Evaluation of Apoptotic Signals in Human Cancer Malignant Melanoma Me45 and Ovarian Carcinoma SKOV3 Cell Death after Chemotherapeutic Reaction with Cisplatin

Abstract

Abstract：Objective: The aim of this study was to evaluate the influence of chemotherapy with cisplatin (CTcisplatin) on cell death induction in human metastatic and resistant cancer cells. Methods: There were investigated the effects of chemotherapy with cisplatin (10µM). Two lines were used: human metastatic melanoma (Me45) and human ovarian carcinoma cell line resistance to cisplatin (SKOV-3). The cytotoxic effect of cisplatin by viability assay, trypan blue, apoptotic cell death by TUNEL assay and the expression of caspase 3 and 7 by immunocytochemistry were examined. Results: Cisplatin induced decreased cellular proliferation proportionally with increasing concentration and longer incubation time. The cytotoxic effect was more significant in melanoma cells. The necrosis was observed in 3% of ovarian carcinoma cells and in 8% of malignant melanoma cells, while apoptosis was observed in 1% of ovarian carcinoma cells and in 39% of malignant melanoma cells in comparison to control untreated cells. The increased expression of caspase 3 and 7 was detected after CT-cisplatin. Conclusion: The dose of cisplatin applied in the studies was sufficient to treatment of malignant melanoma. Our studies confirmed the resistance of human ovarian carcinoma to cisplatin.