Evaluation of apoptotic effect of cyclic imide derivatives on murine B16F10 melanoma cells

Machado, Karina Elisa; Oliveira, Kely Navakoski de; Santos-Bubniak, Lorena; Licínio, Marley Aparecida; Nunes, Ricardo José; Santos-Silva, Maria Cláudia

doi:10.1016/j.bmc.2011.09.008

Cited by 22 publications

(14 citation statements)

References 35 publications

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“…Indeed, cells in early apoptosis still have their intact membranes therefore have the green core, but not uniformly stained, chromatin condensation occurring in them, cleavage of DNA and/or nuclear fragmentation, these are no longer stuck and its morphology was changed, since cells in late apoptosis show chromatin condensation and orange areas in the nucleus, because in the final stages of the process have lost membrane integrity and ethidium bromide on the predominant acridine orange. In the control group we can observe living cells with nuclei well formed and adhered to the blade [ 26 ]. The difference of activity when a large excess of the Hex and EA extracts was added to the assay system, could be explained by the inhibition of the penetration through the cell membrane at high doses of extract components [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma

Boubaker

Bzéouich

Nasr

et al. 2015

BMC Complement Altern Med

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BackgroundHere, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated.MethodsThe Apoptosis was evidenced by investigating DNA fragmentation, and Acridine orange/ethidium bromide staining. Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.ResultsExtracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation. Our result was confirmed by ladder DNA fragmentation profile. All extracts showed also the ability to enhance melanogenesis and tyrosinase activity of B16F10 melanoma cells.ConclusionThe tested extracts have a significant biological effect which may be due to their bioactive compounds.

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Section: Discussionmentioning

confidence: 99%

Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma

Boubaker

Bzéouich

Nasr

et al. 2015

BMC Complement Altern Med

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“…Cyclic imide derivatives exhibiting anti-inflammatory (Abdel-Aziz et al, 2011;Al-suwaidan et al, 2013), antibacterial (Kumari and Singh, 2014), antinociceptive (de oliveira et al, 2012), anticonvulsant (Sharma et al, 2008a, b), anticancer (Machado et al, 2011;El-Azab et al, 2013), hypolipidemic (El-Zahabi et al, 2012 and hypnotic (Byun et al, 2008) activities are well reported in literature. Cyclic imide derivatives are also inhibitors of amino peptidase N (Li et al, 2010) and Mycobacterium tuberculosis protein tyrosine phosphatase B .…”

Section: Introductionmentioning

confidence: 97%

Synthesis of acridine cyclic imide hybrid molecules and their evaluation for anticancer activity

et al. 2015

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9-Amino acridine derivatives (1a-e) on condensation with dihydrofuran-2,5-dione, hexahydroisobenzofuran-1,3-dione and isochroman-1,3-dione under microwave irradiation gave corresponding condensation products 2a-e, 3a-e and 4a-e, respectively, in good yields. All these compounds were screened in vitro for anticancer activity against five human cancer cell lines, i.e., breast (T47D), lung (NCl H-522), colon (HCT-15), ovary (PA-1) and liver (Hep G2). Compounds 1-(3-methoxyacridine-9-yl) pyrrolidine-2,5-dione (2d) (ovary PA-1), 2-(2-methylacridine-9-yl)hexahydro-1H-isoindole-1,3(2H)-dione (3b) (lung NCl H-522), 2-(4-methylacridine-9-yl)hexahydro-1H-isoindole-1,3(2H)-dione (3c) (ovary PA-1), 2-(4-methoxyacridine-9-yl)hexahydro-1H-isoindole-1,3(2H)-dione (3e) (ovary PA-1) and 2-(2-methylacridine-9-yl)isoquinoline-1,3(2H,4H)-dione (4b) (ovary PA-1) exhibited IC 50 values 7.1, 8.0, 5.4, 10.0 and 6.56 lM, respectively, and hence possess good anticancer activity.

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“…Some estrone derivatives with the D-ring replaced with the glutarimide moiety showed potent inhibition of steroid sulfatase, an enzyme involved in the pathway of the development of hormone-dependent breast tumors 23 ; while aminoglutethimide, the non-steroidal aromatase inhibitor, is in use for the treatment of hormone-sensitive metastatic breast cancer 24,25 . In the past decade, antitumor activity in vitro of mitonafide 26 , amonafide 27 and naphthalimide 28,29 derivatives was intensively examined. Upon detail analysis of bioactive compounds that comprise glutarimide moiety in their structure, as is briefly outlined in previous paragraphs, we concluded that data on antiproliferative and on antibacterial activity of compounds 1-9 ( Figure 1) cannot be found in literature.…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative and antibacterial activity of some glutarimide derivatives

Popović‐Djordjević¹,

Klaus

Žižak

et al. 2015

Journal of Enzyme Inhibition and Medicinal Chemistry

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Antiproliferative and antibacterial activities of nine glutarimide derivatives (1-9) were reported. Cytotoxicity of compounds was tested toward three human cancer cell lines, HeLa, K562 and MDA-MB-453 by MTT assay. Compound 7 (2-benzyl-2-azaspiro[5.11]heptadecane-1,3,7-trione), containing 12-membered ketone ring, was found to be the most potent toward all tested cell lines (IC 50 ¼ 9-27 mM). Preliminary screening of antibacterial activity by a disk diffusion method showed that Gram-positive bacteria were more susceptible to the tested compounds than Gram-negative bacteria. Minimum inhibitory concentration (MIC) determined by a broth microdilution method confirmed that compounds 1, 2, 4, 6-8 and 9 inhibited the growth of all tested Gram-positive and some of the Gram-negative bacteria. The best antibacterial potential was achieved with compound 9 (ethyl 4-(1-benzyl-2,6-dioxopiperidin-3-yl)butanoate) against Bacillus cereus (MIC 0.625 mg/mL; 1.97 Â 10 À3 mol/L). Distinction between more and less active/ inactive compounds was assessed from the pharmacophoric patterns obtained by molecular interaction fields.

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Evaluation of apoptotic effect of cyclic imide derivatives on murine B16F10 melanoma cells

Cited by 22 publications

References 35 publications

Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma

Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma

Synthesis of acridine cyclic imide hybrid molecules and their evaluation for anticancer activity

Antiproliferative and antibacterial activity of some glutarimide derivatives

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