Evaluation of antibacterial oxygen/ozone mixture in vitro activity on bacteria isolated from cervico-vaginal mucus of cows with acute metritis

Lillo, Edoardo; Cordisco, Marco; Trotta, Adriana; Greco, Grazia; Carbonari, Alice; Rizzo, Annalisa; Sciorsci, Raffaele Luigi; Corrente, Marialaura

doi:10.1016/j.theriogenology.2022.10.031

Cited by 8 publications

(5 citation statements)

References 34 publications

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“…Several beneficial effects of O 3 are widely described in the literature, i.e., antibacterial, antifungal, and antiviral properties [ 2 , 3 ]. The anti-inflammatory, analgesic, immunomodulatory, and healing properties have also been reported [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…Ozone (O 3 ), a molecule consisting of three oxygen atoms, has attracted the attention of medical practitioners for its powerful oxidizing properties, which can disrupt the structure of microorganisms and induce their inactivation [ 1 , 2 , 3 ]. The application of O 3 in medical settings is an existing research topic, although concerns regarding its safety and efficacy are still debated.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Antiviral and Virucidal Activity of Ozone against Feline Calicivirus

Catella,

Pellegrini,

Carbonari

et al. 2024

Animals

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The Caliciviridae family includes several viral pathogens of humans and animals, including norovirus (NoV), genus Norovirus, and feline calicivirus (FCV), genus Vesivirus. Due to their resistance in the environment, NoV and FCV may give rise to nosocomial infections, and indirect transmission plays a major role in their diffusion in susceptible populations. A pillar of the control of viruses resistant to an environment is the adoption of prophylaR1.6ctic measures, including disinfection. Since NoVs are not cultivatable in common cell cultures, FCV has been largely used as a surrogate of NoV for the assessment of effective disinfectants. Ozone (O3), a molecule with strong oxidizing properties, has shown strong microbicidal activity on bacteria, fungi, protozoa, and viruses. In this study, the virucidal and antiviral activities of an O3/O2 gas mixture containing O3 were tested at different concentrations (20, 35, and 50 μg/mL) for distinct contact times against FCV. The O3/O2 gas mixture showed virucidal and antiviral activities against FCV in a dose- and contact time-dependent fashion. Ozonation could be considered as a valid strategy for the disinfection of environments at risk of contamination by FCV and NoV.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro Antiviral and Virucidal Activity of Ozone against Feline Calicivirus

Catella,

Pellegrini,

Carbonari

et al. 2024

Animals

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“…There are several in vivo and in vitro studies published in the literature, addressing/demonstrating the therapeutic potential of O 3 in treating genital infections of farm animals [ 22 , 35 ] and documenting the disinfectant, immunomodulatory, and anti-inflammatory actions of O 3 . Inoculation of O 3 using foams into the vagina and uterus of cows affected by urovagina has been shown to decrease the “open days” and the number of artificial inseminations required to induce pregnancy in cows affected by urovagina.…”

Section: Discussionmentioning

confidence: 99%

“…An in-house method was developed to expose the Petri dishes to the O 3 gas flow, as previously described [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

“…O 3 is an allotropic form of oxygen, composed by three oxygen atoms, organized in a relatively unstable cyclic structure that makes it a powerful oxidant agent [ 20 ]. Due to this feature, it shows microbicidal and antimicrobial properties against bacteria, fungi, viruses, and protozoa [ 19 , 21 , 22 ]. As for the effects against viruses, O 3 causes structural damage by protein and lipid peroxidation of the envelope and capsid, respectively, and by the destruction of nucleic acids [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Activity of Ozone/Oxygen Gaseous Mixture against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis

Lillo

Pellegrini

Rizzo

et al. 2023

Animals

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Alphaherpesviruses cause genital lesions and reproductive failure in both humans and animals. Their control is mainly based on prevention using hygienic prophylactic measures due to the absence of vaccines and limitations of antiviral drug therapy. Ozone is an oxidating gas showing a strong microbicidal activity on bacteria, fungi, viruses, and protozoa. The present study assessed the in vitro virucidal and antiviral activity of ozone against caprine herpesvirus type 1 (CpHV-1). The virucidal activity of a gaseous mixture containing O3 at 20 and 50 μg/mL was assessed against the virus at different contact times (30 s, 60 s, 90 s, 120 s, 180 s, and 300 s). Antiviral activity of a gaseous mixture containing O3 at 20 and 50 μg/mL was evaluated against the virus after 30 s and 60 s. Ozone displayed significant virucidal activity when used at all the tested concentrations whilst significant antiviral activity was observed using ozone at 50 μg/mL. The gaseous mixture, tested in the present study, showed virucidal and antiviral activity against CpHV-1 in a dose- and time contact-dependent fashion. Ozone therapy could be evaluated in vivo for the treatment of CpHV-1-induced genital lesions in goats using topical applications.

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