Evaluation of antioxidant activity and phenolic profile of filamentous terrestrial cyanobacterial strains isolated from forest ecosystem

Babić, Olivera; Kovač, Dajana; Rašeta, Milena; Šibul, Filip; Svirčev, Zorica; Simeunović, Jelica

doi:10.1007/s10811-015-0773-4

Cited by 41 publications

(17 citation statements)

References 44 publications

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“…Phenolic acids and flavonoids from plant sources have been well-documented for their antioxidant, free-radical quenching and redox metal ion-chelating affects (Singh et al 2009(Singh et al , 2014Tutour 1990;Apati et al 2003;Hossain et al 2016) but cyanobacteria and microalgae are relatively new source for such compounds in recent years due to their potential benefits and ease of production (Guedes et al 2013;Machu et al 2015). Gallic, chlorogenic, caffeic and ferulic acids identified from plant sources have been known as strong antioxidants and free radical scavengers (Piazzon et al 2012;Babić et al 2015) but their presence in cyanobacteria is scarcely reported and this make these organisms more potential. Being most primitive, these organisms are considered to evolve with various levels of environmental adaptations to overcome these stresses for their survival over the time (Tandeau-de-Marsac and Houmard 1993).…”

Section: Resultsmentioning

confidence: 99%

Antioxidant properties and polyphenolic content in terrestrial cyanobacteria

et al. 2017

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Cell-free extracts of twenty terrestrial cyanobacteria were evaluated for their antioxidant properties in terms of free-radical scavenging (DPPH and ABTS) and metal chelating activity and deoxyribose protection. Extract of Anabaena constricta was the most prominent antioxidant agent (IC 50 for DPPH activity 0.91 mg ml -1 , ABTS 0.23 mg ml -1 , deoxyribose protection 0.63 mg ml -1 and Fe ?2 -ion chelating 0.9 mg ml -1 ). The extracts of cyanobacterial species contained high quantity of total phenol and total flavonoid that were supposed to impart prominent antioxidant properties. Cyanobacterial species also showed fairly high PAL activity. We reported varied quantities of polyphenolics gallic, chlorogenic, caffeic, vanillic and ferulic acids and flavonoids rutin, quercetin and kaempferol in cyanobacterial extracts. The presence of these polyphenolics was linked with the free radical scavenging, metal chelating and antioxidative damage protecting properties of the organisms. Cyanobacteria are the most feasible, promising and alternative candidates for searching out new chemical leads for industrial applications in pharmaceuticals, neutraceuticals and biomolecules of importance. Presence of biomolecules such as polyphenolics and their connection with the prominent biological functions (e.g., antioxidant properties) make these organisms a potential source of secondary metabolites with predominant biological activities. Additionally, dominant presence of polyphenols as antioxidant agents in cyanobacterial species may reflect their adaptation strategies against abiotic stresses for their ecological success in different habitats.

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Section: Resultsmentioning

confidence: 99%

Antioxidant properties and polyphenolic content in terrestrial cyanobacteria

et al. 2017

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“…Weak negative correlations between the antioxidant capacity and TPC were also reported in previous studies [ 35 ]. Consequently, it can be inferred that crude extracts might not only provide carotenoids and phenols, but a complex mixture of compounds that may also have antioxidant potential, such as fatty acids, exopolymers and phycocyanin [ 43 , 44 ]. The results obtained herein with the strain Phormidium sp.…”

Section: Discussionmentioning

confidence: 99%

Exploitation of Filamentous and Picoplanktonic Cyanobacteria for Cosmetic Applications: Potential to Improve Skin Structure and Preserve Dermal Matrix Components

et al. 2020

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The use of natural products in skin care formulations gained interest as a concern for modern societies. The undesirable side effects of synthetic COMPOUNDS, as well as the associated environmental hazards, have driven investigation on photosynthetic organisms as sustainable sources of effective and environmentally friendly ingredients. The use of natural extracts in cosmetics has been highlighted and, along with plants and algae, cyanobacteria have come into focus. Due to their low culture demands, high grow rates and ability to produce a wide variability of bioactive metabolites, cyanobacteria emerged as an economic and sustainable base for the cosmetic industry. In this study, we evaluated the potential of ethanol extracts of picocyanobacteria strains of the genera Cyanobium and Synechocystis and filamentous strains of the genera Nodosilinea, Phormidium and Tychonema for skin applications, with focus in the field of anti-aging. The extracts were analyzed for their pigment profile, phenolic content, antioxidant potential, cytotoxicity against keratinocytes (HaCat), fibroblasts (3T3L1), endothelial cells (hCMEC/D3) and capacity to inhibit hyaluronidase (HAase). The total carotenoid content ranged from 118.69 to 383.89 μg g−1 of dry biomass, and the total phenolic content from 1.07 to 2.45 mg GAE g−1. Identified carotenoids consisted of zeaxanthin, lutein, canthaxanthin, echinenone and β-carotene, with zeaxanthin and lutein being the most representative (49.82 and 79.08 μg g−1, respectively). The highest antioxidant potential was found for Phormidium sp. LEGE 05292 and Tychonema sp. LEGE 07196 for superoxide anion radical (O2•−) scavenging (IC50 of 822.70 and 924 μg mL−1, respectively). Low or no cytotoxicity was registered. Regarding HAase inhibition, Tychonema sp. LEGE 07196 and Cyanobium sp. LEGE 07175 showed the best IC50 (182.74 and 208.36 μg mL−1, respectively). In addition, an increase in fibroblast proliferation was registered with these same strains. From this work, the ethanol extracts of the species Tychonema sp. and Cyanobium sp. are particularly interesting for their potential application in anti-aging formulations, once they stimulated fibroblast proliferation and inhibit hyaluronic acid digestion.

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“…The production of a wide range of secondary metabolites is a common adaptation of cyanobacteria to compete successfully in different ecosystems. Because of their phototrophic lifestyle and constant exposure to high oxygen and radical stresses, these biota have a high capability for producing plentiful efficient protective chemicals against oxidative and radical stressors and exhibit adaptive responses to oxidative stresses by stimulating their intrinsic antioxidant defence systems based on mediator compounds, including polyphenolic substances (Babić et al 2016 ). Previously published data indicate that the phenolic acids and flavonoids in cyanobacterial cells may be responsible for their antioxidant properties (Singh et al 2017a ), and thus a higher content of phenolics in cyanobacterial species may be presumed as an adaptation strategy of these organisms against abiotic stresses in their specific habitats.…”

Section: Discussionmentioning

confidence: 99%

Biocatalytic hydrogenation of the C=C bond in the enone unit of hydroxylated chalcones—process arising from cyanobacterial adaptations

Żyszka-Haberecht

Poliwoda

Lipok

2018

Appl Microbiol Biotechnol

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To verify the hypothesis that cyanobacteria naturally biosynthesising polyphenolic compounds possess an active enzymatic system that enables them to transform these substances, such an ability of the biocatalytic systems of whole cells of these biota was assessed for the first time. One halophilic strain and seven freshwater strains of cyanobacteria representing four of the five taxonomic orders of Cyanophyta were examined to determine the following: (i) whether they contain polyphenols, including flavonoids; (ii) the resistance of their cultures when suppressed by the presence of exogenous hydroxychalcones—precursors of flavonoid biosynthesis and (iii) whether these photoautotrophs can transform hydroxylated chalcones. All examined strains were found to contain polyphenols and flavonoids, and the growth of their cultures was inhibited in the presence of 2′-hydroxychalcone, 2″-hydroxychalcone and 4″-hydroxychalcone. We also confirmed that the examined cyanobacteria transformed hydroxychalcones via hydrogenative bio-reduction and formed the corresponding hydroxydihydro derivatives with yields above 90% whenever the substrates were bioavailable for such a conversion. Moreover, we observed that the routes and efficiency of biohydrogenation (and hydroxylation) of chalcones were dependent on the location of the hydroxyl substituent. The final products obtained as the results of biotransformations were extracted from the media and identified by mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (1H NMR, 13C NMR, COSY, HSQC). Based on those results, we believe that the very efficient biohydrogenation of hydroxychalcones, which may easy be scaled up for biotechnological purposes, reflects the natural activity of the cyanobacterial defence system, because hydroxydihydrochalcones were less active inhibitors of the growth of cyanobacterial cultures than the corresponding substrates.

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Evaluation of antioxidant activity and phenolic profile of filamentous terrestrial cyanobacterial strains isolated from forest ecosystem

Cited by 41 publications

References 44 publications

Antioxidant properties and polyphenolic content in terrestrial cyanobacteria

Antioxidant properties and polyphenolic content in terrestrial cyanobacteria

Exploitation of Filamentous and Picoplanktonic Cyanobacteria for Cosmetic Applications: Potential to Improve Skin Structure and Preserve Dermal Matrix Components

Biocatalytic hydrogenation of the C=C bond in the enone unit of hydroxylated chalcones—process arising from cyanobacterial adaptations

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