Evaluation of antibody response and nonspecific lymphocyte blastogenesis following inoculation of a live attenuated bluetongue virus vaccine in goats

Lacetera, Nicola; Ronchi, Bruno

doi:10.2460/ajvr.2004.65.1331

Cited by 12 publications

(3 citation statements)

References 19 publications

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“…Documented side effects in sheep included severe to mild clinical signs [78], decreased milk production [76], transplacental infection [29-31], a transient reduction of semen quality in rams [79] and the suppression of non-specific lymphocyte blastogenesis when used in goats [80]. There is also experimental evidence that MLVs may have an increased potential for secretion in the semen of older bulls as well as rams [28].…”

Section: Introductionmentioning

confidence: 99%

Bluetongue: a historical and epidemiological perspective with the emphasis on South Africa

Coetzee

Stokstad

Venter

et al. 2012

Virol J

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Bluetongue (BT) is a non-contagious, infectious, arthropod transmitted viral disease of domestic and wild ruminants that is caused by the bluetongue virus (BTV), the prototype member of the Orbivirus genus in the family Reoviridae. Bluetongue was first described in South Africa, where it has probably been endemic in wild ruminants since antiquity. Since its discovery BT has had a major impact on sheep breeders in the country and has therefore been a key focus of research at the Onderstepoort Veterinary Research Institute in Pretoria, South Africa. Several key discoveries were made at this Institute, including the demonstration that the aetiological agent of BT was a dsRNA virus that is transmitted by Culicoides midges and that multiple BTV serotypes circulate in nature. It is currently recognized that BT is endemic throughout most of South Africa and 22 of the 26 known serotypes have been detected in the region. Multiple serotypes circulate each vector season with the occurrence of different serotypes depending largely on herd-immunity. Indigenous sheep breeds, cattle and wild ruminants are frequently infected but rarely demonstrate clinical signs, whereas improved European sheep breeds are most susceptible. The immunization of susceptible sheep remains the most effective and practical control measure against BT. In order to protect sheep against multiple circulating serotypes, three pentavalent attenuated vaccines have been developed. Despite the proven efficacy of these vaccines in protecting sheep against the disease, several disadvantages are associated with their use in the field.

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Section: Introductionmentioning

confidence: 99%

Bluetongue: a historical and epidemiological perspective with the emphasis on South Africa

Coetzee

Stokstad

Venter

et al. 2012

Virol J

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“…The blastogenesis of mononuclear cells can be monitored by the in vitro blast transformation test (Lacetera and Ronchi 2004). This technique allows the final calculation of the blast index, a parameter that indicates the stimulation or inhibition of cell growth.…”

Section: Resultsmentioning

confidence: 99%

Taraxacum Officinale Alcoholic Extract Augments the Cell-Mediated Immune Response in Anti-Anthrax Vaccinated Sheep

Pojar¹,

Şandru²,

Pastor³

et al. 2021

BUASVMCN-VM

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Phytotherapy used various preparations in both human and veterinary medicine over time, due to their increased bioavailability and lower costs. Taraxacum officinale of Compositae family, well-known for its therapeutic effects, abundantly grows on Romanian pastures. The research aimed to investigate its alcoholic extract’s effects on cell-mediated immunity in anti-anthrax vaccinated, grazing sheep. Local Turcana ewes kept on pasture from spring to fall were sc vaccinated with R1190 attenuated anthrax strain, and blood was sampled before and 14 days after the vaccination. In vitro cell-mediated immunity was monitored employing phagocytosis and blast transformation tests in the presence of T. officinale alcoholic extract. The extract increased phagocytosis two weeks after the vaccination (p<0.001), while the blastogenic response also showed significant variations (p<0.05) over time. There was a positive effect of T. officinale extract on the cellular immunity, suggesting its stress alleviating effects in anti-anthrax vaccinated sheep.

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“…The clinical reaction index (CRI) developed for the evaluation of BTV vaccine was used to quantify the observed clinical reactions post-challenge of vaccinated and post-inoculation of control sheep [5,6]. In short the CRI score (0-5) was calculated as the sum of the following: temperature score, i.e.…”

Section: Methodsmentioning

confidence: 99%

Attenuated Live Bluetongue Virus 8 Vaccine Protects Sheep from Challenge with the European BTV-8

Dungu¹,

Louw²,

Potgieter³

et al. 2008

TOVSJ

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An attenuated live bluetongue virus (AL-BTV) serotype 8 vaccine of South African origin was evaluated for its ability to protect sheep against challenge with a European BTV-8 isolated from an outbreak. Two groups of sero-negative sheep were vaccinated with either a single or booster vaccination and challenged subcutaneously 28 days following vaccination.Groups of vaccinated challenged sheep showed no clinical signs typical to Bluetongue disease, as compared to unvaccinated inoculated sheep of a control group. A clinical reaction index (CRI) of values ranging from 0 to 5 was determined for each of the groups, where vaccinates measured a CRI of less than 1 and the control sheep measured a CRI of at least 4.5.All vaccinated sheep developed high serotype-specific neutralizing antibodies post-vaccination. The results indicated that a single dose of the AL-BTV-8 was sufficient to protect vaccinated sheep against a virulent challenge of the European BTV-8 outbreak isolate.

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Evaluation of antibody response and nonspecific lymphocyte blastogenesis following inoculation of a live attenuated bluetongue virus vaccine in goats

Abstract: Inoculation of goats with the monovalent live attenuated BTV serotype 2 vaccine described herein resulted in a profound depression of nonspecific lymphocyte blastogenesis, which might compromise the resistance of vaccinated goats to pathogens.

Cited by 12 publications

References 19 publications

Bluetongue: a historical and epidemiological perspective with the emphasis on South Africa

Bluetongue: a historical and epidemiological perspective with the emphasis on South Africa

Taraxacum Officinale Alcoholic Extract Augments the Cell-Mediated Immune Response in Anti-Anthrax Vaccinated Sheep

Attenuated Live Bluetongue Virus 8 Vaccine Protects Sheep from Challenge with the European BTV-8

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