Evaluation of Antimicrobial Activity of Four Organic Acids Used in Chicks Feed to ControlSalmonella typhimurium: Suggestion of Amendment in the Search Standard

Abstract

Today, the general public has become increasingly aware of salmonellosis problems. Organic acids are known by their antimicrobial potential and commonly used for improving the quality of poultry feed. In this context, the present work evaluated the inhibitory effect of four organic acids, namely, acetic acid, citric acid, lactic acid, and tartaric acid, at different levels of contamination by Salmonella typhimurium. The neutralization of these organic acids in vitro and in the presence of one-day-old chick's organs was also investigated during the search for Salmonella serovars in birds as described in the Moroccan standard “NM 08.0.550.” The effect of four organic acids on Salmonella typhimurium was tested in vitro and in the presence of chick's organs at different concentrations set of strain and organic acids tested. The MIC results demonstrated that tartaric acid, citric acid, and acetic acid inhibited Salmonella typhimurium at concentrations of 0.312%, 0.625%, and 0.512% for the three levels of strain: 10, 100, and 103 CFU/ml, respectively, while lactic acid and depending on the amount of the strain introduced acts differently: 0.078% for 10 CFU/ml and 0.156% for 100 and 103 CFU/ml. The concentration of 0.04M of Na2HPO4 solution has proved, in vitro, in caecums and organs of chicks (in presence of organic acids) that strain introduced, even at low concentrations, can be recovered. The use of additives has beneficial effects in Salmonella control program. However, the present results recommend the amendment of Salmonella research standard, taking into account the probable presence of organic acids in digestive content of one-day-old chicks.

Role of acidifiers in livestock nutrition and health: A review

“…Broiler fed rations supplemented with organic acids showed a decrease (linear, p = .002) in E. coli populations and increase ( p = .042) in Lactobacillus populations (Nguyen, Lee, Mohammadigheisar, & Kim, ). Use of some organic acids such as tartaric, citric and acetic acids decreased S. t yphimurium at doses of 0.312%, 0.512% and 0.625% for the three levels of strain: 10, 100 and 10 3 CFU/ml respectively (El Baaboua et al, ). The potent role of acidifiers in countering important poultry pathogens including of food‐borne bacterial pathogens and others having public health concerns need to be exploited further for safeguarding poultry health and combating zoonosis (Dhama et al, ; Ramees et al, ).…”

“…typhimurium at doses of 0.312%, 0.512% and 0.625% for the three levels of strain: 10, 100 and 10 3 CFU/ml respectively (El Baaboua et al, 2018). The potent role of acidifiers in countering important poultry pathogens including of food-borne bacterial pathogens and others having public health concerns need to be exploited further for safeguarding poultry health and combating zoonosis (Dhama et al, 2013;Ramees et al, 2017).…”

Research Note: Effect of organic acid mixture on growth performance and Salmonella Typhimurium colonization in broiler chickens

“…Previously, antimicrobial growth promotors ( AGP ) were used to maintain bird health and improvement, but because of its increasing resistance, AGP have been banned or their use discouraged ( Huyghebaert et al., 2011 ). Organic acids are approved and are safe to use in animal as feed additives ( Baaboua et al., 2018 ). Organic acids are used to reduce or eliminate Salmonella in feed ingredients either before heat treatment or supplemented in the feed ( Koyuncu et al., 2013 ).…”

An 1H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus

“…Recently the inhibitory effect of four organic acids (acetic acid, citric acid, lactic acid, and tartaric acid) was evaluated at different levels of contamination by S. typhimurium [ 42 ].…”