Evaluation of antidiarrhoeal activity of methanol extract of Combretum hypopilinum Diels (Combretaceae) leaves in mice

Ahmad, Mubarak Hussaini; Zezi, Abdulkadir Umar; Anafi, Sherifat Bola; Mohammed, Mustapha; Danraka, Rabi’u Nuhu; Alhassan, Zakariyya

doi:10.31024/apj.2020.5.2.3

Cited by 9 publications

(4 citation statements)

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“…Therefore, the non-toxic effects and lack of death by the ASE after the acute administration indicates that its LD 50 may be more than 5,000 mg/kg. The findings concur with a previous report on Piper capense (Wamba et al, 2020), Combretum hypopilinum (Ahmad et al, 2020) and Stachytarpheta cayennensis (Olayode et al, 2020).…”

Section: Discussionsupporting

confidence: 92%

Toxicological study on methanol root bark extract ofAcacia sieberiana(Fabaceae) in Wistar rats

Watafua

Ejiofor

Musa

et al. 2022

Preprint

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Background: The plant Acacia sieberiana belongs to the family Fabaceae. It has been used in ethnomedical practice to manage bleeding, rheumatism, pain, pyrexia, kidney diseases, gastrointestinal problems, parasitic and infectious diseases, hepatitis, cough, epilepsy, mouth ulcer and many more. Phytochemical compounds such as ellagic acid, quercetin, isoferulic acid, gallic acid, kaempferol, luteolin, apigenin, glucoside dihydroacacipetalin, acacipetalin and many others were isolated from Acacia sieberiana. Previous pharmacological investigations have reported that the plant has anticancer, antimicrobial, antidiarrhoeal and antitrypanosomal effects. Despite the therapeutic properties of this plant, no safety information is available in the literature. Hence, this work intends to investigate the sub-acute toxicity effects of Acacia sieberiana root bark extract (ASE). The phytochemical and oral median lethal dose (LD50) evaluations on the ASE were done in line with the standard protocols. The sub-acute toxic effects of the ASE (250, 750, and 1,500 mg/kg) were investigated following administration of the ASE daily for 28-consecutive days based on the Organization of Economic Cooperation and Development (OECD) 407 protocols in rats. The weekly body weights were monitored and the rats were euthanized on the 29th day. The blood samples from the animals were obtained for biochemical and haematological determinations. The liver, kidney, lung and heart were removed for histological investigations.

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Section: Discussionsupporting

confidence: 92%

Toxicological study on methanol root bark extract ofAcacia sieberiana(Fabaceae) in Wistar rats

Watafua

Ejiofor

Musa

et al. 2022

Preprint

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“…Therefore, the findings in the current study have shown that the LD 50 of the MECA orally could be more than 5000 mg/kg. Previous research reported by Ahmad et al (2020aAhmad et al ( , 2020b revealed that the oral LD 50 of Combretum hypopilinum was higher than 5000 mg/kg. In another study by Yerima et al (2014), the oral LD 50 of the Tamarindus indica bark was also greater than 5000 mg/kg.…”

Section: Discussionmentioning

confidence: 89%

Potential pharmacological properties of methanol leaves extract of Culcasia Angolensis (Araceae): antinociceptive, antiinflammatory and antipyretic activities in laboratory animals

Nock

Abraham

Ahmad

2022

JoBAZ

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Background Pain, inflammation and fever are serious conditions that are associated with various disease conditions. In modern medicine, non-steroidal anti-inflammatory drugs (NSAIDs), opioids together with corticosteroids have been considered to manage algesia and inflammation-related conditions. However, these conventional drugs are not affordable, not readily available, particularly to people living in rural areas in developing nations. Besides, they are associated with undesirable pharmacological actions. Generally, medicinal plants have been employed to manage various ailments. In Northern-Nigeria, the leaves of Culcasia angolensis (Araceae) are used traditionally to manage pain, fever and inflammation. However, scientific data validating its folkloric claim in treating pain and inflammatory-related abnormalities are not available. Hence, the current study aims to validate the antinociceptive, anti-inflammatory and antipyretic potentials of the methanol leaf extract of Culcasia angolensis (MECA). Phytochemical and acute toxicity effects of the MECA were conducted as per standard experimental procedures. The analgesic potential of the MECA was determined using abdominal writhing elicited by acetic acid and hot plate tests in mice. The actions of the MECA on acute inflammation were conducted using formalin-induced hind paw oedema and carrageenan-induced paw oedema. The Brewer's yeast-induced pyrexia was employed to check its antipyretic effect. Results The MECA inhibited abdominal writhes produced by acetic acid administration (p < 0.05) and elevated the pain threshold in the hot plate test. The MECA also reduced the formalin-induced paw oedema. Besides, it produced an effective (p < 0.05) and dose-dependent action against oedema produced by carrageenan and reduced the rectal temperature against the pyrexia caused by Brewer's yeast administration. Conclusions The outcome of the study suggests that the MECA could possess pharmacologically active constituents with antinociceptive, anti-inflammatory and antipyretic properties. Therefore, the results justified its ethnomedicinal use to manage pain and inflammatory-associated conditions.

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“…Phytochemical analysis plays a pivotal role in unraveling the intricate chemical composition of plant extracts, shedding light on bioactive compounds that may contribute to their pharmacological effects. Simultaneously, the investigation seeks to ascertain the acute toxicity profile through LD50 determination [1], ensuring a thorough evaluation of the extract's safety. In accordance with internationally recognized protocols, this study will utilize mice models to establish the dosage at which the Cassia sieberiana leaf extract becomes lethal to 50% of the tested population.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Investigation and Toxicological Insights of Cassia sieberiana Leaf Extract: Implications for Medicinal use

Enenche,

Uzoeto,

Difa

et al. 2024

JAMPS

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Introduction: Cassia sieberiana, a member of the Fabaceae family, has a rich history of traditional medicinal uses. This study focuses on exploring the medicinal potential of the methanol extract from Cassia sieberiana leaves. The research aims to conduct a comprehensive analysis of bioactive compounds and assess acute toxicity through LD50 determination. Methods: Fresh leaves were collected from Opi town, Nsukka, Nigeria, and authenticated. Male Wister albino rats were acclimatized, and phytochemical screening was performed using qualitative and quantitative methods. LD50 determination followed internationally recognized protocols, employing mice models. Results: The methanol extract exhibited a yield of 11.2%. Phytochemical analysis revealed the presence of proteins, carbohydrates, tannins, alkaloids, steroids, cardiac glycosides, saponins, flavonoids, reducing sugars, terpenoids, and quinones. Alkaloids were predominant (1770.8±74.43 mg/100g). LD50 determination showed 100% survival at 5000 mg/kg, indicating relative safety. Discussion: The high yield suggests methanol as an effective solvent. Phytochemical composition aligns with traditional uses, and the prevalence of alkaloids supports reported medicinal applications. Low phenolic content suggests antioxidant effects, while flavonoids may contribute to anti-inflammatory properties. Saponins and tannins indicate potential for antimicrobial and purgative use. The study affirms the safety of the methanol leaf extract. Conclusion: The study provides valuable insights into the medicinal potential of Cassia sieberiana. The significant alkaloid content, diverse bioactive compounds and demonstrated safety in LD50 determination support its traditional uses. These findings lay a robust foundation for further exploration of Cassia sieberiana in drug development and healthcare applications.

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Evaluation of antidiarrhoeal activity of methanol extract of Combretum hypopilinum Diels (Combretaceae) leaves in mice

Cited by 9 publications

References 0 publications

Toxicological study on methanol root bark extract ofAcacia sieberiana(Fabaceae) in Wistar rats

Toxicological study on methanol root bark extract ofAcacia sieberiana(Fabaceae) in Wistar rats

Potential pharmacological properties of methanol leaves extract of Culcasia Angolensis (Araceae): antinociceptive, antiinflammatory and antipyretic activities in laboratory animals

Phytochemical Investigation and Toxicological Insights of Cassia sieberiana Leaf Extract: Implications for Medicinal use

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