Evaluation of antibacterial effects of different intracanal medicaments on Enterococcus faecalis in primary teeth: An in vitro study

Abstract

Objectives Successful endodontic therapy is based on the reduction of infecting bacteria by cleaning, shaping, and disinfecting of the root canal system, thus the use of intracanal dressing is necessary for optimal success of root canal treatment. This study was designed to evaluate the effect of chitosan and propolis as intracanal medicaments against Enterococcus faecalis compared to calcium hydroxide in primary root canals. Material and Methods Ninety‐six extracted primary second molars were collected. Teeth preparation was completed to size 30 K‐file. They were randomly divided into four groups; (A): chitosan, (B): propolis, (C): calcium hydroxide, and (D): control group (saline). The tooth specimens were inoculated with E. faecalis . Then, tested materials were applied for all groups in accordance to the groups each tooth belonged to. Following this, the bacterial colonies were counted after 24 h, 72 h, and 1 week of applying dressing materials and incubation. Finally, one–way analysis of variance and Fisher's least significant difference tests were used for statistical comparisons between the groups at a significance level of .05. Results No statistically significant difference was found between groups A, B, and C for both 24 h and a week ( p ≥ .05). Yet, a statistical difference between groups A, B, C, and D after 72 h and 1 week were seen ( p ≤ .05). Conclusions Chitosan and propolis medicaments were as effective as calcium hydroxide against E. faecalis in primary root canal treatment and might be considered as an alternative dressing material between treatment sessions.

Propolis efficiency against Enterococcus faecalis compared with other intra-canal medicaments: a systematic review with meta-analysis

“…In the most recent study, Shamma et al [27,31] also focused on primary teeth. The authors conducted in vitro research evaluating the effectiveness of different intracanal medicaments against E. faecalis bacteria in primary root canal systems.…”

Strategies and mechanisms targeting Enterococcus faecalis biofilms associated with endodontic infections: a comprehensive review

“…Propolis contains important pharmacologically active ingredients such as flavonoids, which can act on bacterial microbial membranes or cell walls and cause functional and structural damage ( Parolia et al., 2021 ). A comparative evaluation of the efficacy of propolis and Ca(OH) 2 as root canal drugs for Enterococcus faecalis showed that propolis has antibacterial activity comparable to Ca(OH) 2 ( Shamma et al., 2023 ).…”

Assessment of the Antimicrobial Effectiveness of Herbal Root Canal Irrigants (Propolis, Triphala, and Aloe Vera) and Chlorhexidine Against Enterococcus Faecalis

“…Propolis is an organic, biocompatible resinous material that is made from plant materials that honeybees collect. Propolis has antimicrobial and anti-inflammatory properties because it contains flavonoids [ 9 ].…”