Evaluation of antibacterial activity of crude protein extracts from seeds of six different medical plants against standard bacterial strains

Abstract

A huge group of natural antimicrobial compounds are active against a large spectrum of bacterial strains causing infectious threat. The present study was conducted to investigate the crude extracts of antimicrobial protein and peptide efficacy from six medicinal plant seeds. Extraction was carried out in Sodium phosphate citrate buffer, and Sodium acetate buffer using different pH. Antimicrobial activities of these plants were determined by the microbiological technique using Agar well diffusion Assay. Extremely strong activity was observed in the seed extracts of Allium ascolinicum extracted in sodium phosphate citrate buffer at pH (5.8) against Proteus vulgaris, Escherichia coli and Staphylococcus aureus with zone of inhibition 17 mm, 17 mm and 15 mm and Rumex vesicarius at pH (7.6), Ammi majus at pH (6.8), Cichorium intybus at pH (7.4) and Cucumis sativus at pH (7.8) also showed better sensitivity against the bacterial strains with zone of inhibition ranges 16-10 mm and some of the strains were found to be resistant. Antibacterial activity pattern of different plant extracts prepared in sodium acetate buffer pH (6.5), among all the plant seed extracts used Foeniculum vulgare had shown good inhibition in all the bacterial strains used, with zone of inhibition ranges 11-12.5 mm, The extracts of C. intybus and C. sativus were found to be effective with zone of inhibition 11-6 mm and some of the strains were found to be resistant. Most of the strains found to have shown better sensitivity compared with the standard antibiotic Chloramphenicol (25 mcg). Our results showed that the plants used for our study are the richest source for antimicrobial proteins and peptides and they may be used for industrial extraction and isolation of antimicrobial compounds which may find a place in medicine industry as constituents of antibiotics.

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“…Strong antimicrobial activity was observed in Allium ascolinicum seed extracts at pH (5.8) against Proteus vulgaris, Escherichia coli and Staphylococcus aureus with zone of inhibition 17 mm, 17 mm and 15 mm respectively. Antibacterial activity was also reported in seed extracts of Rumex vesicarius at pH (7.6), Ammi majus at pH (6.8), Cichorium intybus at pH (7.4), Cucumis sativus at pH (7.8) and Foeniculum vulgare at pH (6.5) 127 .…”

Polyphenolic and phytochemical content of Cucumis sativus seeds and study on mechanism of preservation of nutritional and quality outcomes in enriched mayonnaise

“…The pH of the control reached to 3.85 from the initial pH of 4.08 at week 1. The CSS exhibited the effective antimicrobial property (Akeel et al ., ), and the TPC and YMC were not affected more in the changes of pH and acid levels in enriched sample. The pH and acid values were observed at the minimum of 4.1 and 1.25 mg KOH g −1 for CSS‐enriched samples at 4 °C, respectively.…”

“…According to the World Health Organization (WHO, ), medicinal plants would be the best source to obtain a variety of bioactive compounds. Therefore, such plants should be investigated to better understand their properties, safety and efficiency (Akeel et al ., ). The incorporation of natural antioxidants, which are molecules capable of preventing or delaying the oxidative lipid damage represents a key alternative to overcome the quality deterioration of lipid‐based food products, provoked mainly by the attack of the reactive oxygen species (ROS) (Aleman et al ., ).…”

Antibacterial and Phytochemical Screening of Ethanol Extract of Hemidesmus indicus roots

“…For this reason many efforts have been made to discover new antimicrobial compounds from various kinds of sources such as microorganisms, animals, and plants. Fungi are also responsible for a number of diseases in humans and a number of fungal species work in association with some bacteria species; among them Candida albicans is responsible for a number of diseases [1][2][3] .…”