Evaluation of antifungal activity in essential oil of the Syzygium aromaticum (L.) by extraction, purification and analysis of its main component eugenol

Abstract

Antifungal properties of some essential oils have been well documented. Clove oil is reported to have strong antifungal activity against many fungal species. In this study we have evaluated antifungal potential of essential oil of Syzygium aromaticum (L.) against some common fungal pathogens of plants and animals namely, Fusarium moniliforme NCIM 1100, Fusarium oxysporum MTCC 284, Aspergillus sp., Mucor sp., Trichophyton rubrum and Microsporum gypseum. All fungal species were found to be inhibited by the oil when tested through agar well diffusion method. Minimum inhibitory concentration (MIC) was determined for all the species. Column chromatography was performed to separate the eugenol rich fraction from clove oil. Out of seven fractions maximum activity was obtained in column fraction II. TLC and HPLC data confirmed presence of considerable Eugenol in fraction II and clove oil. Microscopic study on effect of clove oil and column fraction II on spores of Mucor sp. and M. gypseum showed distortion and shrinkage while it was absent in other column fractions. So it can be concluded that the antifungal action of clove oil is due to its high eugenol content.

Eugenol oil nanoemulsion: antifungal activity against Fusarium oxysporum f. sp. vasinfectum and phytotoxicity on cottonseeds

“…TLC analysis showed that Eugenol was present as a main component in the three EON tested concentration, as the colored zone showed the same R f value comparable with eugenol standard. Similar fractionation was performed using TLC analysis to identify the active antifungal components in clove oil (Park et al 2007;Rana et al 2011).…”

Candidiasis: Predisposing Factors, Prevention, Diagnosis and Alternative Treatment

“…It is very important to underline that the use of plants and other botanical preparations are used, since ancient times, by primitive societies, due to healing properties and therapeutic and psychotherapeutic benefits. It is important to address the fact that plants are widely used to obtain a large variety of drugs, being used in folk medicine for a long time [26][27][28][29][32][33][34][35]. Concerning that plants represent an economic, easily and which are accessible for all of people, they can be used into a variety of diseases, constituting an excellent source of biomolecules able to act like natural antifungal agents and, at the same time, to encourage the search for other alternative treatments.…”

Activity of phenolic compounds from plant origin against Candida species

“…More and more, people worldwide use medicinal plants as first priority to maintain a good health, to prevent or even treat some health conditions. Linked to this, some studies have been conducted, using a wide variety of plants, towards the identification of bioactive molecules, their properties and mechanisms of action (Agarwal et al, 2010;Alves-Silva et al, 2013;Asgarpanah and Kazemivash, 2012;Asl and Hosseinzadeh, 2008;Bakkali et al, 2008;Kanafani and Perfect, 2008;Rana et al, 2011;Sher, 2009;Shojaii and Fard, 2012;Silva et al, 2011b;Singh et al, 2010). In fact, natural matrices, in particular, plants, are extremely rich sources of biomolecules that have sparked an increasing interest for scientific researchers, in different areas of knowledge.…”