal plants from Algeria

2014

JCLM

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Section: Antimicrobial Activitysupporting

confidence: 81%

Antioxidant and antimicrobial activities of selected medicinal plants from Algeria

2014

JCLM

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“…After the medium got solidified, the plates were allowed to dry for one hour. The bacterial/yeast suspension equal 1.5×108 cells /ml in sterile normal saline was prepared following the method [20] and inoculated on nutrient agar media by sterile cotton swabs. Wells in 6 mm diameter were punctured in the media using sterile cork borers.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Antimicrobial Activity of <i>Opuntia cochenillifera</i> (L.) Mill Fruit and Cladode Extracts

Singh

Vidyasagar

2016

IJPPE

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Abstract. In the present study the antimicrobial activity of chloroform and methanolic extracts of Opuntia cochenillifera for both cladode and fruits was investigated. Methanolic extract was found to be an effective against the microbes namely, E. coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa Candida albican C.glabrata C.haemulonii C.Tropicalis. Maximum activity was observed against E. coli, B. Subtilis and, C.albican and C.glabrata at 40mg/ml. Agar well diffusion assay was used to determine minimum inhibitory concentration of all test microorganisms.

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“…Similar results were observed in the studies of , Manasa et al (2012), and Kekuda and Onkarappa (2015) which reported stronger inhibitory activity of extracts of Streptomyces species against Gram positive bacteria when compared to Gram negative bacteria. The low antibacterial activity of extracts of selected actinomycetes against the Gram negative bacteria could be ascribed to the presence of an outer membrane that possess hydrophilic polysaccharides chains and forms an additional barrier for extract as well as antibiotics (Lodhia et al, 2009;Nalubega et al, 2011). In earlier studies, Streptomyces species from Agumbe and Kodachadri (Shobha and Onkarappa, 2011) were shown to exhibit antifungal activity.…”

Section: Solvent Extracts Of Actinomycetes In Particularmentioning

confidence: 99%

Antimicrobial, Antioxidant and Cytotoxic Activity of <i>Streptomyces</i> species from Western Ghat Soils of Karnataka, India

Kekuda¹,

R²,

Gautham³

et al. 2016

Sci. Technol. Arts Res. J.

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Western Ghats of India represents one of the global biodiversity hotspots. The present study was carried out to isolate and determine antimicrobial, cytotoxic and antioxidant activity of actinomycetes from soils of different regions of Western Ghats of Shi Karnataka, India. A total of 182 isolates were recovered from 57 soil samples collected at 13 different regions of Western Ghat region of Shivamogga district by serial dilution technique. Out of 182 isolates, 17 isolates were chos determine antimicrobial, cytotoxic and antioxidant activity. Solvent extracts of these isolates exhibited varied antimicrobial potency against Gram positive and Gram negative bacteria, yeasts and molds. Ethyl acetate was found to be best solvent for extraction of bioactive metabolites from most of isolates followed by butanol and hexane. Cytotoxic activity, as evaluated by brine shrimp lethality bioassay, revealed the potency of extracts to cause dose dependent mortality of brine shrimp larvae. Solvent extracts exhibited dose dependent antioxidant activity, as evaluated by DPPH, ABTS, Metal chelating and Ferric reducing assays. Western Ghat actinomycetes appear to be promising resources of bioactive agents and can be exploited industrially. Further studies on recovery of active constituents from solvent extracts and their bioactivity determinations are to be carried out. Abstract Article InformationWestern Ghats of India represents one of the global biodiversity hotspots. The present study was carried out to isolate and determine antimicrobial, cytotoxic and antioxidant activity of actinomycetes from soils of different regions of Western Ghats of Shivamogga district, Karnataka, India. A total of 182 isolates were recovered from 57 soil samples collected at 13 different regions of Western Ghat region of Shivamogga district by serial dilution-plating technique. Out of 182 isolates, 17 isolates were chosen for solvent extraction and to determine antimicrobial, cytotoxic and antioxidant activity. Solvent extracts of these isolates exhibited varied antimicrobial potency against Gram positive and Gram negative bacteria, und to be best solvent for extraction of bioactive metabolites from most of isolates followed by butanol and hexane. Cytotoxic activity, as evaluated by brine shrimp lethality bioassay, revealed the potency of extracts to cause dose brine shrimp larvae. Solvent extracts exhibited dose dependent antioxidant activity, as evaluated by DPPH, ABTS, Metal chelating and Ferric reducing assays. Western Ghat actinomycetes appear to be promising resources of bioactive agents ed industrially. Further studies on recovery of active constituents from solvent extracts and their bioactivity determinations are to be carried out.

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Evaluation of Antibacterial Activity of Selected Ethnomedicinal Plants for Poultry in Masaka District, Uganda

Cited by 23 publications

References 6 publications

Antioxidant and antimicrobial activities of selected medicinal plants from Algeria

Antioxidant and antimicrobial activities of selected medicinal plants from Algeria

Antimicrobial Activity of <i>Opuntia cochenillifera</i> (L.) Mill Fruit and Cladode Extracts

Antimicrobial, Antioxidant and Cytotoxic Activity of <i>Streptomyces</i> species from Western Ghat Soils of Karnataka, India

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