Evaluation of Antiradical and Anti-Inflammatory Activities of Ethyl Acetate and Butanolic Subfractions ofAgelanthus dodoneifolius(DC.) Polhill & Wiens (Loranthaceae) Using Equine Myeloperoxidase and Both PMA-Activated Neutrophils and HL-60 Cells

Abstract

The ethyl acetate and n-butanolic subfractions of Agelanthus dodoneifolius were investigated for their antioxidant and antimyeloperoxidase (MPO) activities. The reactive oxygen species (ROS) generation was assessed by lucigenin-enhanced chemiluminescence (CL) and dichlorofluorescein- (DCF-) induced fluorescence techniques from phorbol myristate acetate- (PMA-) stimulated equine neutrophils and human myeloid cell line HL-60, respectively. In parallel, the effects of the tested subfractions were evaluated on the total MPO release by stimulated neutrophils and on the specific MPO activity by means of immunological assays. The results showed the potent activity of the butanolic subfraction, at least in respect of the chemiluminescence test (IC50 = 0.3 ± 0.1 µg/mL) and the ELISA and SIEFED assays (IC50 = 2.8 ± 1.2 µg/mL and 1.3 ± 1.0 µg/mL), respectively. However, the ethyl acetate subfraction was found to be the most potent in the DCF assay as at the highest concentration, DCF fluorescence intensity decreases of about 50%. Moreover, we demonstrated that the ethyl acetate subfraction was rich in catechin (16.51%) while it was not easy to identify the main compounds in the butanolic subfraction using the UPLC-MS/MS technique. Nevertheless, taken together, our results provide evidence that Agelanthus dodoneifolius subfractions may represent potential sources of natural antioxidants and of antimyeloperoxidase compounds.

Gallic Acid Content in Taiwanese Teas at Different Degrees of Fermentation and Its Antioxidant Activity by Inhibiting PKCδ Activation: In Vitro and in Silico Studies

“…Gallic acid could reduce ROS levels in SH-SY5Y cells induced by 6-hydrodopamine autoxidation [22]. In addition, gallic acid was tested for ROS production in neutrophils, a main producer of superoxide, in which IC 50 value of 0.40 µg/mL was reported for ROS inhibition in PMA-treated neutrophils cells [23], and ROS significantly reduced by gallic acid were found at concentrations of 50–100 μM in LPS-induced cells [24]. Therefore, our study suggested that gallic acid at low concentrations (0 and 0.50 µM) similarly behaved as ROS stimulators, which occurred with cancer cells, but at high concentration (1.00 µM) gallic acid changed its function to that of an antioxidant.…”

Unlocking the Real Potential of Black Soldier Fly (Hermetia illucens) Larvae Protein Derivatives in Pet Diets

“…Human myeloid HL-60 cell line was purchased from American Type Culture Collection (Manassas, USA) and cultured according to the method described by Boly et al [ 32 ]. At the beginning of each assay, (1) the cell count of the suspension was estimated to maintain cellular density of 10 6 cells/mL and (2) cell viability was measured using trypan blue assay to main viability > 95%.…”

Agelathus dodoneifolius (African Mistletoe): A Review

“…A. dodoneifolius dried and powdered leaves exhibited a significant dose dependent radical scavenging activity with EC50 values of 8.20 ± 0.25 and 13.20 ± 0.37 µg/mL, respectively. It has also been reported that A. dodoneifolius subfractions represent potential sources of natural antioxidants and they are antimyeloperoxidase compounds [22].…”