Evaluation of antibacterial and antioxidant potential of the zinc oxide nanoparticles synthesized by aqueous and polyol method

Soren, Siba; Kumar, Sanjeet; Mishra, S.; Jena, Padan Kumar; Verma, Shiv Kumar; Parhi, Purnendu

doi:10.1016/j.micpath.2018.03.048

Cited by 144 publications

(64 citation statements)

References 36 publications

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“…Recently, ZnO NPs have shown antimicrobial activity on skin-specific bacteria ( Aditya et al, 2018 ), Streptococcus mutans, Streptococcus pyogenes, Vibrio cholerae, Shigella flexneri , and Salmonella typhi ( Soren et al, 2018 ). It has also shown antimicrobial activity against methicillin resistant Staphylococcus aureus ( Kadiyala et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumannii

et al. 2018

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Acinetobacter baumannii is a multi-drug resistant opportunistic pathogen, which causes respiratory and urinary tract infections. Its prevalence increases gradually in the clinical setup. Carbapenems (beta-lactam) are most effective antibiotics till now against A. baumannii, but the development of resistance against it may lead to high mortality. Therefore, it is of utmost importance to develop an alternative drug against A. baumannii. In the present study, we have synthesized ZnO nanoparticle (ZnO-NP) and characterized by X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy and UV-Visible spectroscopy. Prepared ZnO-NPs have the size of 30 nm and have different characteristics of ZnO-NPs. Growth kinetics and disk diffusion assay showed that ZnO-NP demonstrated good antibacterial activity against carbapenem resistant A. baumannii. We have also investigated the mechanism of action of ZnO-NPs on the carbapenem resistant strain of A. baumannii. The proposed mechanism of action of ZnO involves the production of reactive oxygen species, which elevates membrane lipid peroxidation that causes membrane leakage of reducing sugars, DNA, proteins, and reduces cell viability. These results demonstrate that ZnO-NP could be developed as alternative therapeutics against A. baumannii.

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Section: Discussionmentioning

confidence: 99%

Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumannii

et al. 2018

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“…16-19 Provision of nano- and/or micro-sized particles helps ZnO promote the functional action of osteoblasts while increasing the bactericidal effects. 20 The construction of ZrO 2 nanoparticles (NPs) was found to improve the physicochemical properties of PC. 17,21…”

Section: Introductionmentioning

confidence: 99%

In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components

Rahimi

Salarinasab

Ghasemi

et al. 2019

J Dent Res Dent Clin Dent Prospects

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Background. The aim of this in vitro study was to investigate the effect of zinc oxide (ZnO) and zirconium oxide (ZrO2) microparticles (MPs) and nanoparticles (NPs) in combination with white Portland cement (WPC) on odontogenic capacity of human dental pulp stem cells over a period of 21 days. Methods. Synthesized ZnO and ZrO2 particles were characterized using scanning electron microscopy and transmission electron microscopy. The viability of human dental pulp stem cells was measured by a 3-(4,5-dimethylthiazolyl-2-yl)-2,5- diphenyltetrazolium bromide assay at 7-, 14- and 21-day intervals after seeding on WPC disks enriched with ZnO and ZrO2 MPs and NPs. Odontogenic potential of ZnO and ZrO2 particles in combination with WPC was investigated by alkaline phosphatase (ALP) activity and ionized calcium level of supernatant culture media at different time intervals. Data were analyzed using one-way ANOVA and post hoc Tukey tests. Results. All the materials exhibited cell viability over a 21-day period, except for WPC with ZnO NPs on day 7, although it was not statistically significant (P>0.05). The ALP activity and ionized calcium level increased in all the groups compared to the control group (P<0.05). ZnO NPs had superior effect on odontogenic activity and calcium ion release compared to ZnO MPs (P=0.046). There was no significant difference between ZrO2 MPs and NPs in odontogenic activity (P>0.05). Conclusion. WPC enriched with ZnO and ZrO2 increased ALP activity and calcium ion release of human dental pulp stem cells over a period of 21 days in vitro.

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“…The toxicity of ZnCl 2 to S. aureus is expected, with 2 mmol/L of Zn or much less reported to cause complete growth inhibition, depending on the strain of organism used,50 and zinc is generally biocidal to microbes in the 1–10 mmol/L range, depending on salinity and temperature 51. Zinc oxide nanoparticles have also been shown to effectively inhibit the growth of microbes such as E. coli and S. mutans 23,24,42. There are fewer reports of nZnO toxicity to S. aureus , although concentrations of around 100 mg/L of nZnO are reported to cause growth inhibition 52.…”

Section: Discussionmentioning

confidence: 99%

“…For example, nZnO was found to be an effective bactericide against Escherichia coli , as measured by Varaprasad et al23 In the latter study, the inhibition zone for the nano-zinc oxide containing fibers was between 2.1 and 3.6 mm in an agar diffusion plate test. A minimum of 100 µg/mL of nZnO in suspension was found to be antibacterial against both Gram-positive bacteria ( S. mutans and S. pyogenes ) and Gram-negative bacteria ( Vibrio cholerae, Shigella flexneri, and Salmonella typhii ) as measured by MIC assay after 12 hrs exposure in Muller–Hinton broth 24. The effect of particle shape and size on toxicity is still being debated 25.…”

Section: Introductionmentioning

confidence: 99%

<p>Multilayered composite coatings of titanium dioxide nanotubes decorated with zinc oxide and hydroxyapatite nanoparticles: controlled release of Zn and antimicrobial properties against <em>Staphylococcus aureus</em></p>

Gunputh¹,

Le²,

Besinis³

et al. 2019

IJN

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Purpose: This study aimed to decorate the surface of TiO 2 nanotubes (TiO 2 NTs) grown on medical grade Ti-6Al-4V alloy with an antimicrobial layer of nano zinc oxide particles (nZnO) and then determine if the antimicrobial properties were maintained with a final layer of nano-hydroxyapatite (HA) on the composite. Methods: The additions of nZnO were attempted at three different annealing temperatures: 350, 450 and 550 °C. Of these temperatures, 350°C provided the most uniform and nanoporous coating and was selected for antimicrobial testing. Results: The LIVE/DEAD assay showed that ZnCl 2 and nZnO alone were >90% biocidal to the attached bacteria, and nZnO as a coating on the nanotubes resulted in around 70% biocidal activity. The lactate production assay agreed with the LIVE/DEAD assay. The concentrations of lactate produced by the attached bacteria on the surface of nZnO-coated TiO 2 NTs and ZnO/HA-coated TiO 2 NTs were 0.13±0.03 mM and 0.37±0.1 mM, respectively, which was significantly lower than that produced by the bacteria on TiO 2 NTs alone, 1.09±0.30 mM (Kruskal–Wallis, P <0.05, n=6). These biochemical measurements were correlated with electron micrographs of cell morphology and cell coverage on the coatings. Conclusion: nZnO on TiO 2 NTs was a stable and antimicrobial coating, and most of the biocidal properties remained in the presence of nano-HA on the coating.

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Evaluation of antibacterial and antioxidant potential of the zinc oxide nanoparticles synthesized by aqueous and polyol method

Cited by 144 publications

References 36 publications

Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumannii

Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant Acinetobacter baumannii

In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components

<p>Multilayered composite coatings of titanium dioxide nanotubes decorated with zinc oxide and hydroxyapatite nanoparticles: controlled release of Zn and antimicrobial properties against <em>Staphylococcus aureus</em></p>

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