Evaluation of anticonvulsant, antimicrobial and hemolytic activity of <i>Aitchisonia rosea</i>

Abstract

&lt;p class="Abstract"&gt;The purpose of this study was to evaluate the anticonvulsant, antimicrobial and hemolytic effect of &lt;em&gt;Aitchisonia rosea&lt;/em&gt;. The anticonvulsant effect was studied at doses 400 and 800 mg/kg against pentylenetetrazole, strychnine and picrotoxin-induced seizures in albino mice. The antimicrobial assay was conducted by disc diffusion method and minimum inhibitory concentration. Hemolytic effect was analyzed by reported method. Phenolic compounds present in the n-butanol fraction of the plant were estimated by HPLC. The plant showed maximum response against drug-induced convulsions and provided protection to animals at both doses. It also showed maximum zone of inhibition and highly significant MIC against all bacterial and fungal strains. The plant protected the RBCs from hemolysis. The highest amount of phenolics found was caffeic acid (7.5 ± 0.04).&lt;/p&gt;&lt;p&gt; &lt;/p&gt;

Evaluation of Anti-Inflammatory and Analgesic Activities of Methanolic Extract of Aitchisonia Rosea in Albino Mice

“…ARME significantly and dose dependently decreased the paw licking time in second phase that might be owing to the inhibition of inflammatory mediators, so it acts more like NSAIDs in reducing pain. Accordingly, current findings have avowed that crude extract of Aitchisonia rosea possess preeminent potential to alleviate inflammation accompanied by algesia and may be allied to the presence of various phytoconstituents, as have been earlier reported in Aitchisonia rosea i.e., alkaloids, terpenoids, flavonoids, tannins and phenolics (quercetin, caffeic acid, chlorogenic acid and syringic acid) [4]. In addition, free radical scavenging and reducing potential of Aitchisonia rosea has previously been measured by using DPPH and reducing power assay [5].…”

“…It was then filtered using Whattman filter paper. The Aitchisonia rosea methanol extract (ARME) was concentrated and kept at 4 °C in a refrigerator [4].…”

“…Aitchisonia rosea Hemsl. ex Aitch (Family: Rubiaceae) plant is a native to Pakistan (Baluchistan), Afghanistan and Iran [4]. Traditionally it is used for the treatment of dermal infections in Baluchistan.…”

“…The aerial parts of Aitchisonia rosea have been reported to possess anti-convulsant, antimicrobial and hemolytic effect [4]. Indeed, it has been confirmed that Aitchisonia rosea extract exhibit robust anti-oxidant potential against reducing power assay and DPPH free radical scavenging assay [5], mainly attributed to its polyphenolic phytoconstituents, identified through HPLC technique [4]. A large number of flavonoids and other phenolics have been proved their noteworthy effects on inflammatory processes [6].…”

Effect of Artemisia persica on seizure severity and memory and learning disorders in pentylenetetrazole-kindled mice

“…Hence, it will be essential to pay attention to traditional medicine and herbal medicines to achieve low-risk drugs with minimal side effects (Rostampour et al, 2014). The plants such as Aitchisonia rosea (Rasool et al, 2015), Bacopa monnieri (Paulose et al, 2008), Cannabis sativa (Leite and Carlini, 1973), Cyperus articulates (Bakanova and Leutskií, 2971), Sechium edule (Mumtaz et al, 2012), Silybum marianum (Waqar et al, 2016) and Zizyphus jujube (Puhaja et al, 2011) showed antiepileptic effect. (Wan et al, 2017), and sedative (Amos et al, 2003) effects in animal models.…”