Background:The major part of demand for blood in India is met through voluntary blood donations. Students consists a large and healthy group who are able to provide a large number of blood donation. However, there is a paucity of studies on knowledge and attitude among undergraduate students from medical and paramedical branches. Objectives: A present study was conducted to assess the knowledge and attitude about blood donation among undergraduate medical science university students. Materials and Methods: A cross-sectional study was conducted among 400 final year undergraduate students from medical, dental, nursing, and physiotherapy disciplines in a Pravara institute of Medical Sciences University campus of central India during the period of May-August 2011. Data was analyzed in the form of percentage and proportions and Chi-square test. Results: The overall knowledge on blood donation was good; however, majority (52.5%) of students never donated blood. Knowledge level was found highest among medical students (53.1%) and lowest among physiotherapy students (20.7%). Non-consideration, forgetfulness, and lack of time were the major reasons for not donating blood. A significant association was observed between different streams of students and levels of knowledge and attitude about blood donation. Conclusion: This study elicits the importance of adopting effective measures in our campus to motivate about voluntary blood donation among students.