Evaluation of antibiotic resistance dissemination by wastewater treatment plant effluents with different catchment areas in Germany

Alexander, Johannes; Hembach, Norman; Schwartz, Thomas

doi:10.1038/s41598-020-65635-4

Cited by 113 publications

(78 citation statements)

References 34 publications

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“…After molecular fingerprinting using partial dna J gene fragments, the topology of the neighbor-joining method (NJ) tree pointed out seven clusters which overlapped the seven phylogroups of Klebsiella with high bootstrap values ( Figure 1 ). The 52 sequences derived from this study fell into the cluster of Klebsiella pneumoniae sensu stricto (Kp1), a common inhabitant of wastewater [ 12 , 19 ]. Discussions regarding carbapenemase-producing Klebsiella pneumoniae from clinical settings have dominated research in recent years, but the presence of this important pathogen in the environment has rarely been studied.…”

Section: Resultsmentioning

confidence: 99%

“…The dissemination of antibiotic resistance genes (ARGs) worldwide, especially the carbapenemase family (CRG), became an urgent global health threat [ 9 , 10 ], mostly because their presence was detected in both clinical and environmental settings [ 11 ]. Wastewaters represent a hotspot for the development and dissemination of AMR and ARGs [ 12 ], being a key factor in this process [ 13 ]. Clinical isolates reaching wastewaters contribute to spreading ARGs to non-pathogenic bacteria, leading to new generations of ARBs with different and more aggressive resistance patterns [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Wastewaters, with or without Hospital Contribution, Harbour MDR, Carbapenemase-Producing, but Not Hypervirulent Klebsiella pneumoniae

et al. 2021

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Carbapenemase-producing Klebsiella pneumoniae (CPKP) isolated from influent (I) and effluent (E) of two wastewater treatment plants, with (S1) or without (S2) hospital contribution, were investigated. The strains belonged to the Kp1 phylogroup, their highest frequency being observed in S1, followed by S2. The phenotypic and genotypic hypervirulence tests were negative for all the strains tested. At least one carbapenemase gene (CRG), belonging to the blaKPC, blaOXA-48, blaNDM and blaVIM families, was observed in 63% of CPKP, and more than half co-harboured two to four CRGs, in different combinations. Only five CRG variants were observed, regardless of wastewater type: blaKPC-2, blaNDM-1, blaNDM-6, blaVIM-2, and blaOXA-48. Sequence types ST258, ST101 and ST744 were common for both S1 and S2, while ST147, ST525 and ST2502 were found only in S1 and ST418 only in S2. The strains tested were multi-drug resistant (MDR), all being resistant to beta-lactams, cephalosporins, carbapenems, monobactams and fluoroquinolones, followed by various resistance profiles to aminoglycosides, trimethoprim-sulphamethoxazole, tigecycline, chloramphenicol and tetracycline. After principal component analysis, the isolates in S1 and S2 groups did not cluster independently, confirming that the antibiotic susceptibility patterns and gene-type profiles were both similar in the K. pneumoniae investigated, regardless of hospital contribution to the wastewater type.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Wastewaters, with or without Hospital Contribution, Harbour MDR, Carbapenemase-Producing, but Not Hypervirulent Klebsiella pneumoniae

et al. 2021

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“…It has been confirmed that wastewater treatment plant effluents are influenced by hospital wastewaters [ 114 ] in Germany. Alexander et al [ 114 ] quantified the abundances of antibiotic resistance genes and facultative pathogenic bacteria as well as one mobile genetic element in genomic DNA via qPCR from 23 different wastewater treatment plant effluents in Germany. Total of 12 clinically relevant antibiotic resistance genes were categorized into frequently, intermediately, and rarely occurring genetic parameters of communal wastewaters.…”

Section: Usage Of Antibiotics In Different Countries Of Eu Region mentioning

confidence: 99%

Antimicrobial Resistance in Escherichia coli Strains Isolated from Humans and Pet Animals

Puvača

Frutos

2021

Antibiotics

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Throughout scientific literature, we can find evidence that antimicrobial resistance has become a big problem in the recent years on a global scale. Public healthcare systems all over the world are faced with a great challenge in this respect. Obviously, there are many bacteria that can cause infections in humans and animals alike, but somehow it seems that the greatest threat nowadays comes from the Enterobacteriaceae members, especially Escherichia coli. Namely, we are witnesses to the fact that the systems that these bacteria developed to fight off antibiotics are the strongest and most diverse in Enterobacteriaceae. Our great advantage is in understanding the systems that bacteria developed to fight off antibiotics, so these can help us understand the connection between these microorganisms and the occurrence of antibiotic-resistance both in humans and their pets. Furthermore, unfavorable conditions related to the ease of E. coli transmission via the fecal–oral route among humans, environmental sources, and animals only add to the problem. For all the above stated reasons, it is evident that the epidemiology of E. coli strains and resistance mechanisms they have developed over time are extremely significant topics and all scientific findings in this area will be of vital importance in the fight against infections caused by these bacteria.

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“…The presence of multi-resistance bacteria in foods is of concern to public health. Recently, evaluation of antibiotic resistance dissemination by wastewater treatment plant (WWTP) effluents with different catchment areas in Germany revealed that the daily discharge of K. pneumoniae in food-producing impacted WWTP effluents is higher compared to communal and hospital-impacted WWTP effluents [ 55 ].…”

Section: Spatio-temporal Distribution Of K Pneumoniae mentioning

confidence: 99%

The Animal-foods-environment interface of Klebsiella pneumoniae in Germany: an observational study on pathogenicity, resistance development and the current situation

Wareth

Neubauer

2021

Vet Res

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Klebsiella (K.) pneumoniae as a multi-drug resistant (MDR) pathogen is an emerging challenge for clinicians worldwide. Virulence factors are capsular antigens, adherence factors, the O-lipopolysaccharide, and siderophores promoting infectivity. Mechanisms of antimicrobial resistance are inactivation of compounds via enzymes, change of membrane permeability, and alteration of the target site of the antimicrobial compound. In addition to environmental resistance, K. pneumoniae can survive increasing concentrations of disinfectants, if exposed. This review describes the temporal and spatial distribution of K. pneumoniae in the past decades in Germany, with emphases on the development of resistance in the non-human columns of the One-Health concept. In general, K. pneumoniae is a neglected pathogen in veterinary and environmental health, and the risk of human infection concerning animal contact and food consumption is barely investigated. Few reports exist (n = 26) on antibiotic resistance of isolates from non-human origin. Multi-drug resistance and extended-spectrum β-lactamase (MDR-ESBL) strains also resistant to carbapenems and antibiotics of the ß-lactam group harbor blaCTX-M, blaOXA, blaTEM, blaSHV, blaCMY, and PMQR have been found in animals, foods, and the environment. Colistin resistant strains carrying the mcr-1 gene were detected in wastewater. The blaCTX-M-15 and blaOXA-48 genes are the most frequently identified AMR genes in isolates of humans and were also the most predominant ESBL-genes in samples collected from animal hosts. Several aspects of the molecular epidemiology and resistance development of K. pneumoniae in farm animal populations, wildlife, and foods need intensive research. Environmental health has to be integrated into national research plans, as a lack of data is apparent. Increasing awareness of the fact that non-human sources can act as a reservoir for this pathogen has to be raised.

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Evaluation of antibiotic resistance dissemination by wastewater treatment plant effluents with different catchment areas in Germany

Cited by 113 publications

References 34 publications

Wastewaters, with or without Hospital Contribution, Harbour MDR, Carbapenemase-Producing, but Not Hypervirulent Klebsiella pneumoniae

Wastewaters, with or without Hospital Contribution, Harbour MDR, Carbapenemase-Producing, but Not Hypervirulent Klebsiella pneumoniae

Antimicrobial Resistance in Escherichia coli Strains Isolated from Humans and Pet Animals

The Animal-foods-environment interface of Klebsiella pneumoniae in Germany: an observational study on pathogenicity, resistance development and the current situation

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