Evaluation of Antioxidant Efficiency of Croton tiglium L. Seeds Extracts after Incorporating Silver Nanoparticles

Abstract

T HIS STUDY aimed to enhance efficiency of the different Croton tiglium (C. tiglium) seeds extracts by incorporation of silver nanoparticles (Ag-NPs) through raised up cytotoxicity against growth of human colon cancer cells. C. tiglium seeds contain various phytoconstituents e.g., carbohydrates (glycosides), flavonoids, sterols (triterpenes), alkaloids and proteins. The protein was hydrolyzed in the dried aqueous seeds extract into free amino acids, the essential amino acids (20.71%) & nonessential amino acids (79.29%). Furthermore, the mucilage in the dried aqueous seeds extract and the lipoidal contents in the saponifiable matter of the petroleum ether extract were represented by total identified sugars represent ~81.21 % of the total mucilage hydrolyzate. The lipoidal contents in the saponifiable matter of the petroleum ether extract were represented by total saturated fatty acids (13.68%), total monoenoic fatty acids (34.49%), total dienoic fatty acids (45.76%) and total identified fatty acids (93.93%). Moreover, the unsaponifiable matter was represented by saturated hydrocarbons (40.73%), unsaturated hydrocarbons (18.93%), fatty alcohols (10.08%), sterols (10.61%) and total identified compounds (80.35%). In addition, there were four compounds (β sitosterol, α-amyrin, Oleanolic acid and 3-O-β-D-glucopyranosyl-β-sitosterol (daucosterol)) isolated from the petroleum ether extract. Additionally, incorporation of Ag-NPs into the extract caused no toxicity on the experimental animals when administrated orally. It was found that the median lethal dose (LD 50) of the ethanolic, petroleum ether and aqueous seeds extract-Ag nanocomposites was about 7.95, 5.2 and 65 ml/Kg, respectively.

Ameliorative and protective activity of Silver Nanoparticles (AgNPs) Biosynthesized Using Taraxacum officinale L. leaves Extract against 1, 2-dimethylhydrazine (DMH) Induced Colon Cancer in Rats

“…TOL-AgNPs normalized these in all treated phases (preservation & treatment). Those could be explained by the existence of a diverse variety of polyphenolics which were elevated by the incorporation of AgNP, as proposed by (Abdelhady and Badr, 2016) and protected by (Mahmoud Aboulthana et al, 2019) who discovered TOL-AgNPs hold excessive amounts of bioactive substances with antioxidant capacity in comparison to the leaf extracts. As a consequence, these compounds demonstrated a strong capacity to protect membrane permeability toward ROS produced by DMH injection.…”

Neuroprotective and Anti—Neuroinflammatory Effects of a Poisonous Plant Croton tiglium Linn. Extract

“…Therefore, the ethanolic CTE has been used as a topical application, shampoo, or soap [14]. More recently, the antioxidant effect of CTE has been evaluated, and the efficiency of the extract was found to be enhanced after the incorporation of nanoparticles [16]. Antioxidant, pain relief, and anti-inflammatory properties are important features required in the treatment and prevention of many neurological diseases related to neuroinflammation.…”