Evaluation of Antibacterial Property of Cell-free Hemolymph and Hemocytes of Marine Gastropod, Rapana Rapiformis from Inshore Waters of Pondicherry, Southeast Coast of India

Amruthalakshmi, S. A.; Yogamoorthi, A.

doi:10.18034/mjmbr.v4i1.423

Cited by 1 publication

(3 citation statements)

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“…Molluscs, which belong to the invertebrates, are a potentially rich source of bioactive molecules with antimicrobial properties, which has been proven in numerous studies [ 9 , 10 , 11 , 12 ]. The evolutionary survival of these organisms indicates that their immune system defenses are extremely efficient [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, there has been a growing interest in marine natural Pharmaceuticals 2024, 17, 68 2 of 19 products or marine-borne secondary metabolites [8]. However, studies on the antibacterial properties of the hemolymph of gastropods are very meager scarce [9].…”

Section: Introductionmentioning

confidence: 99%

“…Some studies have focused on the entirety of the body fluid, showing its antibacterial features [19,20], and others show data on the differences in the hemolymph components regarding its antibacterial activity. A study by Amruthalakshmi and Yogamoorthi (2017) demonstrates the differences in hemocytes and hemocyte-free hemolymph against different pathogenic bacteria (Klebsiella pneumonia, Staphylococcus aureus, Pseudomonas aeruginosa, Vibrio cholera, and Escherichia coli) [9]. Tetreau et al identified highly active proteins in hemocyte-free hemolymph from Biomphalaria glabrata [21].…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial Action of Protein Fraction Isolated from Rapana venosa Hemolymph against Escherichia coli NBIMCC 8785

Kirilova,

Topalova,

Velkova

et al. 2024

Pharmaceuticals

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Natural products and especially those from marine organisms are being intensively explored as an alternative to synthetic antibiotics. However, the exact mechanisms of their action are not yet well understood. The molecular masses of components in the hemolymph fraction with MW 50–100 kDa from Rapana venosa were determined using ImageQuant™ TL v8.2.0 software based on electrophoretic analysis. Mainly, three types of compounds with antibacterial potential were identified, namely proteins with MW at 50.230 kDa, 62.100 kDa and 93.088 kDa that were homologous to peroxidase-like protein, aplicyanin A and L-amino acid oxidase and functional units with MW 50 kDa from R. venous hemocyanin. Data for their antibacterial effect on Escherichia coli NBIMCC 8785 were obtained by CTC/DAPI-based fluorescent analysis (analysis based on the use of a functional fluorescence probe). The fluorescent analyses demonstrated that a 50% concentration of the fraction with MW 50–100 kDa was able to eliminate 99% of the live bacteria. The antimicrobial effect was detectable even at a 1% concentration of the active compounds. The bacteria in this case had reduced metabolic activity and a 24% decreased size. The fraction had superior action compared with another mollusc product—snail slime—which killed 60% of the E. coli NBIMCC 8785 cells at a 50% concentration and had no effect at a 1% concentration. The obtained results demonstrate the high potential of the fraction with MW 50–100 kDa from R. venosa to eliminate and suppress the development of Escherichia coli NBIMCC 8785 bacteria and could be applied as an appropriate component of therapeutics with the potential to replace antibiotics to avoid the development of antibiotic resistance.

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