Evaluation of antimicrobial, antioxidant and phototoxic activities of extracts and isolated compounds from Stachytarpheta cayennensis (Rich.) Vahl, Verbenaceae

Souza, Pierre André de; Silva, Carmelita Gomes; Machado, Barbara Rosa Pimentel; Lucas, Nanci C. de; Leitão, Gilda Guimarães; Eleuthério, Elis C. A.; Ortiz, Gisela Maria Dellamora; Benchetrit, Leslie C.

doi:10.1590/s0102-695x2010005000042

Cited by 16 publications

(6 citation statements)

References 29 publications

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“…Vo-C contains a concentration of verbascoside and isoverbascoside superimposable to Vo-S but about half the amount of phenolic acids, which could explain the lower antioxidant efficacy observed for the extract. These findings suggest that the antioxidant ability of the extracts may be attributed both to the phenylpropanoid glycosides and to the phenolic acids, whose antioxidant properties have been previously demonstrated [48][49][50][51].…”

Section: Biological Activitiessupporting

confidence: 59%

Production of Verbascoside, Isoverbascoside and Phenolic Acids in Callus, Suspension, and Bioreactor Cultures of Verbena officinalis and Biological Properties of Biomass Extracts

et al. 2020

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Callus, suspension and bioreactor cultures of Verbena officinalis were established, and optimized for biomass growth and production of phenylpropanoid glycosides, phenolic acids, flavonoids and iridoids. All types of cultures were maintained on/in the Murashige and Skoog (MS) media with 1 mg/L BAP and 1 mg/L NAA. The inoculum sizes were optimized in callus and suspension cultures. Moreover, the growth of the culture in two different types of bioreactors—a balloon bioreactor (BB) and a stirred-tank bioreactor (STB) was tested. In methanolic extracts from biomass of all types of in vitro cultures the presence of the same metabolites—verbascoside, isoverbascoside, and six phenolic acids: protocatechuic, chlorogenic, vanillic, caffeic, ferulic and rosmarinic acids was confirmed and quantified by the HPLC-DAD method. In the extracts from lyophilized culture media, no metabolites were found. The main metabolites in biomass extracts were verbascoside and isoverbascoside. Their maximum amounts in g/100 g DW (dry weight) in the tested types of cultures were as follow: 7.25 and 0.61 (callus), 7.06 and 0.48 (suspension), 7.69 and 0.31 (BB), 9.18 and 0.34 (STB). The amounts of phenolic acids were many times lower, max. total content reached of 26.90, 50.72, 19.88, and 36.78 mg/100 g DW, respectively. The highest content of verbascoside and also a high content of isoverbascoside obtained in STB (stirred-tank bioreactor) were 5.3 and 7.8 times higher than in extracts from overground parts of the parent plant. In the extracts from parent plant two iridoids—verbenalin and hastatoside, were also abundant. All investigated biomass extracts and the extracts from parent plant showed the antiproliferative, antioxidant and antibacterial activities. The strongest activities were documented for the cultures maintained in STB. We propose extracts from in vitro cultured biomass of vervain, especially from STB, as a rich source of bioactive metabolites with antiproliferative, antioxidant and antibacterial properties.

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Section: Biological Activitiessupporting

confidence: 59%

Production of Verbascoside, Isoverbascoside and Phenolic Acids in Callus, Suspension, and Bioreactor Cultures of Verbena officinalis and Biological Properties of Biomass Extracts

et al. 2020

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“…However, the phototoxic effects of betulinic acid had been previously evaluated, and no photosensitizing effects were reported for betulinic acid extracted from Stachytarpheta cayennensis (Rich.) Vahl, Verbenaceae [66]. Moreover, there is the possibility of light reactions with some of the flavonoids in the extract.…”

Section: Discussionmentioning

confidence: 99%

Study of the potential adverse effects caused by the dermal application of Dillenia indica L. fruit extract standardized to betulinic acid in rodents

et al. 2019

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This study aimed to evaluate the potential adverse effects of the dermal administration of Dillenia indica Linnaeus ( D . indica ) fruit extract in healthy rodents; the extract was standardized to betulinic acid. In the initial phase, the acute effects were evaluated on the skin application site of a single extract dose. A skin irritation test was performed in male Wistar rats (n = 8/group) receiving the extract (50–150 mg/mL) with betulinic acid (0.5–1.5%, respectively). A photosensitivity test was performed in male BALB/c mice (n = 6/group) receiving the extract (150 mg/mL). Afterwards, other BALB/c mice (n = 20, male:female, 1:1) were used to assess the systemic alterations caused by 14 daily repeated doses (150 mg/mL) by monitoring the effects on mortality, body morphology, behavior, nutrition status, neuromotor reactions, organ morphology and weight, and blood tests. At this time, 0.5 mg/mL clobetasol was used as the positive control. The skin irritation index suggested that negligible skin irritation had occurred, even when the extract was applied to the rat skin at 150 mg/mL. However, the extract acted as a photosensitizer on mouse skin, showing a photosensitizing activity close to that of 10 mg/mL 5-methoxypsoralen. Repeated doses caused no mouse mortality, aggressiveness, piloerection, diarrhea, convulsions, neuromotor alterations or nutrition status changes. The mouse organ weights did not change, and the mice did not have alterations in their blood compositions. Clobetasol caused a reduction in the mononuclear leukocyte numbers. In general, the data suggest that the extract was safe in healthy rodents but indicate that caution should be taken with the photosensitizing activity; in addition, this activity should be further explored as it may be useful for phototherapeutic drug development.

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“…The verbascoside (2), martinoside (3) and betulinic acid (4) resulting from the roots of ethanol extract of S.cayennensis has been previously assayed by our group for antioxidant activities (DPPH freeradical assay and Saccharomyces cerevisiae cells, an eukaryotic cell model) (De Souza et al, 2010). All compounds afforded the best results in the two assays and were therefore, chosen for this study.…”

Section: Antioxidant Activity Of Natural Compounds Of Stachytarpheta mentioning

confidence: 99%

Antioxidant activity of natural compounds of Stachytarpheta cayennensis (Rich.) Vahl, Verbenaceae, by scavenger of mitochondrial reactive oxygen species

Souza¹,

Rodrigues²,

Santiago³

et al. 2011

Rev. bras. farmacogn.

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Abstract:The etiology of a range of diseases is associated with an excessive generation of reactive oxygen species (ROS). Exacerbated ROS production leads to mitochondrial dysfunction, cellular damage and apoptosis. Nowadays, many strategies are being developed for the targeted delivery of antioxidants compounds to mitochondria, trying to minimize the damages. Production of ROS was investigated by the molecular probes CM-H 2 DCFDA and Amplex Red. The purpose of this work is to evaluate the antioxidant capacity of verbascoside, martinoside, betulinic acid from the Stachytarpheta cayennensis and quercetin by an in vitro assay with isolated mitochondria from mice's brain The results showed that all compounds tested exhibited a scavenger effect on the ROS generated by the isolated mitochondria, which displayed a dependent dose increase.

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Evaluation of antimicrobial, antioxidant and phototoxic activities of extracts and isolated compounds from Stachytarpheta cayennensis (Rich.) Vahl, Verbenaceae

Cited by 16 publications

References 29 publications

Production of Verbascoside, Isoverbascoside and Phenolic Acids in Callus, Suspension, and Bioreactor Cultures of Verbena officinalis and Biological Properties of Biomass Extracts

Production of Verbascoside, Isoverbascoside and Phenolic Acids in Callus, Suspension, and Bioreactor Cultures of Verbena officinalis and Biological Properties of Biomass Extracts

Study of the potential adverse effects caused by the dermal application of Dillenia indica L. fruit extract standardized to betulinic acid in rodents

Antioxidant activity of natural compounds of Stachytarpheta cayennensis (Rich.) Vahl, Verbenaceae, by scavenger of mitochondrial reactive oxygen species

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