Evaluation of Asymptomatic Malaria Status in Eastern of Afghanistan Using High Resolution Melting Analysis

Mosawi, Sayed Hussain; Dalimi, Abdolhossein; Safi, Najibullah; Ghaffarifar, Fatemeh; Sadraei, Javid

doi:10.18502/ijpa.v15i2.3299

Cited by 8 publications

(9 citation statements)

References 29 publications

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“…Besides, melting curve alteration in HRM method discriminated Iranian Leishmania parasites of L. major , L. tropica and mixed infection in the study of Ghafari SM et al 34 . In addition, Mosawi SH et al, on asymptomatic malaria status in eastern Afghanistan, could distinguish P. vivax , P. falciparum , and mixed infections using high-resolution melting analysis 35 . The differentiation of Mitragyna speciosa from allied Mitragyna species was performed using DNA barcoding‑high‑resolution melting analysis by Chayapol Tungphatthong et al 36 .…”

Section: Discussionmentioning

confidence: 99%

Discrimination of human papillomavirus genotypes using innovative technique nested-high resolution melting

Alirezaei

Mosawi²,

Afgar

et al. 2022

Sci Rep

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The prompt detection of human papillomavirus and discrimination of its genotypes by combining conventional methods in new molecular laboratories is essential to achieve the global call of eliminating cervical cancer. After predicting the melting temperature of an approximately 221 bp region of the L1 gene from different HPV genotypes by bioinformatics software, an innovative technique based on the nested- high resolution melting was designed with three approaches and using conventional PCR, qPCR, and diagnostic standards. HPV-positive samples identified by microarray along with diagnostic standards were evaluated by qPCR-HRM and discordant results were subjected to sequencing and analyzed in silico using reference types. In addition to screening for human papillomavirus, nested-qPCR-HRM is one of the modified HRM techniques which can discriminate some genotypes, including 6, 16, 18, 52, 59, 68 and 89. Despite the differences in diagnostic capabilities among HRM, microarray and sequencing, a number of similarities between HRM, and sequencing were diagnostically identified as the gold standard method. However, the bioinformatics analysis and melting temperature studies of the selected region in different HPV genotypes showed that it could be predicted. With numerous HPV genotypes and significant genetic diversity among them, determining the virus genotype is important. Therefore, our goal in this design was to use the specific molecular techniques with several specific primers to increase sensitivity and specificity for discriminating a wide range of HPV genotypes. This approach led to new findings to evaluate the ability of different approaches and procedures in accordance with bioinformatics.

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Section: Discussionmentioning

confidence: 99%

Discrimination of human papillomavirus genotypes using innovative technique nested-high resolution melting

Alirezaei

Mosawi²,

Afgar

et al. 2022

Sci Rep

Self Cite

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“…Malaria is a common endemic disease in Afghanistan threatening about half of the Afghan population. Incidence has increased in recent years, especially in the Eastern regions [ 42 ]. In recent decades, the numbers of malaria cases has sharply increased in Afghanistan and—as with leishmaniasis—many outbreaks of the disease have been recorded [ 43 ].…”

Section: Infectious Diseasesmentioning

confidence: 99%

Health Status of Afghan Refugees in Europe: Policy and Practice Implications for an Optimised Healthcare

Matsangos¹,

Ziaka

Exadaktylos

et al. 2022

IJERPH

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Four decades of civil war, violence, and destabilisation have forced millions of Afghans to flee their homes and to move to other countries worldwide. This increasing phenomenon may challenge physicians unfamiliar with the health status of this population, which may be markedly different from that of the host country. Moreover, several factors during their migration, such as transport in closed containers, accidental injuries, malnutrition, and accommodation in detention centres and refugee camps have a major influence on the health of refugees. By taking into account the variety of the specific diseases among migrant groups, the diversity of the origins of refugees and asylum seekers, and the increasing numbers of Afghan refugees, in this review we focus on the population of Afghans and describe their health status with the aim of optimising our medical approach and management. Our literature review shows that the most prevalent reported infections are tuberculosis and other respiratory tract infections and parasitic diseases, for example leishmaniasis, malaria, and intestinal parasitic infections. Anaemia, hyperlipidaemia, arterial hypertension, diabetes, smoking, overweight, malnutrition, low socioeconomic status, and poor access to healthcare facilities are additional risk factors for non-communicable diseases among Afghan refugees. With regards mental health issues, depression and post-traumatic stress disorder (PTSD) are the most common diagnoses and culture shock and the feeling of being uprooted modulate their persistence. Further research is needed in order to provide us with extensive, high-quality data about the health status of Afghan refugees. The main objective of this review is to identify protective factors which could ensure key health concepts and good clinical practice.

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“…Afghanistan is another highly malaria-endemic country, where approximately half of the population is at risk of infection [ 78 ]. The incidence of malaria in Afghanistan exceeded 200,000 cases in 2013, with 1783 deaths [ 7 ].…”

Section: Epidemiological Data From Countries Of Origin For Importementioning

confidence: 99%

“…The latest WHO report projected nearly 400,000 malaria cases in Afghanistan in 2019 [ 4 ]. A study of malaria prevalence in Jalalabad City, employing real-time PCR with high resolution melting analysis on 296 blood samples reported asymptomatic malaria in 26 (7.8%) individuals [ 78 ]. Most of the imported malaria cases in Iran in one study originated from Afghanistan [ 62 ].…”

Section: Epidemiological Data From Countries Of Origin For Importementioning

confidence: 99%

Current Status and the Epidemiology of Malaria in the Middle East Region and Beyond

2021

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Vector-borne parasitic infectious diseases are important causes of morbidity and mortality globally. Malaria is one of the most common vector-borne parasitic infection and is caused by five Plasmodium species, namely P. falciparum, P. vivax, P. ovale, P. malariae, and P. knowlesi. Epidemiologically, differences in the patterns of malaria cases, causative agent, disease severity, antimicrobial resistance, and mortality exist across diverse geographical regions. The world witnessed 229 million malaria cases which resulted in 409,000 deaths in 2019 alone. Although malaria cases are reported from 87 countries globally, Africa bears the brunt of these infections and deaths as nearly 94% of total malaria cases and deaths occur in this continent, particularly in sub-Saharan Africa. Most of the Middle East Region countries are malaria-free as no indigenous cases of infection have been described in recent years. However, imported cases of malaria continue to occur as some of these countries. Indeed, the six Gulf Cooperation Council (GCC) countries have large expatriate population originating from malaria endemic countries. In this review, the current status and epidemiology of malaria in the Middle East Region countries and other malaria-endemic countries that are home to a large migrant workforce being employed in Middle East Region countries are discussed.

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Evaluation of Asymptomatic Malaria Status in Eastern of Afghanistan Using High Resolution Melting Analysis

Cited by 8 publications

References 29 publications

Discrimination of human papillomavirus genotypes using innovative technique nested-high resolution melting

Discrimination of human papillomavirus genotypes using innovative technique nested-high resolution melting

Health Status of Afghan Refugees in Europe: Policy and Practice Implications for an Optimised Healthcare

Current Status and the Epidemiology of Malaria in the Middle East Region and Beyond

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