Evaluation of Antiseptic Antiviral Activity of Chemical Agents

Geller, Chloé; Finance, C.; Duval, Raphaël E.

doi:10.1002/0471143030.cb2610s51

Cited by 2 publications

(3 citation statements)

References 5 publications

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“…For partial purification of the pseudoviruses to remove Triton X-100, the gel filtration chromatography was used [18]. Briefly, Sephadex G-25 (30 g/L) with a ratio of 1:1 (v/v) Sephadex/PBS was poured in combed cotton-stuffed syringe.…”

Section: Pseudovirus Extraction and Purificationmentioning

confidence: 99%

“…One group, containing low-risk HPVs, is responsible for benign genital warts and mainly includes types 6,11,42,43. The other group, consisting of high risk HPVs, mostly includes types 16,18,31,33,35,39,45,51,52,56,59, 66, 68 and 70 which lead to cancer in the anogenital and oropharyngeal areas [2,3]. HPV types 16, 18, 31 and 45 account for more than 90% of cervical cancers, among them genotype 16 is found in almost more than half of the cervical cancers worldwide [3,5].…”

Section: Introductionmentioning

confidence: 99%

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Human papillomavirus genotype 16 pseudovirus production and purification in HEK-293FT cells

Barzegar

Sharifi

Langroudi

et al. 2017

vacres

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Introduction: Human papillomavirus (HPV) is the main causative agent of cervical cancer worldwide leading to a big health problem, especially in the developing countries. Among 14 common high-risk genotypes, HPV16 accounts for more than 50% of all cervical cancers. The current prophylactic vaccines against HPV infection are based on L1 protein. Due to some drawbacks in the current vaccines such as their restrictions to genotype and relatively high costs, second-generation HPV vaccines, based on L2 protein, are under development. The evaluation of the neutralization efficiency of the antibodies against the virus needs pseudoviruses consisting of L1 and L2 capsid proteins harboring a reporter gene. The aim of this study was assembling HPV16 L1 and L2 proteins into a pseudovirion that contains a reporter plasmid. Methods: HPV16 L1 and L2 coding plasmid along with pEGFP-N1 were amplified in E. coli host and were extracted before transfection with DNA-calcium phosphate deposition method into the HEK 293FT cells. The expression of HPV16 pseudoviruses was confirmed by fluorescence microscopy and flow cytometry. Pseudoviruses were partially purified with gel chromatography and then were visualized by atomic force microscopy. Results: Simultaneous transfection of HPV16 L1 and L2 coding plasmid along with pEGFP-N1 into the HEK 293FT cells resulted in self-assembly of 45-55 nm pseudoviruses, harboring the reporter gene coding plasmid. Conclusion: In this project, HPV16 pseudoviruses, harboring a reporter gene were produced and partially purified, and their infecting capability was assessed. The pseudovirions could be used for neutralization assay and also as vectors for gene therapy.

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Section: Pseudovirus Extraction and Purificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Human papillomavirus genotype 16 pseudovirus production and purification in HEK-293FT cells

Barzegar

Sharifi

Langroudi

et al. 2017

vacres

View full text Add to dashboard Cite

show abstract

“…However, few efficient and specific measures are available to oppose most of these infections, especially when the etiological agent is viral 6 . Many chemical agents have been used for topical use in dentistry to reduce the transmission of microorganisms, including povidone-iodine (PVP-I) and chlorhexidine, which have moderate and weak action, respectively, concerning viruses 7,8 .…”

Section: Introductionmentioning

confidence: 99%

Do Oral Antiseptics Used in the Dental Routine Have Anti-Viral Efficacy? A Review Systematic in Vitro Studies

Viana,

Freire,

Simão

et al. 2024

REV. CIÊNC. PLURAL

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Introdução : Os enxaguantes bucais desempenham um papel importante na clínica odontológica, mas seu papel sobre os vírus requer investigação. Objetivo : revisar estudos in vitro para identificar o efeito de diferentes enxaguantes bucais sobre os principais vírus associados ao atendimento odontológico de rotina. Metodologia :As seguintes bases de dados foram pesquisadas até dezembro de 2022: bases de dados PubMed, Embase, Scopus e Web of Science; a Biblioteca Cochrane e a Biblioteca Virtual em Saúde (BVS); e literatura cinzenta. Foram selecionados estudos in vitro que utilizaram enxaguantes bucais com o objetivo de reduzir a carga viral. A questão focal foi proposta seguindo o princípio PICOS: a População (vírus envolvidos na etiologia da infecção oral), a Intervenção (antissépticos orais), o comparador apropriado (controles positivos e negativos) e, os Desfechos de interesse (redução da carga viral ) e o desenho do estudo (estudos in vitro). Resultados :Considerando os critérios de elegibilidade, foram incluídos 19 artigos para esta revisão. A eficácia de iodopovidona (PVP-I), clorexidina, Listerine, óleos essenciais e lavagens com cloreto de cetilpiridínio foi investigada. O PVP-I teve seus efeitos principalmente associados ao SARS-CoV, demonstrando redução significativa da carga viral após 15 segundos de exposição. A clorexidina foi considerada ineficaz contra os vírus respiratórios adenovírus, poliovírus e rinovírus. Listerine demonstrou eficácia superior contra os vírus HSV-1 e 2 e vírus influenza A, e o cloreto de cetilpiridina também demonstrou atividade virucida contra influenza A. Conclusões :O tipo, concentração e tempo de exposição aos antissépticos variaram entre os estudos. O PVP-I e o digluconato de clorexidina foram as substâncias mais estudadas, mas no geral o PVP-I foi mais eficaz na redução dos títulos virais, principalmente no que diz respeito aos coronavírus. Outros antissépticos como CPC, H2O2 e Listerine® também demonstraram redução significativa da carga viral, mas este é um número limitado de estudos. Palavras-chave: enxaguatórios bucais; vírus; efeito.

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Evaluation of Antiseptic Antiviral Activity of Chemical Agents

Cited by 2 publications

References 5 publications

Human papillomavirus genotype 16 pseudovirus production and purification in HEK-293FT cells

Human papillomavirus genotype 16 pseudovirus production and purification in HEK-293FT cells

Do Oral Antiseptics Used in the Dental Routine Have Anti-Viral Efficacy? A Review Systematic in Vitro Studies

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