Evaluation of Antioxidant and Hepatoprotective Effects of Dendrophthoe pentranda Leaves on CCl4 -Induced Hepatotoxic Rat

Abstract

The aim of the study was to evaluate the phytochemical profile, antioxidant and hepatoprotective effects of aqueous (ADPL), ethanol (EDPL), chloroform (CDPL) and petroleum ether (PDPL) extracts of Dendrophthoe pentandra leaves. The total phenolic (TPC) and flavonoid contents (TFC) were determined by using Folin Ciocalteu method and aluminium chloride assay, respectively. Antioxidant effect was assessed by using DPPH radical scavenging, ferric reducing power (FRP) and total antioxidant content (TAC) assay. Hepatoprotective effect was evaluated against CCl 4 -induced liver toxicity in long evans rats. Among the extracts, EDPL and PDPL had the highest TAC and TFC. EDPL and PDPL showed potent antioxidant effect that exhibited IC 50 31.62 ± 4.10 and 24.30 ± 3.45 µg/ml (DPPH), EC 50 28.18 ± 4.24 and 39.25 ± 5.20 µg/ml (FRP) and 373.83 ± 9.10, 352.67 ± 10.23, mg/g equivalent of ascorbic acid (TAC), respectively. The CCl 4 significantly induced elevation of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total protein (TP) and bilirubin as well as liver damage (cirrosis, ballooning and necrosis). However, treatment with D. pentandra extracts (200 mg/kg, p.o) significantly counter balanced the toxicity towards the normal animals. The research may direct further advanced studies to find out hepatoprotective compounds.

The protective effects of nerol to prevent the toxicity of carbon tetrachloride to the liver in Sprague-Dawley rats

“…The oxido-reductase enzyme LDH converts pyruvate and lactate in the liver and other physiological tissues. Serum LDH, similar to other hepatic enzymes, exhibits an elevation in cases of hepatic failure, making it a valuable biomarker for assessing the extent of hepatotoxicity [ 31 ]. GGT, a microsomal enzyme, is one of the strongest biomarkers of hepatic disease [ 32 ].…”