Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds

Iamsaard, Sitthichai; Arun, Supatcharee; Burawat, Jaturon; Yannasithinon, Supataechasit; Tongpan, Saranya; Bunsueb, Sudtida; Lapyuneyong, Natthapol; Choowong-In, Pannawat; Tangsrisakda, Nareelak; Chaimontri, Chadaporn; Sukhorum, Wannisa

doi:10.1016/j.joim.2020.03.003

Cited by 18 publications

(34 citation statements)

References 34 publications

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“…TyrPho proteins are responsible for male fertility functions such as spermatogenesis, sperm capacitation and acrosome reaction. The alteration of such protein expression in this study is similar to many reports showing the effects of plant extract on TyrPho protein changes (Arun et al., 2018; Iamsaard et al., 2013, 2020), antioxidant activity of M. charantia extract and its preventive effect on testicular damage in rats treated with valproic acid (Maneenin et al., 2018); protective effect of M. cochinchinensis (L.) Spreng Aril extract on the essential markers in rat testis induced with valproic acid (Iamsaard et al., 2017); antioxidant and hypoglycaemic effects of M. cochinchinensis Spreng. (Gac) Aril extract on reproductive damages in hyperglycaemia mice (Sampannang et al., 2017).…”

Section: Discussionsupporting

confidence: 92%

The effect of Dolichandrone serrulata (wall. ex DC.) Seem. flower extract containing antioxidant capacity and terpenoids on the male reproductive system

et al. 2021

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Although the fruit extract of Dolichandrone genus was shown to inhibit spermatogenesis, the reproductive toxicity of Dolichandrone serrulata flowers (DSFs) is not documented. Recent study aimed to evaluate the sub‐chronic toxicity of DSF on male reproductive system. Antioxidant capacity and total phenolic contents of DSF extract were determined using Folin–Ciocalteu's, 2,2‐diphenyl‐1‐picrylhydrazyl and ferric reducing antioxidant power assays. The terpenoid components were determined using nuclear magnetic resonance spectrum. Adult male rats were treated orally with DSF (100, 300 or 600 mg/kg) for 48 days. Histopathology of testis and epididymis was observed. Sperm concentration, viability, acrosome status and morphology were also examined. Expressions of heat shock protein 70 (Hsp70), tyrosine‐phosphorylated (TyrPho) proteins, androgen receptor (AR) and steroidogenic acute regulatory (StAR) protein in testis were investigated. Results showed that DSF contained phenolic compounds and terpenoids (phytoandrogens; rengyolone and cleroindicin B). No reproductive histopathology was observed in DSF‐treated rats. Although DSF decreased the serum testosterone level, the sperm qualities were not affected. Particularly, sperm concentration of DSF‐treated animals was significantly increased. DSF changed the testicular TyrPho proteins but the expression of AR, StAR or Hsp70 was not altered. In conclusion, DSF possesses antioxidant capacity with no toxicity on male reproductive system.

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Section: Discussionsupporting

confidence: 92%

The effect of Dolichandrone serrulata (wall. ex DC.) Seem. flower extract containing antioxidant capacity and terpenoids on the male reproductive system

et al. 2021

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“…The seed extract had been previously demonstrated to have antioxidant capacity and total phenolic compounds with no toxicity on male reproductive system (Iamsaard et al. 2020 ). Recently, this TMP extract has been already proven by our research group to contain l -DOPA determined by NMR and HPLC analyses (Choowong-In et al.…”

Section: Methodsmentioning

confidence: 99%

“…The selected doses used in this study were based on its property to increase the testosterone level, sperm quality and essential testicular proteins as previously demonstrated (Iamsaard et al. 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, TMP seed has been shown to possess high antioxidant capacity and to increase the expressions of testicular proteins (AR, AKAP4 [A-kinase anchor protein 4] and TyrPho proteins), testosterone level and sperm concentration in healthy rats (Iamsaard et al. 2020 ). Since TMP seeds seemed to enrich male reproductive system and its protective effect in stress animal models has never been reported, this study investigates improvement of the seed extract on reproductive damage in male rats induced by CS.…”

Section: Introductionmentioning

confidence: 99%

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Seed extract of Thai Mucuna pruriens reduced male reproductive damage in rats induced by chronic stress

Lapyuneyong

Tangsrisakda

Choowong-In

et al. 2022

Pharmaceutical Biology

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Context Thai Mucuna pruriens (L.) DC. var. pruriens (Fabaceae) (TMP) is known to enrich reproduction but preventive effects on stress related adverse reproductive parameters are not documented. Objective This study investigates the protective property of TMP seed extract on reproductive damage under chronic stress (CS). Materials and methods Male Sprague-Dawley rats were divided into four groups. The control and CS groups received distilled water, whereas the pre-treated rats received the aqueous TMP seed extract at doses of 150 and 300 mg/kg BW for 20 days before co-treatments with CS induction (immobilization and forced swimming) for 81 days. Serum was used to determine the cortisol and testosterone levels. Histology of testis and epididymis was observed with localization of androgen receptor (AR). Sperm parameters and the expression of steroidogenic acute regulatory (StAR), cytochrome P450 family 11 subfamily a member 1 (CYP11A1), AR, HSP70, caspases (3 and 9) and tyrosine phosphorylation (TyrPho) proteins were investigated. Results TMP extract improved cortisol level (0.84 ± 0.02 µg/dL) and protected against the damage of reproductive tissues and sperm parameters (count [49.78 ± 3.74 million sperm/mL], viability [90.01 ± 1.17%] and precocious acrosome reaction [1.38 ± 0.48%]). Expression of testicular StAR, CYP11A1, AR and HSP70 proteins was improved. Caspase expression was decreased in treated rats. TMP increased AR expression in CS sperm. Moreover, TyrPho protein expression was corrected after TMP administration. Conclusions TMP seed protected against adverse reproductive parameters in CS via improvements of functionally testicular markers and reductions of apoptotic proteins. It is possible to develop the TMP beans as an alternative medicine in treating of male subfertility caused by CS.

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“…In the process of functional maturation, it is possible that AKAP4 is secreted from the epididymis to further facilitate the initial motility on sperm membrane before capacitation in the female reproductive tract. Previously, its expression was shown to be decreased in germ cells of infertile males with high oxidative stress (Malcher et al., 2013; Nixon et al., 2019) but sperm AKAP4 is increased and associated with changes of TyrPho proteins after administration with antioxidant extract (Iamsaard et al., 2020). Indeed, the androgen production requires tyrosine phosphorylation in the MAPK proteins such as ERK1/2 of the Leydig cell after LH triggering (Gómez et al., 2013).…”

Section: Introductionmentioning

confidence: 99%

Decreased expression of AKAP4 and TyrPho proteins in testis, epididymis, and spermatozoa with low sexual performance of mice induced by modified CUMS

et al. 2021

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The molecular mechanism of chronic stress especially reduced motility, a major cause of male infertility, has not been proved. It is known that A‐kinase anchor protein 4 (AKAP4) and tyrosine‐phosphorylated (TyrPho) proteins are involved in progressive motility. This study aimed to investigate the effect of chronic unpredictable mild stress (CUMS) on sexual behaviours, sperm quality, and expressions of AKAP4 and TyrPho proteins in testis, epididymis, and spermatozoa. Sixteen male mice were divided into control and CUMS groups (n = 8/group). Animals were induced by a stressor from twelve stressors for 36 days. Sexual behaviours, corticosterone and testosterone, sperm parameters, and histopathology were observed. The expressions of AKAP4 and TyrPho proteins in testis, epididymis, and spermatozoa were examined. Results showed that CUMS significantly increased corticosterone while serum testosterone level was decreased. Sexual behaviours and sperm parameter quality were significantly decreased. CUMS mice showed vacuolisation and pyknotic cells in seminiferous epithelium and less sperm mass was observed within epididymal lumen. CUMS decreased expressions of AKAP4 and TyrPho proteins in testis, epididymis, and spermatozoa. In conclusion, the decreased expression of AKAP4 and TyrPho proteins may be a mechanism associated with low semen qualities particularly decrease of sperm motility in CUMS.

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Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds

Cited by 18 publications

References 34 publications

The effect of Dolichandrone serrulata (wall. ex DC.) Seem. flower extract containing antioxidant capacity and terpenoids on the male reproductive system

The effect of Dolichandrone serrulata (wall. ex DC.) Seem. flower extract containing antioxidant capacity and terpenoids on the male reproductive system

Seed extract of Thai Mucuna pruriens reduced male reproductive damage in rats induced by chronic stress

Decreased expression of AKAP4 and TyrPho proteins in testis, epididymis, and spermatozoa with low sexual performance of mice induced by modified CUMS

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