Evaluation of automated loop-mediated amplification (LAMP) for routine malaria detection in blood samples of German travelers – A cross-sectional study

Frickmann, Hagen; Hinz, Rebecca; Rojak, Sandra; Bonow, Insa; Ruben, Stefanie; Wegner, Christine; Zielke, Iris; Hagen, Ralf Matthias; Tannich, Egbert

doi:10.1016/j.tmaid.2018.05.006

Cited by 29 publications

(45 citation statements)

References 35 publications

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“…None of these signals could be confirmed by any other applied diagnostic approach and no additional materials were available from these patients. However, the more extensive molecular testing in this study slightly decreased the calculated sensitivity to 96.4% (239/248) in comparison with 98.7% from the previous assessment [5]. The missed samples were from patients positive for P. falciparum (n = 7) who had already become negative by microscopy under therapy as well as for P. ovale (n = 1) and P. vivax (n = 1) with only a few parasites within the whole thick blood film each.…”

Section: Brief Summarycontrasting

confidence: 80%

“…Focusing on genus-specific Meridian illumigene/ alethia Malaria LAMP, the assessment confirmed three presumably falsely positive signals as it had already been described previously [5], confirming a specificity of 99.6% (769/772). None of these signals could be confirmed by any other applied diagnostic approach and no additional materials were available from these patients.…”

Section: Brief Summarysupporting

confidence: 79%

“…At the Bernhard Nocht Institute for Tropical Medicine, the German National Reference Center for Tropical Infectious Diseases, a variety of NAT assays for malaria screening and differentiation has been evaluated recently using identical samples [5,6]. Here, a meta-analysis of the results is presented, allowing a direct comparison of the assays and particularly focusing on discordant results affecting sensitivity and specificity.…”

Section: Brief Summarymentioning

confidence: 99%

“…In previous studies, a collection of 1020 well-characterized nucleic acid extractions [5,6] from ethylenediaminetetraacetic acid (EDTA) blood taken from patients with clinical suspicion of malaria and extracted using the EZ1 DNA Blood 200 μL Kit (Qiagen, Hilden, Germany) had been completely assessed by four different NAT approaches in addition to microscopy of Giemsa-stained thick and thin blood films: -genus-specific loop-mediated isothermal amplification (LAMP) using the Meridian illumigene/ alethia Malaria platform (Meridian Bioscience Inc., Cincinnati, OH, USA) [5] -species-specific polymerase chain reaction (PCR) using the "FTD Malaria differentiation" real-time multiplex PCR kit (Fast Track Diagnostics [FTD], Sliema, Malta) for all samples, of which only the ones positively screened by RealStar Malaria PCR Kit 1.0 (altona Diagnostics, Hamburg, Germany) had been published so far [6] -genus-specific screening PCR using the RealStar Malaria PCR Kit 1.0 with subsequent discrimination using the RealStar Malaria S&T PCR Kit 1.0 (altona Diagnostics) in case of positive screening results [6] -species-specific in-house real-time PCR for all samples (unpublished data) using a previously described in-house SybrGreen real-time PCR assay [7], slightly modified exactly as described [8].…”

Section: Brief Summarymentioning

confidence: 99%

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Meta-analysis of the diagnostic performance characteristics of three commercial and one in-house nucleic acid amplification tests for malaria screening

Altangerel

Frickmann

2020

Journal of Laboratory Medicine

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Background A meta-analysis of previously performed evaluation studies of nucleic acid amplification testing (NAT) approaches for the screening for and differentiation of Plasmodium spp. using identical samples was performed to comparatively assess their suitability for the routine diagnostic setting. Methods Three commercial NATs for malaria (one loop-mediated isothermal amplification [LAMP] assay, two real-time polymerase chain reactions [PCRs]) and one in-house real-time PCR were comparatively assessed with a collection of 1020 well-characterized ethylenediaminetetraacetic acid (EDTA) blood samples from patients with suspected or confirmed malaria. Results Altogether 765 (75%) concordantly negative and 223 (21.9%) concordantly positive results of the four molecular tests were obtained, while discordant results were seen in 32 (3.1%) instances. For genus-specific assays, the observed sensitivity and specificity ranges were 96.4%–98.4% and 99.6%–99.9%, and for species-specific assays, 94.0%–97.6% and 99.6%–100%, respectively. Falsely negative molecular test results comprised microscopically negative samples, samples at the microscopic detection threshold and quantitatively less abundant species in mixed infections. Conclusions Excellent test characteristics of all assessed assays with only minor differences encourage molecular malaria screening with genus- and species-specific NAT with discrepancies only within the borderline range of their detection thresholds.

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Section: Brief Summarycontrasting

confidence: 80%

Section: Brief Summarysupporting

confidence: 79%

Section: Brief Summarymentioning

confidence: 99%

Section: Brief Summarymentioning

confidence: 99%

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Meta-analysis of the diagnostic performance characteristics of three commercial and one in-house nucleic acid amplification tests for malaria screening

Altangerel

Frickmann

2020

Journal of Laboratory Medicine

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“…Blood counts were performed the same day using the Sysmex XP-300 automated hematology analyzer (Sysmex, Kobe, Japan). For PCR analyses EDTA-anticoagulated blood was centrifuged and the pellet was Hamburg, Germany) as described before [10,11].…”

Section: Methodsmentioning

confidence: 99%

High prevalence of asymptomatic malaria infections in adults, Ashanti Region, Ghana, 2018

Heinemann

Phillips

Vinnemeier

et al. 2020

Preprint

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Background Ghana is among the high-burden countries for malaria infection and recently reported a notably increase in malaria cases. While asymptomatic parasitemia is increasingly recognized as a hurdle for malaria elimination, studies on asymptomatic malaria are scarce and usually focus on children and on non-falciparum species. The present study aims to assess the prevalence of asymptomatic Plasmodium falciparum and non-falciparum infections in Ghanaian adults in the Ashanti region during the high transmission season. Methods Asymptomatic adult residents from five villages in the Ashanti Region, Ghana, were screened for Plasmodium spp. by rapid diagnostic test (RDT) and polymerase chain reaction (PCR) during the rainy season. Samples tested positive were subtyped using species-specific real-time PCR. For all P. ovale infections additional sub-species identification was performed.Results Molecular prevalence of asymptomatic Plasmodium infection was 284/391 (73%); only 126 (32%) infections were detected by RDT. While 266 (68%) participants were infected with Plasmodium falciparum, 33 (8%) were infected with Plasmodium malariae and 34 (9%) with Plasmodium ovale. The sub-species Plasmodium ovale curtisi and P. ovale wallikeri were identified to similar proportions. Non-falciparum infections usually presented as mixed infections with Plasmodium falciparum.Conclusions Most adult residents in the Ghanaian forest zone are asymptomatic Plasmodium carriers. The high Plasmodium prevalence not detected by RDT in adults highlights that malaria eradication efforts must target all members of the population. Beneath Plasmodium falciparum, screening and treatment must also include infections with Plasmodium malariae, P. ovale curtisi and P. ovale wallikeri .

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On detection thresholds–a review on diagnostic approaches in the infectious disease laboratory and the interpretation of their results

Hahn¹,

Podbielski²,

Meyer

et al. 2020

Acta Tropica

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Evaluation of automated loop-mediated amplification (LAMP) for routine malaria detection in blood samples of German travelers – A cross-sectional study

Cited by 29 publications

References 35 publications

Meta-analysis of the diagnostic performance characteristics of three commercial and one in-house nucleic acid amplification tests for malaria screening

Meta-analysis of the diagnostic performance characteristics of three commercial and one in-house nucleic acid amplification tests for malaria screening

High prevalence of asymptomatic malaria infections in adults, Ashanti Region, Ghana, 2018

On detection thresholds–a review on diagnostic approaches in the infectious disease laboratory and the interpretation of their results

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