This study evaluates the potential of stingless bee (Tetragonula laeviceps) methanol extract and its fractions (hexane, dichloromethane, ethyl acetate, methanol, and acetone-water) as an antibacterial, antioxidant, phenolics content, and LC/MS profiles of dichloromethane fraction. The test was carried out by thinlayer chromatography (TLC) -bioautography (dot-blot and elution), then determined the MIC and IC 50 values and Antioxidant Activity Index (AAI). Antibacterial tests were carried out against Staphylococcus aureus and Escherichia coli, and antioxidant potential was carried out by the DPPH method. Total phenolic content was evaluated by the spectrophotometry method. The results showed that crude fraction, hexane, and dichloromethane fractions had moderate antibacterial activity against S. aureus (MIC values: 128-256 µg/mL) and weak antibacterial activity against E. coli. Fractions of dichloromethane, methanol, and acetone water had a very strong antioxidant activity with an AAI of 5.28, 2.17, 3.00, and 3.56, respectively. The LC/MS profiles showed that the dichloromethane fraction contained 5.7-dihydroxychromone, cnidimon C, puerarin, and irisflorentin. Fractions of dichloromethane, methanol, and acetone-water fraction are potential antioxidant and antibacterial sources.