Evaluation of autologous bone marrow‐derived nucleated cells for healing of full‐thickness skin wounds in rabbits

Abstract

The aim of the study was to evaluate the potential of autologous bone marrow-derived nucleated cells to enhance the rate of healing of full-thickness excisional skin wounds in rabbits. The study was conducted on 20 New Zealand white rabbits of either sex. Two, 2 x 2 cm full-thickness skin (thoracolumabar region) excisional wounds were created; one on each side of the dorsal midline in each animal. The wounds were randomly assigned to either injection of autologous bone marrow-derived nucleated cells into the wound margins (BI), or topical application of sterile saline solution (normal saline, NS), which served as control. The wound healing was assessed by evaluation of granulation tissue formation, wound contraction, epithelisation and histopathological and histochemical changes up to 28 days after creation of the wound. Granulation tissue appeared significantly faster in BI-treated wounds (3.22 +/- 0.22 days) than in NS-treated wounds (4.56 +/- 0.47 days). Better epithelisation was seen histologically in BI wounds than in NS-treated wounds. Wound contraction was significantly more in BI wounds when compared with NS wounds on 21 post-surgery. Histopathological examination of the healing tissue showed early disappearance of inflammatory reaction, significantly more neovascularisation, and more fibroplasias and early lay down and histological maturation of collagen in BI wounds than in control wounds. It was concluded that injection of autologous bone marrow-derived nucleated cells in the wound margins induced faster and better quality healing of excisional skin wounds in rabbits when compared with normal saline. The injection of autologous bone marrow-derived nucleated cells can be used to promote healing of large full-thickness skin wounds in rabbits.

Effect of Bone Marrow Derived Mesenchymal Stem Cells on Healing of Induced Full-Thickness Skin Wounds in Albino Rat

“…These findings might be attributed to the role of BMSCs in neovascularisation to sustain the newly formed granulation tissue (8,24,26). BMSCs could differentiate into endothelial cells and pericytes (27).…”

“…However, the injection of BMSCs was made slowly using a fine hypodermic needle to minimize the trauma. Furthermore, the injection induced trauma was considered negligible compared with the surgical trauma inflicted in creation of wound and thus may be of little significance (8).…”

“…Granulation tissue elevated above skin edges, projecting over the advancing border of epithelium. A granulation score of 3 was considered better than 4 or 2, which were considered better than 5 or 1 (8). Time of complete healing: Time of complete healing was recorded as the day on which wound healed completely.…”

“…Healing was considered complete when hairy skin covered the entire wound and the area of the remaining granulation tissue was zero. The duration required for complete wound healing was recorded in each animal and the mean duration needed to complete healing was calculated for all studied groups (8).…”

Equine Epidermis: A Source of Epithelial-Like Stem/Progenitor Cells with In Vitro and In Vivo Regenerative Capacities

“…Clinical applications of non-EpSCs for skin wound healing have been described [26,27]. In this regard, the improved healing capacity of persistent leg wounds in human diabetic patients in response to bone marrow-derived mesenchymal stem cell (MSC) treatment was reported [28].…”