Evaluation of antimicrobial and antibiofilm properties of chitosan edible coating with plant extracts against Salmonella and E. coli isolated from chicken

Abstract

This study aims to investigate the effect of chitosan edible coating with medicinal leaf extracts, their antibiofilm and antimicrobial activity against the selected strains of Salmonella spp. and E. coli isolated from the chicken sample. The presence of bioactive compounds in leaf extracts was confirmed by antioxidant assay, polyphenolic content, UV–VIS, FTIR, and GC–MS analysis. Chitosan with M. piperita and P. amboinicus (ECMO) had a greater zone of inhibition against the multidrug‐resistant strains (Salmonella spp., 22.10 ± 1.15; E. coli, 24.50 ± 0.500) and biofilm inhibition percentage was ≤96% in both Salmonella spp. and E. coli. In shelf‐life analysis, ECMO significantly lowered the growth of pathogens during 15 days of storage period. The results suggest that ECMO was identified as the impeding agent for biofilms and foodborne pathogens. To our knowledge, this is the first study demonstrating the antibiofilm and antibacterial properties of chitosan with M. piperita and P. amboinicus. Novelty impact statement The combination of chitosan with M. piperita and P. amboinicus is the first study on chicken that significantly controlled pathogens during storage period at 4°C. Antibiofilm property was exhibited by chitosan with M. piperita and P. amboinicus against Salmonella spp. and E. coli. Chitosan with M. piperita and P. amboinicus, perchance a new natural preservative for perishable foods.

Overview of plant extracts as natural preservatives in meat

“…Chitosan edible film infused with extract of medicinal plants peppermint ( Mentha piperita ) and Indian borage ( Plectranthus amboinicus ) was observed to exert antibiofilm (≤96%) and antimicrobial activity against multidrug‐resistant strains E. coli (zone of inhibition 24.50 ± 0.500) and Salmonella spps (22.10 ± 1.15) isolated from chicken meat (Johnson et al, 2022). The chicken meat packaged in extract‐infused chitosan active films has significantly reduced the growth of pathogenic bacteria during 15 days of refrigerated storage (Johnson et al, 2022). Erken et al (2022) incorporated Prunella leaves extracts in chitosan active films with comparative physical and surface properties.…”

Natural Antimicrobials: A Clean Label Strategy to Improve the Shelf Life and Safety of Reformulated Meat Products

“…In packaged chicken meat, chitosan edible film with extracts of peppermint and Indian borage has bactericidal and antibiofilm activity against E. coli (>2.5 log reductions) and Salmonella spp. (~3 log reductions), and both active films inhibit the growth of both pathogens during refrigerated storage (15 days) [ 139 ].…”