Evaluation of Antitumor Potential of Forskolin and Andrographolide Employing Potato Tumor Bioassay Model

Suresh, Velpula; Raju, D.; Rupula, Karuna; Godugu, Deepika; B, Sashidhar R.

doi:10.2174/2212697x03666160524161911

Cited by 5 publications

(5 citation statements)

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“…20) . A similar antibacterial activity experiment was performed by Suresh et al [17] against A. tumefaciens with the labdane diterpene molecules forskolin and andrographolide and observed that the compounds did not inhibit the growth of A. tumefaciens .…”

Section: Discussionsupporting

confidence: 57%

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Synthesis, characterisation and anti‐tumour activity of biopolymer based platinum nanoparticles and 5‐fluorouracil loaded platinum nanoparticles

Godugu

Beedu

2018

IET nanobiotechnol.

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A facile and green synthesis of platinum nanoparticles [gum kondagogu platinum nanoparticles (GKPtNP)] using biopolymer-gum kondagogu was developed. The formation of GKPtNP was confirmed by ultraviolet (UV)-visible spectroscopy, scanning electron microscopy-energy dispersive X-ray spectroscopy, transmission electron microscopy, X-ray diffraction, Zeta potential, Fourier transform infrared, inductively coupled plasma mass spectroscopy. The formed GKPtNP are well dispersed, homogeneous with a size of 2-4 ± 0.50 nm, having a negative zeta potential (−46.1 mV) indicating good stability. 5-Fluorouracil (5FU) was loaded onto the synthesised GKPtNP, which leads to the development of a new combination of nanomedicine (5FU-GKPtNP). The in vitro drug release studies of 5FU-GKPtNP in pH 7.4 showed a sustained release profile over a period of 120 min. Agrobacterium tumefaciens induced in vitro potato tumour bioassay was employed for screening the anti-tumour potentials of GKPtNP, 5FU, and 5FU-GKPtNP. The experimental results suggested a complete tumour inhibition by 5FU-GKPtNP at a lower concentration than the GKPtNP and 5FU. Furthermore, the mechanism of anti-tumour activity was assessed by their interactions with DNA using agarose gel electrophoresis and UV-spectroscopic analysis. The electrophoresis results revealed that the 5FU-GKPtNP totally diminishes DNA and the UV-spectroscopic analysis showed a hyperchromic effect with red shift indicating intercalation type of binding with DNA. Over all, the present study revealed that the combined exposure of the nanoformulation resulted in the enhanced anti-tumour effect.

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Section: Discussionsupporting

confidence: 57%

“…The number of tumours formed on each disc was counted using an inverted microscope (Olympus, India). The total sugars and protein content in potato tumour discs were estimated as described by Suresh [17].…”

Section: Methodsmentioning

confidence: 99%

Synthesis, characterisation and anti‐tumour activity of biopolymer based platinum nanoparticles and 5‐fluorouracil loaded platinum nanoparticles

Godugu

Beedu

2018

IET nanobiotechnol.

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“…The present experimental results validate the antioxidant and anticancer properties of the synthesised GKCPt NC. The mechanism of antitumour activity could be inhibition of topoisomerase, interference with tubulin function or prevention of microtubule reorganisation and the results are comparable with mammalian cell lines [59, 70]. Godugu and Sashidhar [74] reported antitumour activity of GKPt NP through potato tumour assay and the IC 50 was found to be 10.1 μg ml −1 .…”

Section: Resultsmentioning

confidence: 88%

“…Assay performed in two steps and was executed as per the method reported by Suresh et al [70]. The first step comprises, evaluation of the antibacterial activity of GK, GKCPt NC, carboplatin while Pen‐Strep was used as a positive control.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and characterisation of novel biopolymer stabilised organic Pt‐nanocomposite: assessment of its antioxidant and antitumour properties

Katukam

Rupula

Beedu

2020

IET nanobiotechnol.

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“…UV visible absorption experiments are often used to monitor the binding mode of interaction between the small molecule and macromolecule, DNA, or proteins. UV spectroscopic study of andrographolide with CT DNA revealed a hypochromic effect: a decrease in absorbance, and a blue shift: a slight shift of band towards the shorter wavelength when interacted with CT DNA [115].…”

Section: Interaction Studiesmentioning

confidence: 99%

An insight into the pharmacological and analytical potential of Andrographolide

Agrawal

Nair

2022

Fundamemntal Clinical Pharma

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Andrographis paniculata is an annual medicinal herb from the family Acanthaceae. Andrographolide is generally considered an essential bioactive component of plant A. paniculata. Since ancient times, it has been widely recognized for its therapeutic qualities and has attracted the scientific and medical communities' attention. This review summarizes the molecular, clinical, and in vitro research of compound andrographolide and its mechanism of action. Andrographolide, when combined with other enhancing agents, offers a wide variety of health benefits. The therapeutic potential of andrographolide has been exemplified and exhibited by directly regulating genes and indirectly interacting with small molecules and different enzymes. This review compiles and consolidates the pharmacological action of andrographolide and its analogs and deciphers the gaps that have hindered its use in medicinal research.

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Evaluation of Antitumor Potential of Forskolin and Andrographolide Employing Potato Tumor Bioassay Model

Cited by 5 publications

References 0 publications

Synthesis, characterisation and anti‐tumour activity of biopolymer based platinum nanoparticles and 5‐fluorouracil loaded platinum nanoparticles

Synthesis, characterisation and anti‐tumour activity of biopolymer based platinum nanoparticles and 5‐fluorouracil loaded platinum nanoparticles

Synthesis and characterisation of novel biopolymer stabilised organic Pt‐nanocomposite: assessment of its antioxidant and antitumour properties

An insight into the pharmacological and analytical potential of Andrographolide

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