Evaluation of Antifungal Potential of Allium Ampeloprasum Against Fungal Phytopathogens

Abstract

Objective: Present study evaluates the antifungal potential of Allium ampeloprasum extracts against two phytopathogens of economic importance, namely Alternaria triticina and Magnaporthe oryzae which are found to be one of the greatest threats to wheat and rice crop worldwide.Methods: Four different organic solvents, i.e., water, methanol, acetone, and hexane were used for extract preparation based on the increasing order of polarity. Further, different concentrations of these extracts were used to perform a quantitative assay for measuring the antifungal activity of extracts. Two-way analysis of variance was used to statistically analyze the results through GraphPad Prism v. 7.00.Results: The best results were shown by water extracts with a maximum inhibition of 99.39±0.2% against A. triticina and 99.39±2.2% against M. oryzae. Water extracts gave minimal inhibitory concentration 90 values at 9.94 μg/mL against M. oryzae and at 16.73 μg/mL against A. triticina.Conclusion: Water extracts of A. ampeloprasum have relatively higher potential for being used as an antifungal agent as compared to other organic extracts used in this study. Further study on the chemical composition of A. ampeloprasum water extracts can reveal potent anti-phytopathogenic fungal compounds which can be used to develop biofungicides.

Inventory and Management of Fungi Associated with Banana Plant through the Use of <i>Allium ampeloprasum</i> and <i>Cymbopogon citratus</i> Extracts

“…The results obtained with Cynbopogon citratus extracts were in accordance with those obtained by [12] [24] who showed that these extracts had antifungal activities on the development of Colletotrichum kahawae responsible for anthracnose of coffee berry and Colletotrichum musae and Aspergillus niger responsible for banana fruit rot respectively. Similarly, those obtained with Allium ampeloprasum extract are similar to the results obtained by [25] who showed that the A. sativum and A. ampeloprasum extracts inhibited the development of Alternaria triticina, causal agent of leaf blight in wheat and Magnaporthe oryzae, causal agent of blast disease in rice.…”

“…In addition, the results are also similar to those of [28], reported that Cupressus lusitanica extracts had fungistatic activity on the growth of Phytophtora colocasiae, the agent responsible for taro blight. The fungitoxicity of Cymbopogon citratus extract on the growth of the pathogens was in accordance with that of [25], who showed the fungitoxicity activity of these extracts on the development of Alternaria triticina, causal agent of leaf blight in wheat.…”