Evaluation of Avian-Specific Probiotic and Salmonella Enteritidis-, Salmonella Typhimurium-, and Salmonella Heidelberg-Specific Antibodies on Cecal Colonization and Organ Invasion of Salmonella Enteritidis in Broilers

Abstract

Salmonella Enteritidis colonizes the intestinal tract of poultry and causes foodborne illness in humans. Reduction of Salmonella Enteritidis colonization in the intestinal tract of poultry reduces potential carcass contamination during slaughter. The purpose of this study was to determine the effect of an avian-specific probiotic combined with Salmonella Enteritidis-, Salmonella Typhimurium-, and Salmonella Heidelberg-specific antibodies on the cecal colonization and organ invasion of Salmonella Enteritidis in broiler as well as on body weights. The treatment group was defined as chicks spray-vaccinated with Avian Pac Plus at the hatchery and given Avian Pac Plus for the first 3 days after placement. An intermediate treatment was given at 10 and 14 days, 2 days prior to vaccination and 2 days postvaccination. All birds were vaccinated with Newcastle disease vaccine, La Sota virus (one drop/eye) at 12 days of age. A final treatment was given 3 days preslaughter. The control group was defined as chicks not given Avian Pac Plus at any time. Six hours after oral administration of the probiotic suspension (treatment group) or water (control group) at placement, the chicks were challenged with Salmonella Enteritidis. All chickens were orally inoculated with 0.25 ml of Salmonella Enteritidis that contained 4 x 10(7) CFU/1.0 ml. Cecal colonization and organ invasion were evaluated for Salmonella Enteritidis on days 0, 1, 3, 7, 10, 17, 24, 31, 38, and 41. The probiotic-treated group had a significantly lower concentration of Salmonella Enteritidis cecal colonization at days 3, 7, 10, 17, 24, 31, 38, and 41 when compared to the nontreated, control group (P < 0.05). Similarly, there was a significant difference (P < 0.05) in the isolation of Salmonella Enteritidis from the internal organs (liver and spleen) when probiotic-treated and nonprobiotic-treated groups were compared. There was no significant difference (P > 0.05) in the mean body weight between the two experimental groups at each collection period. These results indicated that a combination of Lactobacillus acidophilus, Streptococcus faecium, and Salmonella Enteritidis, Salmonella Typhimurium, and Salmonella Heidelberg-Specific antibodies have a beneficial effect in reducing the colonization of Salmonella Enteritidis in market-aged broilers.

A Trial to Prevent Salmonella Enteritidis Infection in Broiler Chickens Using Autogenous Bacterin Compared with Probiotic Preparation

“…Concerning the reducing effect of the probiotics on S. Enteritidis shedding rate, the results of Tellez et al, (2001) and Rahimi et al, (2007) in broilers and Fulton et al, (2002) in duckling are parallel with our results.…”

“…Probiotics consist of live or dead organisms and spores (Patterson and Burkholder 2003), non-traditional chemicals (Moore et al, 2006), bacteriophages (Higgins et al, 2005), organic acids (Jarquin et al, 2007;Wolfenden et al, 2007) and others have emerged in the last decades as some of the tools that could be potentially useful in the near future for pathogen control and poultry performance. Probiotics are used now safely to prevent enteric infections especially with S. Enteritidis in newly hatched chicks with successful results (Soerjadi et al, 1981;Fuller 1997;Tellez et al, 2001;Ayed et al, 2004;Wafaa et al, 2006;Higgins et al, 2007a, b;Revolledo et al, 2009). …”

The effects of Yeasture® at different rearing stages on intestinal microflora and blood biochemical parameters in male and female broiler chickens

“…In similar case, Murry et al (2006) found lower Coliforms bacteria count in birds by feeding probiotics. Therefore, the beneficial microorganisms establish in the digestive tract and prevent the pathogens (Tellez et al, 2001;La Ragione et al, 2004). Also, in our experiment, Bifidobacterium count was higher at whole period (1 to 42 days).…”

Investigação de Salmonella spp., Listeria spp., Campylobacter spp. e Pseudomonas spp. no trato intestinal de avestruzes (Struthio camelus) criados no estado do Rio de Janeiro, Brasil

“…Estes resultados coincidem com os de outros autores que também não isolaram estes agentes a partir de avestruzes (Ley et al, 2001;Melville et al, 2004). Nos animais jovens investigados nesse estudo, o não isolamento destes micro-organismos pode ser devido à utilização de probióticos no primeiro dia de vida, o que evita a colonização de patógenos, dificultando a sua detecção por métodos tradicionais (Andreatti Filho et al, 2000;Tellez et al, 2001). Redução significativa na excreção de Salmonella Typhimurium foi obtida por Lopes et al, (2007), nos casos de enterites associadas à mortalidade em avestruzes, após administração de bactérias da microbiota entérica de avestruzes para aves recém-nascidas.…”