Evaluation of Antimicrobial-Antibiofilm Activity of a Hydrogen Peroxide Decontaminating System Used in Dental Unit Water Lines

Orrù, Germano; Nero, Susanna Del; Tuveri, Enrica; Ciusa, Maria Laura; Pilia, Francesca; Erriu, Matteo; Orrù, Ginevra; Liciardi, Manuele; Piras, Vincenzo; Denotti, Gloria

doi:10.2174/1874210601004010140

Cited by 24 publications

(24 citation statements)

References 21 publications

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“…However, we were able to effectively eradicate the bacteria within a preformed biofilm. Our results differ from previous works evaluating the same agents to disrupt preformed biofilms of environmental and human bacterial isolates (51)(52)(53)(54)(55)(56)(57)64). In the current study, preformed biofilm biomass was reduced by Ͼ80%, and the mean log of CFU was decreased by Ͼ3 log in the laboratory control strains of E. coli, P. aeruginosa, and K. pneumoniae.…”

Section: Figcontrasting

confidence: 99%

“…The resistance of P. aeruginosa to hydrogen peroxide is mediated through the production of catalase enzyme that inactivates hydrogen peroxide (56). Strains of E. coli associated with dental hardware are relatively susceptible to disruption of a preformed biofilm and killing with hydrogen peroxide (57), and a similar effect was observed in the current study, as E. coli preformed biofilms were relatively easy to disrupt and kill the bacteria residing within the biofilm. It should also be noted that the in vitro effects of hydrogen peroxide observed in the current study might not be observed in vivo due to inactivation from catalases produced by host tissues (58).…”

Section: Figsupporting

confidence: 78%

See 1 more Smart Citation

In Vitro Efficacy of Nonantibiotic Treatments on Biofilm Disruption of Gram-Negative Pathogens and an In Vivo Model of Infectious Endometritis Utilizing Isolates from the Equine Uterus

et al. 2016

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In this study, we evaluated the ability of the equine clinical treatments N-acetylcysteine, EDTA, and hydrogen peroxide to disrupt in vitro biofilms and kill equine reproductive pathogens (Escherichia coli, Pseudomonas aeruginosa, or Klebsiella pneumoniae) isolated from clinical cases. N-acetylcysteine (3.3%) decreased biofilm biomass and killed bacteria within the biofilms of E. coli isolates. The CFU of recoverable P. aeruginosa and K. pneumoniae isolates were decreased, but the biofilm biomass was unchanged. Exposure to hydrogen peroxide (1%) decreased the biofilm biomass and reduced the CFU of E. coli isolates, K. pneumoniae isolates were observed to have a reduction in CFU, and minimal effects were observed for P. aeruginosa isolates. Chelating agents (EDTA formulations) reduced E. coli CFU but were ineffective at disrupting preformed biofilms or decreasing the CFU of P. aeruginosa and K. pneumoniae within a biofilm. No single nonantibiotic treatment commonly used in equine veterinary practice was able to reduce the CFU and biofilm biomass of all three Gram-negative species of bacteria evaluated. An in vivo equine model of infectious endometritis was also developed to monitor biofilm formation, utilizing bioluminescence imaging with equine P. aeruginosa isolates from this study. Following infection, the endometrial surface contained focal areas of bacterial growth encased in a strongly adherent "biofilm-like" matrix, suggesting that biofilms are present during clinical cases of infectious equine endometritis. Our results indicate that Gram-negative bacteria isolated from the equine uterus are capable of producing a biofilm in vitro, and P. aeruginosa is capable of producing biofilm-like material in vivo. Bacterial endometritis is an important cause of subfertility in mares (1). Endometrial infections are reported in 25 to 60% of mares who fail to become pregnant following breeding (1), contributing to a major economic loss for the equine industry (1, 2). The most common species of bacteria identified during the clinical diagnosis of bacterial endometritis include Streptococcus equi subsp. zooepidemicus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa (3, 4). The isolation of one of these species of bacteria is considered to be clinically relevant due to observed reductions in pregnancy rates after identification (2). The detection of these bacteria in the uterus would result in treatment with uterine lavage and broad-spectrum antibiotics to reduce bacterial load and eradicate the remaining bacteria (5, 6).Bacterial endometritis that is refractory to traditional antimicrobial treatment is a significant challenge in the equine breeding industry (4). One possible explanation often cited for the failure of antibiotic treatment is the growth of bacterial pathogens in a biofilm (1, 5). Uterine isolates of E. coli, P. aeruginosa, and K. pneumoniae have been proposed to most likely be associated with a biofilm, due to the observation of repeated antibiotic treatment failures in equine reproducti...

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Section: Figcontrasting

confidence: 99%

Section: Figsupporting

confidence: 78%

In Vitro Efficacy of Nonantibiotic Treatments on Biofilm Disruption of Gram-Negative Pathogens and an In Vivo Model of Infectious Endometritis Utilizing Isolates from the Equine Uterus

et al. 2016

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“…The disinfectant effectiveness of apple vinegar and hydrogen peroxide found in this research corroborates with the studies of Perez et al . and Orrù et al ,. especially considering the reduction of E. coli viability with hydrogen peroxide treatment.…”

Section: Discussionsupporting

confidence: 93%

“…In view of low toxicity, low cost and a viable home-use application, apple vinegar and hydrogen peroxide showed the best antimicrobial activity against reference strains in screening tests (agar well diffusion and MIC/MMC evaluation). Vinegar and hydrogen peroxide have been suggested as interesting alternatives for the disinfection of other materials 19,20,[25][26][27][28][29] .…”

Section: Discussionmentioning

confidence: 99%

Control of bacterial biofilms formed on pacifiers by antimicrobial solutions in spray

Pedroso

Sangalli

Brighenti

et al. 2018

Int J Paed Dentistry

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“…Chlorhexidine 13,14,34) , tetraacetyl enediamine 38) , hydrogen peroxide 24,33) , strong-type acidic electrolyzed water 20) , bleach, and other chemicals 27) have been used in dental unit waterline decontamination. In our study, we found MRSA and P. aeruginosa in the control DUWS by PCR.…”

Section: Discussionmentioning

confidence: 99%

Antiseptic Effect of Slightly Acidic Electrolyzed Water on Dental Unit Water Systems

Komachiya

Yamaguchi²,

Hirai³

et al. 2014

Bull. Tokyo Dent. Coll.

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Biofilm formation in dental unit water systems (DUWSs) can contaminate water from three-in-one syringes, air rotors, and low-speed handpieces. This may serve as a potential source of infection for dentists, dental staff, and patients, so these systems must be sterilized. Because slightly acidic electrolyzed water (SAEW) is often used as a disinfectant for food, the aim of this study was to investigate the possibility of using SAEW as a DUWS disinfectant. Slightly acidic electrolyzed water was injected into a dental unit and its effects evaluated. Chemical properties such as chlorine ion and potential hydrogen in the SAEW were measured. Detection of both ordinary and heterotrophic bacteria from the DUWS was performed by culture, and biofilm formation of the bacteria in the DUWS evaluated. Polymerase chain reaction (PCR) was used to detected contamination by nosocomial pathogens. Almost all the chlorine ions in the SAEW were exhausted during the two-day trials, and the pH value of the SAEW fell from 5 to 4. No viable cells were detected in the SAEW collected. Biofilm formation in the water from the DUWS with SAEW was almost at a baseline level, whereas that without SAEW was 4 times higher. The PCR analysis showed that no nosocomial infecting pathogens were detected in the SAEW. The present study demonstrated the antiseptic effect of SAEW in DUWS.

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Evaluation of Antimicrobial-Antibiofilm Activity of a Hydrogen Peroxide Decontaminating System Used in Dental Unit Water Lines

Cited by 24 publications

References 21 publications

In Vitro Efficacy of Nonantibiotic Treatments on Biofilm Disruption of Gram-Negative Pathogens and an In Vivo Model of Infectious Endometritis Utilizing Isolates from the Equine Uterus

In Vitro Efficacy of Nonantibiotic Treatments on Biofilm Disruption of Gram-Negative Pathogens and an In Vivo Model of Infectious Endometritis Utilizing Isolates from the Equine Uterus

Control of bacterial biofilms formed on pacifiers by antimicrobial solutions in spray

Antiseptic Effect of Slightly Acidic Electrolyzed Water on Dental Unit Water Systems

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