Evaluation of antioxidant activity of hesperidin and its protective effect on H2O2 induced oxidative damage on pBR322 DNA and RBC cellular membrane

Abstract

The present work was carried out to evaluate the antioxidant activity of hesperidin and to study its protective effect on H(2)O(2) induced oxidative damage on pBR322 DNA and RBC cellular membrane. The in vitro assays were performed with different concentrations (2, 4, 6, 8, and 10 microg/ml, which were equivalent to 3.27, 6.55, 9.83, 13.10, and 16.38 microM) of hesperidin and the results clearly indicate that hesperidin at 10 microg/ml exhibited radical scavenging activity greater than that of standards like ascorbic acid and trolox. The protective effect of hesperidin on pBR322 DNA and RBC cellular membrane on treatment with different concentrations of H(2)O(2) shows that hesperidin at 2.5 mM converts the open circular form (oc) of pBR322 DNA that is an indication of damage to super coiled (ccc) form and at 10 microg/ml it prevents membrane damage. Thus, our result proves hesperidin to be a valuable antioxidant that protects pBR322 DNA and RBC cellular membrane from free radical induced oxidative damage.

Potential of morin and hesperidin in the prevention of cisplatin-induced nephrotoxicity

“…In addition, hesperidin administration increased antioxidant capacity. 26 Although there have been many studies on the effects of various flavonoids administration on nephrotoxicity, effects of morin þ hesperidin combinations have not been studied yet. The present study was designed to explore the potential protective effects of morin and hesperidin on oxidative stress in cisplatin-induced nephrotoxicity.…”

Hesperidin Reduces Cisplatin-Induced DNA Damage in Bone Marrow Cells of Mice

“…The oxidative stress can alter lipids, proteins and nucleic acids by producing oxidized purines and pyrimidines, single strand breaks and alkali labile sites which may results in direct DNA damage [15,16]. To avoid these toxic side effects, it is common that the used of chemotherapeutic drugs in combination with various protective and detoxifying agents is to eliminate or reduce its adverse toxic effects.…”

“…Previous researches demonstrated its antimutagenic effect against the damage induced by cyclophosphamide [25], an alkylating drug that is activated by cytochrome P-450 in the liver [28,29] and powerful protective effects on the radiation-induced DNA damage in mouse bone marrow cells [30]. Kalpana et al [16] demonstrated its protective effect on H 2 O 2 induced oxidative damage on pBR322 DNA and RBC (red ball cells) cellular membrane. Until today, there have been no studies reporting the antimutagenic potential of hesperidin against the damage induced by cisplatin.…”

Phytochemical investigation and radical scavenging activities ofMelia azedarachand its DNA protective effect in cultured lymphocytes

“…Nitric oxide or reactive nitrogen species, formed during their reaction with oxygen or with superoxides, such as NO 2 , N 2 O 4 , N 3 O 4 , NO À 3 and NO À 2 are very reactive. These compounds are responsible for altering the structural and functional behavior of many cellular components (Kalpana et al, 2009). The nitric oxide radical scavenging ability of the plant extract is due to the presence of squalene present in the ethanol extract of MA.…”

“…An increase in the levels of lipid peroxidation products such as MDA, hydroperoxides and conjugated dienes are the indices of membrane lipid damage. The mechanisms involved in many human diseases such as hepatotoxicites, hepatocarcinogenesis, diabetes, acute myocardial infarction and skin cancer include lipid peroxidation as a main source of membrane damage (Kalpana et al, 2009). Hence, the mechanistic study of membrane damage induced by ROS in relation to human diseases and their possible prevention by antioxidants constitutes an active area of research in recent years (Adhikari et al, 2011).…”