Evaluation of antioxidant and anti-collagenase activity of Rosa damascena L. flower petal and receptacle extract

Abstract

Background: Rosa damascena L. is the most notable species of the Rosaceae family in the world, and has been used in food, cosmetics, and the pharmaceutical industry. Bioactive compounds in this flower are known to have several activities, such as antioxidant, antimicrobial, and anti-inflammatory. In this study, the antioxidant and collagenase inhibitory activities of R. damascena L. petal and receptacle extracts were evaluated. Methods: Ethanolic extraction of R. damascena L. petals (EERP) and R. damascena L. receptacles (EERR) were obtained, and bioactive compounds (flavonoids, phenolics, alkaloids, steroids, tannins, terpenoids, and triterpenoids) were classified by phytochemical screening. Antioxidant activities were analyzed by Ferric Reducing Antioxidant Power (FRAP) assay, while anti-collagenase analysis was examined through the inhibition of collagenase. Results: Phytochemical test revealed the presence of flavonoids, phenolics, alkaloids, steroids, triterpenoids, triterpenes, and tannin. EERP showed higher FRAP activity (164.23 ± 1.34 μM Fe(II)) than EERR (12.85 ± 6.19 μM Fe(II)). EERP also had higher inhibitory activity of collagenase (IC50 = 115.48±1.78 µM/mL) compared to EERR (IC50 = 141.96±6.13 µM/mL). Conclusions: R. damascena L. petal and receptacle ethanol extracts contain several components, such as phenolics, flavonoids, alkaloids, tannins, terpenes, triterpenoids, and steroids. These extracts exhibit antioxidant activity and collagenase inhibition. R. damascena L. petal extract showed higher antioxidant activity through FRAP assay and inhibitory activity of collagenase than R. damascena L. receptacle extract.

Potentials of rose (Rosa damascena) petals and receptacles extract as antioxidant and antihyaluronidase

“…According to Boskabady et al (2011), secondary metabolite compounds in R. damascena include flavonoids and terpenoids. According to Elfitriani et al (2020), RPE and RRE contained flavonoids, phenolics, tannins, and alkaloids. In this study, total phenolic and flavonoid content were examined.…”

Unveiling the mechanisms for the development of rosehip-based dermatological products: an updated review

“…Furthermore, the expanded flowers extract demonstrated marked in vitro anti-collagenase activity (80.6%) in a dose-dependent manner (IC 50 = 47.9 μg/mL), compared to the un-expanded flowers extract (IC 50 = 241.9 μg/mL), which may be attributed to the enriched levels in esters and phenolic ethers (i.e., eugenol and methyleugenol) ( Mohsen et al, 2020 ). Recently, the use of ethanolic extracts of R. damascena petals proved to inhibit collagenase in a dose-dependent manner (IC 50 = 115.4 μM/mL), which was closely followed by an etahnolic extracts of R. damascena receptacles (IC 50 = 141.9 μM/mL) ( Elfitriani et al, 2020 ). This is also evidenced by previous studies, in which R. damascena extract suppressed AP-1 activity that affected metalloproteinase transcription.…”

Phenolic Composition, Antioxidant and Antibacterial Activities of Extract from Flowers of Rosa damascena from Morocco

“…The antioxidant capacity of the ethanolic extracts of two parts of the plant was studied using the same method; FRAP activity was found to be higher for the ethanolic extracts from Rosa damascena petals (164.23 ± 1.34 μM Fe(II)) than receptacles (12.85 ± 6.19 μM Fe(II)) [43]. Dudonné et al reported a ferric reducing power of the aqueous extract of Rosa The antioxidant capacity of the ethanolic extracts of two parts of the plant was studied using the same method; FRAP activity was found to be higher for the ethanolic extracts from Rosa damascena petals (164.23 ± 1.34 µM Fe(II)) than receptacles (12.85 ± 6.19 µM Fe(II)) [43]. Dudonné et al reported a ferric reducing power of the aqueous extract of Rosa damascena in the order of 5.08 ± 0.07 mmol Fe 2+ /g [33].…”