Evaluation of antigenotoxicity effects of umbelliprenin on human peripheral lymphocytes exposed to oxidative stress

Soltani, Fatemeh; Mosaffa, Fatemeh; Iranshahi, Mehrdad; Karimi, Gholamreza; Malekaneh, Mohammad; Haghighi, Fatemeh; Behravan, Javad

doi:10.1007/s10565-008-9083-9

Cited by 35 publications

(27 citation statements)

References 21 publications

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“…Umbelliprenin has been shown to be a promising antiinflammatory and anti-oxidant agent in both in vivo and in vitro models (9,10). The induction of apoptosis and chemopreventive activities has also been reported for this compound (12)(13)(14)(15)(16).…”

Section: Discussionmentioning

confidence: 99%

“…MMPs play critical roles in tumor invasion and the inflammatory process (8). Secondly, in vitro and in vivo models have provided evidence of antilipoxygenase, antioxidants; and anti-inflammatory properties for umbelliprenin (9,10). Chronic inflammation and free radicals can accelerate several aspects of tumorigenesis (9,11).…”

Section: Introductionmentioning

confidence: 99%

“…However, umbelliprenin may differently act on the different cell lines of a particular type of cancer (14). Moreover, colon cancer develops in a potentially inflammatory environment (3), and umbelliprenin might still be useful in colon cancer due to its anti-inflammatory properties (9,10). Therefore, its effects on colon cancer cell death and proliferation need to be clarified.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic/Proliferative Effects of Umbelliprenin on Colon Cancer Cell Lines

Hamidinia¹,

Ramezani²,

Mojtahedi³

2013

Ann Colorectal Res

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Background: Colon carcinoma growth depends on many factors such as, different organisms, and immune cells, which induce and produce inflammatory cytokines. Umbelliprenin is a naturally prenylated coumarin with anti-inflammatory activities. Its ability to induce cancer cell death has shown variation on different cancer cell lines. Objectives: 7-Prenyloxycoumarins, including umbelliprenin have been widely investigated because of their pharmacological activities. This paper shows the effect of umbelliprenin on colon cancer cell lines. Material and Methods: In the present study, invasive SW48 and noninvasive SW1116 were treated with umbelliprenin (6.25, 12.5, 25, 50, 100, and 200 μM), and the cytotoxicity was determined using a methyl thiazolelydiphenyl-tetrazolium bromide (MTT) assay. Results: Umbelliprenin had significant cytotoxic activity against SW48 cells at all study concentrations (except for 6.25 μM), with IC50 values of 117, 77, and 69 μM after 24, 48, and 72 h, respectively. However, it was cytotoxic against SW1116 only at higher concentrations of 100 and 200 μM (34% and 64% cell death). At lower concentrations, umbelliprenin showed a significant proliferative effect on this noninvasive cancer cell line. Our data were validated by eye and microscopic images. Conclusions:We found a moderate cytotoxic activity of umbelliprenin against invasive SW48 cells, and both cytotoxic and proliferative effects on noninvasive SW1116 cells. Therefore, using umbelliprenin as an anti-inflammatory or cytotoxic compound for patients with colon cancer should be used with care.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytotoxic/Proliferative Effects of Umbelliprenin on Colon Cancer Cell Lines

Hamidinia¹,

Ramezani²,

Mojtahedi³

2013

Ann Colorectal Res

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“…Umbelliprenin (Umb; C 24 H 30 O 3 ; molecular weight, 366), a member of 7-prenyloxycoumarins, is a naturally occurring compound, which is isolated from Ferula species, including Ferula szowitsiana (1,2). This compound is able to suppress tumorigenesis through its anti-inflammatory, anti-genotoxicity, anti-invasive and lipoxygenase inhibitory activities (3)(4)(5)(6). In vitro, Umb has been reported to attenuate the proliferation of various cancer cell lines, and is able to prevent/delay cancer in in vivo animal models of papilloma and lung cancer (7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

Immune-associated proteins with potential in vivo anti-tumor activities are upregulated in lung cancer cells treated with umbelliprenin: A proteomic approach

et al. 2016

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Abstract. Umbelliprenin (Umb), a natural coumarin, has demonstrated anti-tumor activities, both in vitro and particularly in vivo, in several types of cancer, including lung cancer. The present study aimed to identify molecular targets of Umb using a high-throughput approach. Lung cancer cell lines, QU-DB (large-cell lung carcinoma) and A549 (adenocarcinoma), were treated with Umb. Differentially-expressed proteins were identified using two-dimensional electrophoresis coupled to mass spectrometry. In the QU-DB cells, differential expression of proteins, including downregulation of the tumorigenic protein heat shock protein 90 kDa and upregulation of the potential anti-tumor proteins Nipsnap1 and glycine-tRNA ligase (GRS), suggested that Umb is a strong anti-tumor compound. In the A549 cells, differential expression of proteins indicated possible contradictory effects of Umbregarding tumorigenesis, which included downregulation of the tumorigenic protein cyclophilin and the tumor suppressor MST, and upregulation of stathmin (tumorigenic) and calreticulin. Calreticulun, in addition to GRS in QU-DB cells, stimulates anti-tumor immune responses in vivo. To the best of our knowledge, the present study is the first to use a high-throughput approach to identify targets of Umb in cancer. These molecular targets suggested that Umb may exhibit stronger in vitro anti-tumor activity against the large-cell carcinoma model than the adenocarcinoma model. Furthermore, it has been reported that Umb exhibits higher cytotoxicity against QU-DB cells than A549 cells in vitro, and significant Umb anti-tumor activity against lung cancer in vivo, which is consistent with previously published literature. In each cell type, immune-associated molecules were upregulated, indicating that this naturally occurring compound exhibits marked anti-tumor activity in vivo. However, further studies that investigate the effect of Umb in different in vitro models of cancer are required.

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“…Ascorbic acid (Vitamin C) act as antioxidant and has long been known for its anti-cancer properties (11). The comet assay is a defined genotoxicity assay (in vitro and in vivo) of chemicals (12,13) and it is a simple, rapid, and sensitive method for detecting DNA breaks in individual cells, and is commonly used in genotoxicity assays, primary researches in DNA damage and repair and is also being increasingly more used in human biomonitoring (14,15,16). The aim of the present was to detect the protective effect of AA on H2O2 that induced oxidative damage in DNA by using Comet assay.…”

mentioning

confidence: 99%

Effect of Ascorbic Acid (Vitamin C) on H2O2 Induced Oxidative DNA Damage in Human Lymphocytes Estimated by Comet Assay

Abdullah¹,

Asadi²,

Saleh³

2018

djps

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Abstract"Oxidative DNA damage can be induced by reactive oxygen species and free radicals. Reactive Oxygen Species which is induced by oxidative damage plays a key role in DNA damage". The aim of our study is to identify the protective effect of ascorbic acid (AA, vitamin C) on hydrogen peroxide which induced oxidative damage in DNA by using the Comet assay. Lymphocytes pretreated with or without antioxidants, incubated at 37C for 30 minutes, then H2O2 (100µM) was added & incubated again at 37C for 1 hour (60 minutes). Viability of cells was detected by trypan blue stain exclusion method. The decrease in viability brought about by H2O2 when the cells incubated for 60 minutes and the viability was present to be 39±3% from 80±4% and it was highly developed by the found of AA at 100 μM which appeared 72 ± 1%. These findings indicate that the activity of ascorbic acid as an antioxidant is evidenced by its ability to suppress the oxidative effect against H2O2 and protect the lymphocytes. Estimation of comet tail moment and tail length in human lymphocyte treated with 100µM of hydrogen peroxide as positive control showed that 13± 4.5% of the cells showed no DNA damage while the DNA damage from low to very high damage were 19± 3.5%, 12.2± 2.3%, 14± 3.2% and 37± 3.0%, respectively. In contrast, treatment of the cells with 100 µM H2O2in combination with 10, 25,75

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Evaluation of antigenotoxicity effects of umbelliprenin on human peripheral lymphocytes exposed to oxidative stress

Cited by 35 publications

References 21 publications

Cytotoxic/Proliferative Effects of Umbelliprenin on Colon Cancer Cell Lines

Cytotoxic/Proliferative Effects of Umbelliprenin on Colon Cancer Cell Lines

Immune-associated proteins with potential in vivo anti-tumor activities are upregulated in lung cancer cells treated with umbelliprenin: A proteomic approach

Effect of Ascorbic Acid (Vitamin C) on H2O2 Induced Oxidative DNA Damage in Human Lymphocytes Estimated by Comet Assay

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